Zinc attenuates forskolin-stimulated electrolyte secretion without involvement of the enteric nervous system in small intestinal epithelium from weaned piglets

Serosal Zn2+ inhibits 8-Br-cAMP stimulated chloride secretion in piglet small intestinal epithelium in vitro

“…The performance and zinc status data agree with our previous findings in pigs fed diets containing 100 and 2500 ppm of zinc from ZnO (Carlson et al, 2008;Feng et al, 2006). Consequently, the data do not indicate a lower zinc status of the piglets fed a diet with no zinc supplement (ZnO 0 ) in this study compared with pigs fed a diet with 100 ppm of zinc in our previous studies.…”

“…The exact mechanism behind the effect is still not completely understood. In vitro studies have shown that Zn 2+ present at the basolateral membrane reduces the effects of some secretagogues (5-HT and FSK) in small intestinal epithelium (Carlson et al, 2008;Feng et al, 2006), and apparently the enteric nerve system is not involved in this attenuating effect of Zn 2+ (Feng et al, 2006). Hoque et al (2005) found that Zn 2+ inhibits cAMP stimulated Cl − secretion in rat ileal epithelium and suggested that the mechanism was a blockade of basolateral K + channels.…”

Zinc is involved in regulation of secretion from intestinal epithelium in weaned piglets

“…In the present study plasma Zn increased with dietary Zn, whereas ADG was not significantly affected (Feng et al, 2006). However, in our previous study there was a significant and more pronounced effect of dietary Zn on ADG and plasma Zn concentrations.…”

“…Least square means for ADG, plasma Zn, mucosa Zn and liver Zn concentrations in exp 2 are presented elsewhere (Feng et al, 2006).…”

Mechanisms of action of zinc on rat intestinal epithelial electrogenic ion secretion: insights into its antidiarrhoeal actions

“…Zinc also blocked the inhibition of short circuit current (I sc ) stimulated by 5‐hydroxytryptamine (5‐HT), vasoactive intestinal peptide (VIP) and carbachol in the isolated small intestine of piglets, an effect requiring high basolateral concentrations [8,9] . Anti‐secretory effects of zinc on forskolin‐induced I sc in pig intestine were directly at the epithelium and did not involve neurotransmitter release from the enteric nervous system [10] . Importantly, zinc blocked cAMP‐stimulated 86 Rb efflux from rat isolated ileal crypt cells, suggesting involvement of K channels.…”