Zinc availability regulates exit from meiosis in maturing mammalian oocytes

Kim, Alison M.; Vogt, Stefan; O’Halloran, Thomas V.; Woodruff, Teresa K.

doi:10.1038/nchembio.419

Cited by 227 publications

(270 citation statements)

References 43 publications

Supporting

Mentioning

249

Contrasting

Order By: Relevance

“…This finding is in agreement with that of de los Santos, where ZNF33B was up-regulated in MNIVF cycles. Zinc finger protein has been shown to be involved in the regulation of meiosis in mammalian oocytes [53]. These findings warrant further studies in order to determine the role of zinc finger protein family in human ovarian folliculogenesis.…”

Section: Discussionmentioning

confidence: 86%

Differences in cumulus cells gene expression between modified natural and stimulated in vitro fertilization cycles

Papler

Bokal

Tacer

et al. 2013

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The aim of our study was to determine whether there are any differences in the cumulus cell gene expression profile of mature oocytes derived from modified natural IVF and controlled ovarian hyperstimulation cycles and if these changes could help us understand why modified natural IVF has lower success rates. Methods Cumulus cells surrounding mature oocytes that developed to morulae or blastocysts on day 5 after oocyte retrieval were submitted to microarray analysis. The obtained data were then validated using quantitative real-time PCR. Results There were 66 differentially expressed genes between cumulus cells of modified natural IVF and controlled ovarian hyperstimulation cycles. Gene ontology analysis revealed the oxidation-reduction process, glutathione metabolic process, xenobiotic metabolic process and gene expression were significantly enriched biological processes in MNIVF cycles.Among differentially expressed genes we observed a large group of small nucleolar RNA's whose role in folliculogenesis has not yet been established. Conclusion The increased expression of genes involved in the oxidation-reduction process probably points to hypoxic conditions in modified natural IVF cycles. This finding opens up new perspectives for the establishment of the potential role that oxidation-reduction processes have in determining success rates of modified natural IVF.

show abstract

Section: Discussionmentioning

confidence: 86%

Differences in cumulus cells gene expression between modified natural and stimulated in vitro fertilization cycles

Papler

Bokal

Tacer

et al. 2013

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…The increase in oocyte zinc reported in the literature (Kim et al 2010 suggests that cumulus cells lose the ability to suppress free intracellular zinc in the oocyte or that maturing oocytes become insensitive to cumulus cells. To test these possibilities, denuded oocytes were cultured alone, with intact COC or with COC that had been pretreated with EGF (10 ng/ml) for 4 h to initiate COC maturation.…”

Section: Changes In Suppressive Properties Of Cumulus Cells Vs Changementioning

confidence: 89%

“…Cumulus cells regulate the timing of the increase in free intracellular zinc in the oocyte during maturation. This effect of cumulus cells is important because an increase in oocyte zinc is required for completion of meiosis (Kim et al 2010 and successful establishment of MII arrest (Suzuki et al 2010b. Thus, acute regulation of free intracellular zinc is a new process regulated by cumulus cell-oocyte interactions in the follicle.…”

Section: Discussionmentioning

confidence: 99%

“…Total zinc, including zinc bound to proteins, is very abundant in the oocyte before maturation (Kim et al 2010) and is involved in preventing premature germinal vesicle (GV) breakdown in GV-stage oocytes , Tian & Diaz 2012. Total zinc increases during maturation and is required for the completion of the first meiotic division (Kim et al 2010), although this is independent of the MOS-MAPK pathway . Zinc is also required for establishment of metaphase II (MII) arrest through the regulation of the zinc-binding protein early meiosis inhibitor 2 (EMI2) (Suzuki et al 2010b.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Oocyte–cumulus cell interactions regulate free intracellular zinc in mouse oocytes

Lisle¹,

Anthony²,

Randall³

et al. 2013

Reproduction

View full text Add to dashboard Cite

Zinc increases in the oocyte during maturation and is required for progression and completion of meiosis. The objective of this study was to determine whether cumulus cells regulate the levels of free intracellular zinc in the oocyte during maturation. In the cumulus-oocyte complex (COC) the relative level of free intracellular zinc was almost fourfold higher in cumulus cells compared with the resident germinal vesicle-stage oocyte. Removal of cumulus cells caused a fourfold increase in intracellular zinc in the oocyte by 1 h after cumulus cell removal, but subsequent coculture of denuded oocytes with COC decreased free intracellular zinc in the oocyte by 65%. Thus, cumulus cells suppress free intracellular zinc in the oocyte. The mRNA transcripts for the zinc transporter proteins Slc39a6, Slc39a8, Slc39a9, Slc39a10, Slc39a12, Slc30a2, Slc30a4, Slc30a5 and Slc30a8 mRNAs were higher in oocytes, while Slc39a1, Slc39a7, Slc39a13, Slc39a14, Slc30a6, Slc30a7 and Slc30a9 mRNAs were higher in cumulus cells. Thus a complex zinc transport network is present in the COC. Pretreatment with epidermal growth factor for 4 h abolished the ability of COCs to restrict free intracellular zinc in denuded oocytes. Coculture of denuded metaphase II oocytes with COC lowers free intracellular zinc in mature oocytes. Oocytes matured in vivo or oocytes from older mice had lower levels of free intracellular zinc than oocytes matured in vitro or from younger mice. Thus, a precise mechanism for regulating oocyte zinc homeostasis has been uncovered in the COC that is disrupted with increasing age or by removal of cumulus cells.

show abstract

“…Compared with "water window" soft X-ray tomography (30,58,59), tomography with multi-keV X-rays offers the capability for increased sample thickness, and increased depth of focus for a given spatial resolution, which allows this combined method to image larger cells and tissue sections in 3D. This method will aid the interpretation of studies of the localization of nanoparticles attached to therapeutic agents (1,60), or the role of metals in cell development (61), and in diseases where trace metal misregulation is implicated as a cause (62). One could of course carry out separate experiments where one first does a fluorescence scan with a finely focused beam, followed by a separate ptychographic imaging experiment with a larger beam spot and fewer illumination points; this would however involve a longer time for the experiment if done in one instrument, or risk of specimen frosting if one instead transferred the cryo sample to a separate instrument.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae

Deng

Vine

Chen

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

164

131

View full text Add to dashboard Cite

Trace metals play important roles in normal and in disease-causing biological functions. X-ray fluorescence microscopy reveals trace elements with no dependence on binding affinities (unlike with visible light fluorophores) and with improved sensitivity relative to electron probes. However, X-ray fluorescence is not very sensitive for showing the light elements that comprise the majority of cellular material. Here we show that X-ray ptychography can be combined with fluorescence to image both cellular structure and trace element distribution in frozen-hydrated cells at cryogenic temperatures, with high structural and chemical fidelity. Ptychographic reconstruction algorithms deliver phase and absorption contrast images at a resolution beyond that of the illuminating lens or beam size. Using 5.2-keV X-rays, we have obtained sub-30-nm resolution structural images and ∼90-nm-resolution fluorescence images of several elements in frozen-hydrated green algae. This combined approach offers a way to study the role of trace elements in their structural context. ptychography | X-ray fluorescence microscopy | cryogenic biological samples X -ray fluorescence microscopy (XFM) offers unparalleled sensitivity for quantitative mapping of elements, especially trace metals which play a critical role in many biological processes (1-3). It is complementary to light microscopy, which can study some elemental content in live cells (with superresolution techniques possible) but which is more difficult to quantitate because it depends on the binding affinities of fluorophores. However, XFM does not usually show much cellular ultrastructure, because the light elements (such as H, C, N, and O, which are the main constituents of biological materials) have low fluorescence yield (4). At the multi-keV X-ray energies needed to excite most X-ray fluorescence lines of interest, these light elements show little absorption contrast, but phase contrast can be used to image cellular structure (5, 6) and this can be combined with scanned-beam XFM (7-11).One can also acquire phase-contrast X-ray images with a resolution beyond X-ray lens limits by recording the diffraction pattern from a coherently illuminated, noncrystalline sample in an approach called coherent diffraction imaging (CDI) (12). This approach has been used to image isolated dried cells (13-15), and 3-nm resolution has been achieved when imaging silver nanocubes (16). The traditional CDI approach requires that samples meet a so-called "finite support" (17) requirement with no observable scattering outside of a defined region; although some limited success has been obtained (18,19), this finite support condition has proven difficult to achieve with single cells surrounded by ice layers. Ptychography (20-22) is a recently realized CDI method [with an older history (23)] that circumvents this isolated cell requirement by instead scanning a limitedsize coherent illumination spot across the sample. Ptychography has been used to image freeze-dried diatoms at 30-nm resolution (24) and bacter...

show abstract

Zinc availability regulates exit from meiosis in maturing mammalian oocytes

Cited by 227 publications

References 43 publications

Differences in cumulus cells gene expression between modified natural and stimulated in vitro fertilization cycles

Differences in cumulus cells gene expression between modified natural and stimulated in vitro fertilization cycles

Oocyte–cumulus cell interactions regulate free intracellular zinc in mouse oocytes

Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae

Contact Info

Product

Resources

About