Zinc-Coated Carbonate Apatite Derived From Avian Eggshell for Potential Use as Bone Substitute. Part I

Cai, Yi-Da; Wang, Shumin; Chou, Alex Hung Kuo; Yu, Liangli; Sun, Jiao

doi:10.1097/id.0b013e3182563ce5

Cited by 8 publications

(3 citation statements)

References 26 publications

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“…6,15,16 Nano-hydroxyapatite synthesized from eggshell biowaste showed promising results in vitro. 6,15–17 Clinical studies have used nano-HA derived from eggshell waste as a graft substitute in apicectomy and extraction defects successfully. 18–21…”

Section: Introductionmentioning

confidence: 99%

Use of eggshell-derived nano-hydroxyapatite as novel bone graft substitute–A randomized controlled clinical study

et al. 2019

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Background Use of various bone graft substitutes are advised to prevent bone loss, periodontal problems, and enhance bone formation after surgical removal of the mandibular third molar. Choice of graft substitute depends on biological properties and the cost of the material. So, a preliminary study was planned to evaluate and compare the effectiveness of eggshell-derived nano-hydroxyapatite as an emerging graft substitute for the enhancement of bone regeneration. Materials and methods Twelve patients (out of 20 enrolled) were finally assessed for bone healing after surgical removal of bilateral mandibular third molars in a split-mouth randomized controlled clinical study. All of them were assessed and compared at baseline, first, third, and sixth month. Clinically wound healing and probing depth were assessed. Radiographically the changes in density, bone formation characteristics, and bone level were evaluated. The observers were blinded for the surgical procedure and follow-up duration. The study protocol was approved by Institutional Ethics Committee and registered with National clinical trial registry. Results Wound healing was uneventful in all 24 extraction sites of 12 patients. Trabecular bone pattern with increased bone density was observed indicating complete bone healing in the graft group at third month. Probing depth was gradually decreased over a follow-up period and remained within normal limits during the third-month follow-up. These changes in the graft group were significant compared to the control group. The grafting with eggshell-derived nano-hydroxyapatite showed better bone regeneration properties without any infection or undue reactions. Conclusion Eggshell-derived nano-hydroxyapatite showed enhancement of bone regeneration compared to the control group. Grafting maintained bone height and prevented periodontal problem emergence. Eggshell-derived nano-hydroxyapatite is a safe synthetic graft substitute because it is derived from eggshell without any diseases transfer risks, unlike allografts. The process of preparation is environment-friendly. The beneficial effects of eggshell-derived nano-hydroxyapatite may be extrapolated to develop an ideal economic graft substitute.

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Section: Introductionmentioning

confidence: 99%

Use of eggshell-derived nano-hydroxyapatite as novel bone graft substitute–A randomized controlled clinical study

et al. 2019

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“…Within the context of mineralizing peri-implant tissue, it is proposed that, presence of Zn in association with P could be linked to three parallel or serially occurring scenarios: 1) biomineralization through pyrophosphate (PPi) and phosphate (Pi) (PPi-Pi) axis [36] that is facilitated by tissue nonspecific alkaline phosphatase whose function is permitted by Zn; 2) inflammation-mediated wound healing pathway through matrix metalloproteinases whose function also is mediated by Zn [37]; 3) presence of osteogenic cells with expression of zinc-finger proteins such as osterix [38]. Of the three elements localized in this study, Zn in particular, has been demonstrated to be an osteoconductive element [39][40][41][42]. Its presence is likely under osteoinductive conditions, mechanical loads included.…”

Section: Discussionmentioning

confidence: 99%

Mechanoadaptive strain and functional osseointegration of dental implants in rats

Wang¹,

Kim²,

Srirangapatanam

et al. 2020

Bone

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Spatiotemporal implant-bone biomechanics and mechanoadaptive strains in peri-implant tissue are poorly understood. Physical and chemical characteristics of an implant-bone complex (IBC) were correlated in three-dimensional space (along the length and around a dental implant) to gather insights into time related integration of the implant with the cortical portion of jaw bone in a rat. Rats (N = 9) were divided into three experimental groups with three rats per time point 3-, 11-, and 24-day.All rats were fed crumbled hard pellets mixed with water (soft-food diet) for the first 3 days followed by a hard-food diet with intact hard-food pellets (groups of 11-and 24-day only).Biomechanics of the IBCs harvested from rats at each time point was evaluated by performing mechanical testing in situ in tandem with X-ray imaging. The effect of physical association (contact area) of a loaded implant with adapting peri-implant tissue, and resulting strain within was mapped by using digital volume correlation (DVC) technique. The stiffness of the IBC at respective time points was correlated with mechanical strain in bone. Results illustrated that the stiffness of the IBC at 11-day was lower than that observed at 3-day. However, at 24-day, IBC stiffness recovered to that which was observed at 3-day. Correlative microscopy and spectroscopy illustrated that the lower IBC stiffness was constituted by softer and less mineralized peri-implant tissue that contained varying expressions of osteoconductive elements. Lower stiffness observed at 11-day was constituted by less mineralized peri-implant tissue with osteoconductive elements that included phosphorus (P) which was co-localized with higher expression of zinc (Zn), and lower expression of calcium (Ca). Higher stiffness at 24-day was constituted by mineralized peri-implant tissue with higher expressions of osteoconductive elements including Ca and P, and lower expressions of Zn.These spatiotemporal correlative maps of peri-implant tissue architecture, heterogeneous distribution of mineral density, and elemental colocalization underscore mechanoadaptive physicochemical properties of peri-implant tissue that facilitate functional osseointegration of an implant. These results provided insights into 1) plausible "prescription" of mechanical loads as an osteoinductive "therapeutic dose" to encourage osteoconductive elements in the IBC that would facilitate functional osseointegration of the implant; 2) a "critical temporal window" between 3 to 11 days, and perhaps it is this acute phase during which key candidate regenerative molecules can be harnessed to accelerate osseointegration of an implant under load.

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“…Upcoming studies may use other processing methods of the eggshell, like surface-modified carbonated apatite [36,37], eggshell microparticle (ESP) reinforced gelatin-based hydrogels [38], or eggshells from other sources like ostriches [39]. Another possibility would be to combine eggshells and bioactive materials such as recombinant human bone morphogenetic protein-2 (rhBMP-2), as it was done in other recent studies [40,41].…”

Section: Wwwbjbmsorgmentioning

confidence: 99%

Clinical applications of avian eggshell-derived hydroxyapatite

Opriș

Bran

Dinu

et al. 2020

Bosn J of Basic Med Sci

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The search for bone reconstruction materials and methods is an ongoing challenge. The aim of this review is to systemically search the available literature concerning the clinical performance of eggshell as a substitute material in guided bone regeneration in oral surgery. Five databases (PubMed, Cochrane, Web of Science, Scopus, and Embase) were searched up to February 2020. Clinical trials that used eggshell as a bone substitute material were included. Animal and in vivo studies were excluded. ROBINS-I was used to evaluate the risk of bias. A total of 840 studies were retrieved, out of which 55 full-text articles were screened. Five studies were finally included: one study showed critical and four serious risk of bias. A total of 74 patients and 88 intervention sites were included in the five studies. Clinical and radiological evaluation showed complete healing during the follow-ups. Statistically significant radiological and clinical evidence of new bone formation was achieved for socket preservation, grafting after third molar extraction, and cystic/apicectomy grafting. One patient with complications was reported. Histological analysis and micro computed tomography confirmed that it promotes bone regeneration. A comparison with synthetic hydroxyapatite showed similar healing characteristics. Within the limitations of the included studies, eggshell can be safely and efficiently used in guided bone regeneration procedures, but more research is needed to completely evaluate the full potential of this material.

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Zinc-Coated Carbonate Apatite Derived From Avian Eggshell for Potential Use as Bone Substitute. Part I

Cited by 8 publications

References 26 publications

Use of eggshell-derived nano-hydroxyapatite as novel bone graft substitute–A randomized controlled clinical study

Use of eggshell-derived nano-hydroxyapatite as novel bone graft substitute–A randomized controlled clinical study

Mechanoadaptive strain and functional osseointegration of dental implants in rats

Clinical applications of avian eggshell-derived hydroxyapatite

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