Zinc-Deficient Diet Causes Imbalance in Zinc Homeostasis and Impaired Autophagy and Impairs Semen Quality in Mice

Sun, Bo; Ma, Jing; Te, Liger; Zuo, Xin; Liu, Junsheng; Li, Yuejia; Bi, Jiajie; Wang, Shusong

doi:10.1007/s12011-022-03324-1

Cited by 15 publications

(2 citation statements)

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“…Zn ions can interact with spermatozoa, some functional groups of the basic components of the sperm membrane, and protect the lipid bilayer from the peroxidation process during and after cryopreservation, thus improving the plasma membrane integrity of spermatozoa after cryo-resuscitation [ 18 ]. This is consistent with previous research that showed mitochondria are one of the most important organelles and could be damaged by the freezing-thawing process, which results in higher ROS production [ 9 , 10 ].…”

Section: Discussionsupporting

confidence: 93%

“…With its moderate reactivity and reducing characteristics, Zn is a required cofactor for several metalloenzymes and acts as a catalyst for many enzymes [ 8 ] . Our team has been working on research related to Zn and male reproduction and has reported that Zn is involved in testicular growth, sperm quality and functional parameters, and male fertility [ 9 , 10 ]. In some animal studies, Zn can act as an antioxidant and improve sperm quality after cryopreservation [ 3 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of Frozen-Thawed Human Sperm Quality with Zinc as a Cryoprotective Additive

Fu,

Ma,

Chen

et al. 2024

Med Sci Monit

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Background Cryopreservation preserves male fertility, crucial in oncology, advanced age, and infertility. However, it damages sperm motility, membrane, and DNA. Zinc (Zn), an antioxidant, shows promise in improving sperm quality after thawing, highlighting its potential as a cryoprotectant in reproductive medicine. Material/Methods Gradient concentration of ZnSO 4 (0, 12.5, 25, 50, and 100 μM) was added in the Glycerol-egg yolk-citrate (GEYC) cryopreservative medium as an extender. Alterations in sperm viability and motility parameters after cryopreservation were detected in each group. Sperm plasma membrane integrity (PMI), acrosome integrity (ACR), DNA fragment index (DFI), and changes in sperm mitochondrial function were examined, including: mitochondrial potential (MMP), sperm reactive oxygen species (ROS), and sperm ATP. Results We found that 50 μM ZnSO 4 was the most effective for the curvilinear velocity (VCL) and the average path velocity (VAP) of sperm after cryo-resuscitation. Compared to the Zn-free group, sperm plasma membrane integrity (PMI) was increased, DNA fragmentation index (DFI) was decreased, reactive oxygen species (ROS) was reduced, and mitochondrial membrane potential (MMP) was increased after cryorevival in the presence of 50 μM ZnSO 4 . Conclusions Zn ion is one of the antioxidants in the cell. The results of our current clinical study are sufficient to demonstrate that Zn can improve preserves sperm quality during cryopreservation when added to GEYC. The addition of 50 μM ZnSO 4 increased curve velocity, mean path velocity, sperm survival (or plasma membrane integrity), and mitochondrial membrane potential while reducing ROS production and DNA breaks compared to GEYC thawed without ZnSO 4 .

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Section: Discussionsupporting

confidence: 93%