Zinc deficient diet exacerbates the testicular and epididymal damage in type 2 diabetic rat: Studies on oxidative stress-related mechanisms

Maremanda, Krishna Prahlad; Srivalliputturu, Sarath Babu; Jena, Gopabandhu

doi:10.1016/j.repbio.2020.03.002

Cited by 11 publications

(6 citation statements)

References 38 publications

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“…This study has demonstrated the hypoglycaemic effect of D. serrulata flower (DSF) on male reproductive damages in T2DM rats induced with high-fat diet and streptozotocin for the first time. For animal model of T2DM, our results were consistent with previous reports showing significant increase of BGL in T2DM rats (Choubey et al, 2020;Magalhães et al, 2019;Maremanda et al, 2020;Srinivasan et al, 2005;Taylor, 2012). It has demonstrated that protocatechuic acid (PCA) could reduce the BGL in DM animal models (Harini & Pugalendi, 2010;Ma et al, 2018).…”

Section: Discussionsupporting

confidence: 92%

“…Indeed, several investigations have also demonstrated the significant decrease of male reproductive organ weights in T2DM rats (Sampannang et al, 2019(Sampannang et al, , 2020Yannasithinon & Iamsaard, 2019). Similar to many studies, we also demonstrated detrimental effect of DM on sperm parameters (La Vignera et al, 2012;Liu et al, 2013;Maremanda et al, 2020;Sampannang et al, 2020). It was assumed that antioxidants in DFS could protect and recover sperm qualities including sperm acrosome reaction, abnormal morphology, incomplete DNA structure and packaging and chromatin de-condensation in T2DM animals.…”

Section: Discussionsupporting

confidence: 88%

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Dolichandrone serrulata flower extract ameliorates male reproductive damages in type 2 diabetic rats

et al. 2020

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Dolichandrone serrulata flower (DSF) has been believed to reduce blood glucose in hyperglycaemic persons with sub-fertility but its effect on improvement of male reproductive impairment has never been elucidated scientifically. This study attempted to investigate the hypoglycaemic effects of DSF on male reproductive damages in type 2 diabetes mellitus (T2DM) rats. Adult Sprague Dawley rats were divided into four groups (control, T2DM, DSF200 + T2DM and DSF600 + T2DM; n = 10/each). Control rats received low-fat diet for 14 days before saline injection while streptozocin (50 mg/kg BW) induced T2DM groups received high-fat diet and were orally administered with DSF (200 and 600 mg/kg BW) for 28 days. At the end, fasted blood glucose (FBG), malondialdehyde (MDA), testosterone, sperm quality, histology and protein expressions were examined. The result showed that DSF decreased high FBG and testicular MDA and increased testosterone levels of T2DM-treated rats. Lowsperm quality and histological malfunction were ameliorated in DSF-treated group. There was significant decrease in the expression of androgen receptor, heat-shock 70 and steroidogenic acute regulatory proteins of T2DM-treated rats. Our study demonstrated changes of six bands (116, 51, 45, 39, 35 and 29 kDas) of tyrosinephosphorylated proteins. In conclusion, DSF could reduce the FBGand ameliorate the reproductive damages in male T2DM rats.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 88%

Dolichandrone serrulata flower extract ameliorates male reproductive damages in type 2 diabetic rats

et al. 2020

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“…A mouse model of type 2 diabetes can be induced by a HFD and a low dose of STZ (Liu et al, 2019;Maremanda et al, 2020). In the present study, mice were fed with a HFD for 12 weeks prior to the injection of STZ.…”

Section: The Combination Of Stz and A Hfd Successfully Induced A Model Of Type 2 Diabetesmentioning

confidence: 98%

NmFGF1-Regulated Glucolipid Metabolism and Angiogenesis Improves Functional Recovery in a Mouse Model of Diabetic Stroke and Acts via the AMPK Signaling Pathway

Zhao

Lin

et al. 2021

Front. Pharmacol.

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Diabetes increases the risk of stroke, exacerbates neurological deficits, and increases mortality. Non-mitogenic fibroblast growth factor 1 (nmFGF1) is a powerful neuroprotective factor that is also regarded as a metabolic regulator. The present study aimed to investigate the effect of nmFGF1 on the improvement of functional recovery in a mouse model of type 2 diabetic (T2D) stroke. We established a mouse model of T2D stroke by photothrombosis in mice that were fed a high-fat diet and injected with streptozotocin (STZ). We found that nmFGF1 reduced the size of the infarct and attenuated neurobehavioral deficits in our mouse model of T2D stroke. Angiogenesis plays an important role in neuronal survival and functional recovery post-stroke. NmFGF1 promoted angiogenesis in the mouse model of T2D stroke. Furthermore, nmFGF1 reversed the reduction of tube formation and migration in human brain microvascular endothelial cells (HBMECs) cultured in high glucose conditions and treated with oxygen glucose deprivation/re-oxygenation (OGD). Amp-activated protein kinase (AMPK) plays a critical role in the regulation of angiogenesis. Interestingly, we found that nmFGF1 increased the protein expression of phosphorylated AMPK (p-AMPK) both in vivo and in vitro. We found that nmFGF1 promoted tube formation and migration and that this effect was further enhanced by an AMPK agonist (A-769662). In contrast, these processes were inhibited by the application of an AMPK inhibitor (compound C) or siRNA targeting AMPK. Furthermore, nmFGF1 ameliorated neuronal loss in diabetic stroke mice via AMPK-mediated angiogenesis. In addition, nmFGF1 ameliorated glucose and lipid metabolic disorders in our mouse model of T2D stroke without causing significant changes in body weight. These results revealed that nmFGF1-regulated glucolipid metabolism and angiogenesis play a key role in the improvement of functional recovery in a mouse model of T2D stroke and that these effects are mediated by the AMPK signaling pathway.

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“…A similar modelling method was used by Maremanda et al to show that a zinc-deficient diet exacerbates testicular and epididymal damage in type 2 diabetic rats. (Maremanda et al, 2020). Recent studies have assessed the impact of et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Testicular damage caused by diabetes is one of the leading causes of male infertility (Abdel‐Aziz et al, 2020). Male infertility includes erectile dysfunction, ejaculation disorders, azoospermia, testicular damage and other intricate causes, including physiological and psychological aspects (Choubey et al, 2020; Lewis et al, 2010; Maremanda et al, 2020). Endocrine disorders, varicoceles and sexual problems such as erectile dysfunction and ejaculation disorders, can be corrected with medication and psychological intervention (Lewis et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Cordycepin, a major bioactive component ofCordyceps militaris, ameliorates diabetes‐induced testicular damage through the Sirt1/Foxo3a pathway

Huang

Zhou

et al. 2021

Andrologia

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Diabetes‐induced male dysfunction is considered as a worldwide challenge, and testicular damage mainly caused by oxidative stress is its most common manifestation. Cordycepin, a natural antioxidant, has been used in the treatment of diabetic complications. However, the protective action and underlying mechanism of cordycepin on hyperglycaemia‐induced testicular damage are unclear. This study aimed to investigate the protective effects and molecular mechanisms of cordycepin against diabetes‐induced testicular damage. The type 2 diabetes model was established in C57BL/6 male mice via high‐fat diet for 4 weeks and injected intraperitoneally with 50 mg/kg/day streptozotocin for five consecutive days. Then mice were treated with cordycepin (10 and 20 mg/kg, respectively) for 8 weeks. At the end of experiment, biochemical indicators, microstructure of testicular tissue, sperm morphology, TUNEL staining and protein expressions were evaluated. In the present study, cordycepin alleviated the testicular damage, restored disruption of the blood‐testis barrier, and improved spermatogenic function via the antiapoptotic and antioxidant capacity. Mechanistically, cordycepin significantly enhanced SIRT1 expression and triggered the activity of Foxo3a, further to induce the expression of its downstream antioxidant enzymes, including Mn‐SOD and CAT. These findings indicated that cordycepin could improve hyperglycaemia‐induced testicular damage by regulating downstream antioxidant enzymes activity through the SIRT1/Foxo3a signalling pathway.

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Zinc deficient diet exacerbates the testicular and epididymal damage in type 2 diabetic rat: Studies on oxidative stress-related mechanisms

Cited by 11 publications

References 38 publications

Dolichandrone serrulata flower extract ameliorates male reproductive damages in type 2 diabetic rats

Dolichandrone serrulata flower extract ameliorates male reproductive damages in type 2 diabetic rats

NmFGF1-Regulated Glucolipid Metabolism and Angiogenesis Improves Functional Recovery in a Mouse Model of Diabetic Stroke and Acts via the AMPK Signaling Pathway

Cordycepin, a major bioactive component ofCordyceps militaris, ameliorates diabetes‐induced testicular damage through the Sirt1/Foxo3a pathway

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