ZINC-FINGER interactions mediate transcriptional regulation of hypocotyl growth in
            <i>Arabidopsis</i>

Perrella, Giorgio; Davidson, Mhairi L. H.; O’Donnell, Liz; Nastase, Ana-Marie; Herzyk, Paweł; Breton, Ghislaı̀n; Pruneda‐Paz, Jose L.; Kay, Steve A.; Chory, Joanne; Kaiserli, Eirini

doi:10.1073/pnas.1718099115

Cited by 36 publications

(36 citation statements)

References 73 publications

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“…The zinc-finger family proteins have been implicated in transcriptional regulation [28, 29]. Thus, we fused the full length ZMYM1 to the DNA-binding domain of Gal4 and tested its transcriptional activity in GC cells.…”

Section: Resultsmentioning

confidence: 99%

METTL3-mediated N6-methyladenosine modification is critical for epithelial-mesenchymal transition and metastasis of gastric cancer

et al. 2019

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Background As one of the most frequent chemical modifications in eukaryotic mRNAs, N6-methyladenosine (m6A) modification exerts important effects on mRNA stability, splicing, and translation. Recently, the regulatory role of m6A in tumorigenesis has been increasingly recognized. However, dysregulation of m6A and its functions in tumor epithelial-mesenchymal transition (EMT) and metastasis remain obscure. Methods qRT-PCR and immunohistochemistry were used to evaluate the expression of methyltransferase-like 3 (METTL3) in gastric cancer (GC). The effects of METTL3 on GC metastasis were investigated through in vitro and in vivo assays. The mechanism of METTL3 action was explored through transcriptome-sequencing, m6A-sequencing, m6A methylated RNA immunoprecipitation quantitative reverse transcription polymerase chain reaction (MeRIP qRT-PCR), confocal immunofluorescent assay, luciferase reporter assay, co-immunoprecipitation, RNA immunoprecipitation and chromatin immunoprecipitation assay. Results Here, we show that METTL3, a major RNA N6-adenosine methyltransferase, was upregulated in GC. Clinically, elevated METTL3 level was predictive of poor prognosis. Functionally, we found that METTL3 was required for the EMT process in vitro and for metastasis in vivo. Mechanistically, we unveiled the METTL3-mediated m6A modification profile in GC cells for the first time and identified zinc finger MYM-type containing 1 (ZMYM1) as a bona fide m6A target of METTL3. The m6A modification of ZMYM1 mRNA by METTL3 enhanced its stability relying on the “reader” protein HuR (also known as ELAVL1) dependent pathway. In addition, ZMYM1 bound to and mediated the repression of E-cadherin promoter by recruiting the CtBP/LSD1/CoREST complex, thus facilitating the EMT program and metastasis. Conclusions Collectively, our findings indicate the critical role of m6A modification in GC and uncover METTL3/ZMYM1/E-cadherin signaling as a potential therapeutic target in anti-metastatic strategy against GC.

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Section: Resultsmentioning

confidence: 99%

METTL3-mediated N6-methyladenosine modification is critical for epithelial-mesenchymal transition and metastasis of gastric cancer

et al. 2019

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“…TANDEM ZINC-FINGER PLUS3 (TZP) was active in promoting hypocotyl elongation at the transcriptional level. At the same time, it showed the mechanism of the ZF-HD TFs family in promoting the growth of Arabidopsis [23]. The expression of TaZFHD1 is upregulated by different hormones during spike development [24].…”

Section: Introductionmentioning

confidence: 84%

Genome-wide analysis and expression characterization of zinc finger homeodomain (ZHD) family genes responsed to different abiotic stresses and hormonal treatments in grape (Vitis vinifera L.)

Nai

Mao

et al. 2020

Preprint

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Background Zinc finger homeodomain (ZHD) proteins transcription factors (TFs) family occupy a crucial role in regulating plant growth and development. The ZHD TFs family had been studied in diverse organisms such as humans, Drosophila, nematode worms and plants. Especially in plants there is an extensive exploration involved in photosynthesis, defense mechanisms and responses to stress. In this further research, we will provide a better understanding of the ZHD gene family in grape. Results In this study, a total of 12 VvZHD genes were identified from the Grape Genome. The exploration of detailed bioinformatics found that the full length of VvZHD genes ranges from 150 bp (base pair) to 26706 bp, isoelectric points (pIs) were 6.25–9.68, molecular weights (MWs) ranged from 10.28 kilodaltons (KDa) to 97.93 kDa. Also, analysis of phylogenetic evolution showed there existed a common ancestor between Arabidopsis and grape. Ka (Nonsynonymous) / Ks (Synonymous) was lower than 1 and strong collinearity showed there were tandem duplications of genes between Arabidopsis and grape. Codon bias levels of ZHD gene family were relatively higher overall. Gene chip data analysis of cabernet sauvignon seedlings showed VvZHD genes had different expression under different time of different treatments, and with the prolongation of treatment time, most of VvZHD genes were slightly downregulated. The qRT-PCR was carried out under different hormones and stresses to verify expression profiles in grape roots and leaves. Results revealed that most VvZHD genes had higher expressions in roots but lower expressions in leaves. Also, most of the VvZHD gene members were responsed with high expressions after the treatment of ABA, IAA and MeJA in roots. However, VvZHD9, VvZHD11 and VvZHD12 were highly expressed by inducing of PEG in leaves. Conclusions Through genome-wide analysis and expression characterization of ZHD family genes, results showed 12 members had the diversity of function, structure and unique expression characterization under various abiotic stress and hormone treatments. This study will provide us with a newer insight on ZHD gene family of grape and promote to explore the biological functions of ZHD genes in other plants.

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“…BiFC. BiFC experiments on transiently transformed Nicotiana benthamiana epidermal cells and protein extraction was performed as previously described (37). For protein detection, ∼100 mg of tissue was homogenized in 4× Laemmli protein sample buffer, boiled at 100°C, and 25 μL of the protein extract was separated on a 4 to 12% Bolt SDS/PAGE (ThermoFisher).…”

Section: Methodsmentioning

confidence: 99%

“…Yeast 2-Hybrid. Assays were performed as previously described (37). The yeast strain MaV203 was used for the auxotrophy and GUS assays according to the manufacturer's instructions (Invitrogen).…”

Section: Methodsmentioning

confidence: 99%

HISTONE DEACETYLASE 9 stimulates auxin-dependent thermomorphogenesis in Arabidopsis thaliana by mediating H2A.Z depletion

Woude

Perrella

Snoek

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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116

145

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Many plant species respond to unfavorable high ambient temperatures by adjusting their vegetative body plan to facilitate cooling. This process is known as thermomorphogenesis and is induced by the phytohormone auxin. Here, we demonstrate that the chromatin-modifying enzyme HISTONE DEACETYLASE 9 (HDA9) mediates thermomorphogenesis but does not interfere with hypocotyl elongation during shade avoidance. HDA9 is stabilized in response to high temperature and mediates histone deacetylation at the YUCCA8 locus, a rate-limiting enzyme in auxin biosynthesis, at warm temperatures. We show that HDA9 permits net eviction of the H2A.Z histone variant from nucleosomes associated with YUCCA8, allowing binding and transcriptional activation by PHYTOCHROME INTERACTING FACTOR 4, followed by auxin accumulation and thermomorphogenesis.

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ZINC-FINGER interactions mediate transcriptional regulation of hypocotyl growth in Arabidopsis

Cited by 36 publications

References 73 publications

METTL3-mediated N6-methyladenosine modification is critical for epithelial-mesenchymal transition and metastasis of gastric cancer

METTL3-mediated N6-methyladenosine modification is critical for epithelial-mesenchymal transition and metastasis of gastric cancer

Genome-wide analysis and expression characterization of zinc finger homeodomain (ZHD) family genes responsed to different abiotic stresses and hormonal treatments in grape (Vitis vinifera L.)

HISTONE DEACETYLASE 9 stimulates auxin-dependent thermomorphogenesis in Arabidopsis thaliana by mediating H2A.Z depletion

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