Zinc‐finger paralogues tsh and tio are functionally equivalent during imaginal development in Drosophila and maintain their expression levels through auto‐ and cross‐negative feedback loops

Bessa, José; Carmona, Lourdes; Casares, Fernando

doi:10.1002/dvdy.21808

Cited by 36 publications

(62 citation statements)

References 38 publications

(50 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…D″, arrow). We acknowledge that it is possible that the lack of repression of 10xSTAT-GFP in tshi clones could be explained by a redundant repression by tip-top, a Tsh paralogue that is expressed in an overlapping pattern to that of tsh (Bessa et al, 2009). We also monitored the expression of Tsh in clones that had ectopic JAK/STAT signaling.…”

Section: Resultsmentioning

confidence: 99%

JAK/STAT signaling is required for hinge growth and patterning in the Drosophila wing disc

Ayala-Camargo

Anderson

Amoyel

et al. 2013

Developmental Biology

View full text Add to dashboard Cite

SUMMARY JAK/STAT signaling is localized to the wing hinge, but its function is not known. Here we show that the Drosophila STAT Stat92E is downstream of Homothorax and is required for hinge development by cell-autonomously regulating hinge-specific factors. Within the hinge, Stat92E activity becomes restricted to gap domain cells that lack Nubbin and Teashirt. While gap domain cells lacking Stat92E have significantly reduced proliferation, increased JAK/STAT signaling there does not expand this domain. Thus, this pathway is necessary but not sufficient for gap domain growth. We show that reduced Wingless (Wg) signaling dominantly inhibits Stat92E activity in the hinge. However, ectopic JAK/STAT signaling does not perturbs Wg expression in the hinge. We report negative interactions between Stat92E and the notum factor Araucan, resulting in restriction of JAK/STAT signaling from the notum. In addition, we find that the distal factor Nub represses the ligand unpaired as well as Stat92E activity. These data suggest that distal expansion of JAK/STAT signaling is deleterious to wing blade development. Indeed, mis-expression of Unpaired within the presumptive wing blade causes small, stunted adult wings. We conclude that JAK/STAT signaling is critical for hinge fate specification and growth of the gap domain and that its restriction to the hinge is required for proper wing development.

show abstract

Section: Resultsmentioning

confidence: 99%

JAK/STAT signaling is required for hinge growth and patterning in the Drosophila wing disc

Ayala-Camargo

Anderson

Amoyel

et al. 2013

Developmental Biology

View full text Add to dashboard Cite

show abstract

“…Our analysis of the RDGN considered all transcriptional regulators that can induce ectopic eyes when misexpressed in the fly: Ey, Eya, So, Dac, Toy, Teashirt (Tsh), Tiptop (Tio), Eyegone (Eyg), Twin of eyegone (Toe), Optix, Distal antenna (Dan), and Distal antenna-related (Danr) (Bessa et al, 2009; Bonini et al, 1997; Curtiss et al, 2007; Czerny et al, 1999; Datta et al, 2009; Halder et al, 1995; Jang et al, 2003; Li et al, 2013; Pan and Rubin, 1998; Seimiya and Gehring, 2000; Shen and Mardon, 1997; Weasner et al, 2007; Yao et al, 2008). The scoring scheme reaffirmed Ey, Eya, and So as core RDGN members, consistent with the prevailing opinion in the field (Table 1, Figs.…”

Section: Defining the Members Of Master Regulatory Networkmentioning

confidence: 99%

Master regulators in development: Views from the Drosophila retinal determination and mammalian pluripotency gene networks

Davis

Rebay

2017

Developmental Biology

View full text Add to dashboard Cite

Among the mechanisms that steer cells to their correct fate during normal development, master regulatory networks are unique in their sufficiency to trigger a developmental program outside of its normal context. In this review we discuss the key features that underlie master regulatory potency during normal and ectopic development, focusing on two examples, the retinal determination gene network (RDGN) that directs eye development in the fruit fly and the pluripotency gene network (PGN) that maintains cell fate competency in the early mammalian embryo. In addition to the hierarchical transcriptional activation, extensive positive transcriptional feedback, and cooperative protein-protein interactions that enable master regulators to override competing cellular programs, recent evidence suggests that network topology must also be dynamic, with extensive rewiring of the interactions and feedback loops required to navigate the correct sequence of developmental transitions to reach a final fate. By synthesizing the in vivo evidence provided by the RDGN with the extensive mechanistic insight gleaned from the PGN, we highlight the unique regulatory capabilities that continual reorganization into new hierarchies confers on master control networks. We suggest that deeper understanding of such dynamics should be a priority, as accurate spatiotemporal remodeling of network topology will undoubtedly be essential for successful stem cell based therapeutic efforts.

show abstract

“…This conclusion is been based, in part, on two lines of evidence. First, the position of induced ectopic eyes often intersects with the TGFβ and Hedgehog (Hh) expression domain [2], [4]–[8], [10]–[13], [17]. Second, co-expression of Ey and TGFβ or Hh in the wing expands the domain of ectopic eye formation [14], [17].…”

Section: Introductionmentioning

confidence: 99%

Identification of Retinal Transformation Hot Spots in Developing Drosophila Epithelia

Salzer

Kumar

2010

PLoS ONE

View full text Add to dashboard Cite

BackgroundThe retinal determination (RD) network is an evolutionarily conserved regulatory circuit that governs early events in the development of eyes throughout the animal kingdom. Ectopic expression of many members of this network leads to the transformation of non-retinal epithelia into eye tissue. An often-overlooked observation is that only particular cell-populations within a handful of tissues are capable of having their primary developmental instructions superseded and overruled.Methodology/Preliminary FindingsHere we confirm that indeed, only a discrete number of cell populations within the imaginal discs that give rise to the head, antenna, legs, wings and halteres have the cellular plasticity to have their developmental fates altered. In contrast to previous reports, we find that all transformable cell populations do not lie within the TGFβ or Hedgehog signaling domains. Additionally neither signaling cascade alone is sufficient for non-retinal cell types to be converted into retinal tissue. The transformation “hot spots” that we have identified appear to coincide with several previously defined transdetermination “weak spots”, suggesting that ectopic eye formation is less the result of one network overriding the orders of another, as previously thought, but rather is the physical manifestation of redirecting cell populations of enormous cellular plasticity. We also demonstrate that the initiation of eye formation in non-retinal tissues occurs asynchronously compared to that of the normal eye suggesting that retinal development is not under the control of a global developmental clock.Conclusions/SignificanceWe conclude that the subregions of non-retinal tissues that are capable of supporting eye formation represent specialized cell-populations that have a different level of plasticity than other cells within these tissues and may be the founder cells of each tissue.

show abstract

Zinc‐finger paralogues tsh and tio are functionally equivalent during imaginal development in Drosophila and maintain their expression levels through auto‐ and cross‐negative feedback loops

Cited by 36 publications

References 38 publications

JAK/STAT signaling is required for hinge growth and patterning in the Drosophila wing disc

JAK/STAT signaling is required for hinge growth and patterning in the Drosophila wing disc

Master regulators in development: Views from the Drosophila retinal determination and mammalian pluripotency gene networks

Identification of Retinal Transformation Hot Spots in Developing Drosophila Epithelia

Contact Info

Product

Resources

About