Zinc(II) niflumato complex with neocuproine: Synthesis, crystal structure, characterization and cytotoxic effects on human endometrial cell lines

Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines

“…The hypochromic effect observed in samples from the cell treated with complex clearly indicate structural changes of the nucleic acids which are visibly more pronounced in case of 12Z cell line. Decrease in absorbance at 260 nm might be associated with both direct interaction of the complex with nucleic acids which was confirmed by our previous studies on isolated DNA samples 79 , as well as by affecting transcription factors 80 . Obtained results are also in line with the higher cytotoxicity of the complex against 12Z cell line which is reflected by more significant hypochromic effect of the absorbance in lysed cell nuclei sample isolated from the affected cells.…”

“…Based on results of xCELLigence RTCA analysis we have found that while the concentration of 1 μM Zn-Nif has no cytotoxic effect in any interval of action on studied cell lines from, the concentration of 10 μM has the desired cytotoxic effect on the treated 12Z cell culture but not on the treated hTERT cells after 24 h. In our study, we observed that the Zn-Nif inhibits proliferation, migration, and showed a selective cytotoxic effect which was manifested in a significant reduction of viable cells in 48 h by 69% in the 12Z cell line and by 29% in the hTERT cell line, respectively. These measurements show that hTERT cells are more resistant to the effect of [Zn( neo )( nif ) 2 ] compared to 12Z cells and also correlate with the theory that NSAIDs complexed with bio-metal are more effectively reduce proliferation than NSAID alone 79 .…”

“…Our previous measurements of cytotoxicity interpreted as IC50 values show (Table 2 ) that the studied complex has approximately 4.6-fold greater cytotoxic effect for rapidly proliferating 12Z cells compared to hTERT cells 79 . The cis -platin ( cis Pt) was selected as comparative compound to detect the efficiency of Zn-Nif complex.…”

“…Studied complex [Zn( neo )( nif ) 2 ] was prepared in crystalline according to the previously reported procedure 79 . The structure of the complex was verified by single crystal X-ray diffraction analysis as well as spectral methods (IR, UV–VIS, fluorescence).…”

In Vitro Cytotoxic Evaluation of a Silver(I) Complex Including Non-Steroidal Anti-Inflammatory Drug Niflumic Acid and 3-Picoline on Human-Derived Cancer Cell Lines

“…For instance, the cytotoxic activity of [Co(bcp)(nif)2] was scrunized on various cancer cell types, namely HeLa, HT-29, PC-3 (human prostate cancer), and MCF-7 cells, and the IC50 values belonging these cell lines were reported to be 38.75, 43.67, 27.06, and 41.53 µM, respectively [29]. In another study, the IC50 value of the [Zn(neo)(nif)2] complex on two endometrial (hTERT and 12Z) cell lines was reported to be 4.2 and 0.9 µM, respectively [30]. Moreover, in our previous study we tested the cytotoxicity of AgH(nif)2 complex against MCF-7, HT-29, and human hepatoma cells (HepG2) cancer cell lines, and the results indicated that the IC50 values of this complex on each cell line was 39.24, 71.02, and 58.38 µM, respectively [31].…”