Zinc in plasma, neutrophils, lymphocytes, and erythrocytes as determined by flameless atomic absorption spectrophotometry.

Abstract

Zinc is determined in neutrophils and lymphocytes (isolated from whole blood on discontinuous gradients of Ficoll-Hypaque) and in microliter quantities of plasma and erythrocytes by flameless atomic absorption spectrophotometry with greater sensitivity than with conventional flame atomic absorption spectrophotometry. Before analysis, neutrophils and lymphocytes are digested with nitric acid and diluted with de-ionized water. Plasma and erythrocytes required no digestion, only dilution. Overall CVs were 4.0, 3.0, 5.0, and 4.6% for neutrophils, lymphocytes, erythrocytes, and plasma, respectively. Matrix effects were fully compensated for by use of standard solutions that simulated the sample matrix. Results for plasma and erythrocytes agreed with those obtained by the conventional technique.

Rapid and cooperative binding of factor XII to human umbilical vein endothelial cells

“…A prerequisite for FXII binding to endothelial cells was an appropriate concentration of Zn 21 . The total concentration of Zn 21 in plasma is 10±25 mm [22]. The majority of this is bound primarily to albumin while the free Zn 21 concentration is significantly lower (0.2±0.5 mm) [23].…”

“…In vivo, an increase in the Zn 21 concentration may locally be granted by disturbed cells in the circulation. Neutrophils, lymphocytes [22] and platelets [24] contain high concentrations of Zn 21 and may be considered a Zn 21 pool to be released as a response to cellular stimulation. The role of FXII binding to the cells as a consequence of a local increase in Zn 21 concentration is at present not known.…”

“…During each passage the concentration of living cells for seeding was tested by trypan blue staining. For experiments the subcultured cryopreserved cells were quickly thawed and plated in tissue culture flasks at a concentration of 7.5 Â 10 3 cells´cm 22 . At confluence the cells were transferred to 12-well dishes at a concentration of (2±3) Â 10 4 cells´cm 22 .…”

Effect of zinc supplementation on oxidative drug metabolism in patients with hepatic cirrhosis.

“…Zinc status was represented by both serum and leucocyte zinc concentrations. Zinc levels were measured by atomic absorption spectrophotometry (Whitehouse et al, 1982). Fasting samples of blood taken with minimal stasis into trace element free Vacutainers were used.…”

Divalent metal binding by histidine‐rich glycoprotein differentially regulates higher order oligomerisation and proteolytic processing

“…ITC was then used to determine the affinities and stoichiometries of binding for each divalent metal to HRG. HRG showed the following order of affinity for Zn 2+ (K d : 2.85 lM) > Ni 2+ (K d : 3.35 lM) > Cu 2+ (K d : 3.72 lM) > Co 2+ (K d : 9.39 lM), with stoichiometries of Zn 2+ (9-10), Ni 2+ (13), Cu 2+ (6), and Co 2+ (13) ( Fig. 2A-D).…”