Zinc-induced cortical neuronal death with features of apoptosis and necrosis: Mediation by free radicals

Kim, Y.-H.; Kim, E.Y.; Gwag, Byoung Joo; Sohn, Seonghyang; Koh, Jae‐Young

doi:10.1016/s0306-4522(98)00313-3

Cited by 248 publications

(148 citation statements)

References 45 publications

Supporting

Mentioning

138

Contrasting

Unclassified

Order By: Relevance

“…Trolox is an analog of vitamin E, which protects lipid membranes from oxidation and has been shown to reduce ROS-mediated cell death in neurons and cardiomyocytes. 86,87 Ten micromolar Trolox treatment was sufficient to rescue cell viability as measured by ATP levels (Supplementary Fig. S1B) after exposure to nongelling methacrylate polymerizations, further suggesting that ROS generated from methacrylate polymerization causes decreased SMG cell viability.…”

Section: Resultsmentioning

confidence: 97%

Development of Poly(Ethylene Glycol) Hydrogels for Salivary Gland Tissue Engineering Applications

Shubin

Felong

Graunke

et al. 2015

Tissue Engineering Part A

View full text Add to dashboard Cite

More than 40,000 patients are diagnosed with head and neck cancers annually in the United States with the vast majority receiving radiation therapy. Salivary glands are irreparably damaged by radiation therapy resulting in xerostomia, which severely affects patient quality of life. Cell-based therapies have shown some promise in mouse models of radiation-induced xerostomia, but they suffer from insufficient and inconsistent gland regeneration and accompanying secretory function. To aid in the development of regenerative therapies, poly(ethylene glycol) hydrogels were investigated for the encapsulation of primary submandibular gland (SMG) cells for tissue engineering applications. Different methods of hydrogel formation and cell preparation were examined to identify cytocompatible encapsulation conditions for SMG cells. Cell viability was much higher after thiol-ene polymerizations compared with conventional methacrylate polymerizations due to reduced membrane peroxidation and intracellular reactive oxygen species formation. In addition, the formation of multicellular microspheres before encapsulation maximized cell-cell contacts and increased viability of SMG cells over 14-day culture periods. Thiol-ene hydrogel-encapsulated microspheres also promoted SMG proliferation. Lineage tracing was employed to determine the cellular composition of hydrogel-encapsulated microspheres using markers for acinar (Mist1) and duct (Keratin5) cells. Our findings indicate that both acinar and duct cell phenotypes are present throughout the 14 day culture period. However, the acinar:duct cell ratios are reduced over time, likely due to duct cell proliferation. Altogether, permissive encapsulation methods for primary SMG cells have been identified that promote cell viability, proliferation, and maintenance of differentiated salivary gland cell phenotypes, which allows for translation of this approach for salivary gland tissue engineering applications.

show abstract

Section: Resultsmentioning

confidence: 97%

Development of Poly(Ethylene Glycol) Hydrogels for Salivary Gland Tissue Engineering Applications

Shubin

Felong

Graunke

et al. 2015

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…Zinc, for example, can alter cellular signal transduction (1), DNA synthesis (2, 3), gene transcription (4,5), post transcriptional modifications (6), protein-protein interactions (7), and protein function (8,9). Increases or decreases in intracellular zinc have been associated with apoptotic cell death (10)(11)(12)(13). As the essential role of metals in cell biology is well recognized, there have been relatively few attempts to alter the biology of cancer cells by altering their metal content.…”

Section: Introductionmentioning

confidence: 99%

Metal ionophores – An emerging class of anticancer drugs

2009

View full text Add to dashboard Cite

SummaryCompounds that bind metals such as copper and zinc have many biological activities, including the ability to induce apoptosis in cancer cells. Although some of these compounds have been considered to act as chelators of metals, decreasing their bioavailability, others increase intracellular metal concentrations. We review recent work regarding the recognition of the biological effects of metal ionophores with different structures, particularly with regard to their actions upon cancer cells focusing on dithiocarbamates, pyrithione, and the 8-hydroxyquinoline derivative, clioquinol. We provide a biologically based classification of metal-binding compounds that allows an experimental distinction between chelators and ionophores that can be readily used by biologists, which may lead to further study and classification of metal-binding drugs. Metal ionophores may kill cancer cells by a number of mechanisms, including lysosomal disruption and proteasome inhibition, and likely others. Because some of these compounds have been safely administered to animals and humans, they have the potential to become clinically useful anticancer agents.

show abstract

“…The induction of apoptosis by high levels of intracellular zinc has been shown in different tissues and cell types (Truong-Tran et al 2001;Watjen et al 2002). Reports indicate that accumulation of intracellular zinc, as a consequence of either exogenous administration or release from intracellular stores by reactive oxygen species or nitrosation, activates pro-apoptotic molecules like p38 and potassium channels, leading to cell death (Kim et al 1999;McLaughlin et al 2001;TruongTran et al 2001). Increased intracellular zinc levels may also induce cell death by inhibition of the energy metabolism (Brown et al 2000;Sheline et al 2000).…”

Section: Discussionmentioning

confidence: 99%

“…In the context of its apoptosis-inducing properties, zinc has been shown to increase the expression of Bax, leading to a decrease in the Bcl-2/Bax ratio (Feng et al 2008). As a consequence, dissipation of the mitochondrial membrane potential leads to the release of cytochrome-c from mitochondria into the cytosol (Kim et al 1999;Bitanihirwe and Cunningham 2009). Zinc has an important role in gastric function and pathology.…”

Section: Discussionmentioning

confidence: 99%

Transient receptor potential melastatin type 7 channels are involved in zinc-induced apoptosis in gastric cancer

Kim¹

2011

Animal Cells and Systems

View full text Add to dashboard Cite

Zinc-induced cortical neuronal death with features of apoptosis and necrosis: Mediation by free radicals

Cited by 248 publications

References 45 publications

Development of Poly(Ethylene Glycol) Hydrogels for Salivary Gland Tissue Engineering Applications

Development of Poly(Ethylene Glycol) Hydrogels for Salivary Gland Tissue Engineering Applications

Metal ionophores – An emerging class of anticancer drugs

Transient receptor potential melastatin type 7 channels are involved in zinc-induced apoptosis in gastric cancer

Contact Info

Product

Resources

About