Zinc Inhibition of cAMP Signaling

Klein, Claudette; Sunahara, Roger K.; Hudson, Tracie Y.; Heyduk, Tomasz; Howlett, Allyn C.

doi:10.1074/jbc.m108808200

Cited by 64 publications

(56 citation statements)

References 30 publications

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“…Moreover, to preclude any effects of Cu 2ϩ or Zn 2ϩ on AC activity per se, after incubation of the G␣ s in the presence or absence of the metal cations, DTT (1 mM) was added. It has been shown that in the presence of DTT, Zn 2ϩ does not alter AC activity (22). Indeed, in our experiments also this treatment did not alter control activity of AC (Fig.…”

Section: Cusupporting

confidence: 55%

Section: ؉mentioning

confidence: 99%

“…2ϩ Than Forskolin-stimulated AC-Zn 2ϩ has been demonstrated to inhibit certain isoforms of AC, including ACVI (22). Because our studies demonstrate that Zn 2ϩ also inhibits G␣ s activation, we determined the relative sensitivity of receptor-mediated stimulation of AC versus direct inhibition of the enzyme by Zn 2ϩ .…”

Section: Receptor-mediated Activation Of Ac Is More Sensitive To Inhimentioning

confidence: 99%

“…Because cAMP plays a crucial role in the regulation of a number of cellular processes, one study has investigated the actions of Zn 2ϩ on ACs and shown that Zn 2ϩ inhibits the activity of type I, V, and VI ACs (22). The hormonal stimulation of cAMP was also attenuated by Zn 2ϩ (22). However, it is not clear whether and how Cu 2ϩ and Zn 2ϩ would influence the function of trimeric GTP-binding proteins that play an important role in regulating the activity of AC and synthesis of second messenger cAMP.…”

Section: ؉mentioning

confidence: 99%

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Copper and Zinc Inhibit Gαs Function

Gao¹,

Du²,

Patel

2005

Journal of Biological Chemistry

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؊ with the nucleotide-free G␣ s is sufficient to activate AC. Using antibodies to the N and C termini of G␣ s , we show that the Cu 2؉ interaction site on the G protein is in the C terminus. We conclude that Cu 2؉ and Zn 2؉ generate a nucleotide-free state of G␣ s and that, in the absence of any nucleotide, the ␥-phosphate mimic of GTP, AlF 4 ؊ , alters G␣ s structure sufficiently to permit stimulation of AC activity. Moreover, our finding that isoproterenolstimulated AC activity was more sensitive to inhibition by Cu 2؉ and Zn 2؉ as compared with forskolin-stimulated activity is consistent with G␣ s being a primary target of these cations in regulating the signaling from receptor to AC.

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Section: Cusupporting

confidence: 55%

Section: ؉mentioning

confidence: 99%

Section: Receptor-mediated Activation Of Ac Is More Sensitive To Inhimentioning

confidence: 99%

Section: ؉mentioning

confidence: 99%

See 2 more Smart Citations

Copper and Zinc Inhibit Gαs Function

Gao¹,

Du²,

Patel

2005

Journal of Biological Chemistry

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“…It has been reported that zinc deficiency lowers gestation and affects the nervous system, thus reducing appetite (Levenson, 2003). Many important enzymes and cytokines require zinc to function (Shankar and Prasad, 1998;Iskra and Majewski, 2000;McCall et al, 2000;Klein et al, 2002), and it is considered that zinc deficiency may adversely affect various vital functions and reduce metabolism and daily activity.…”

Section: Discussionmentioning

confidence: 99%

Ratio of low serum zinc levels in elderly Japanese people living in the central part of Japan

et al. 2006

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Objective: Elderly patients often have impaired taste, nausea, anorexia and delayed healing of decubitus. In many of these patients, serum zinc levels are low and they respond to zinc supplementation. To date, no epidemics of zinc deficiency have been reported in Japan. We studied the prevalence of zinc deficiency and its causative factors in a typical local town in Japan. Design: Cross-sectional study. Setting: Nagano Prefecture (central Japan). Subjects and methods: Serum zinc levels were measured in 1009 habitants (18-96 years old) who participated in an annual mass health examination program of the City in 2003. Of all subjects, 86 with low serum zinc (o65 mg/dl) or high zinc (X90 mg/ dl) levels were randomly selected, and a dietary survey using 24-h recalling methods for 2 consecutive days was performed in 2004. Among them, blood was collected in the morning from 50 subjects (26-94 years old). Results: The percentage of those with low serum zinc levels (2.5th percentile of Americans) was 37.9% in the elderly (X60 years old). The age-adjusted prevalence of low serum zinc was 21.1% in the aged population. A positive correlation was detected between zinc intake and serum zinc levels in elderly subjects (X60 years old). Conclusions: The possibility of zinc deficiency in adult inhabitants in central Japan rises with age. The deficiency correlates with dietary zinc intake. Sponsorship: This research was supported by a grant from local government officials in Tomi City (2004) and the funding of Fancl Corporation.

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Escherichia coli Bacteria Develop Adaptive Resistance to Antibacterial ZnO Nanoparticles

et al. 2018

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Antibacterial agents based on nanoparticles (NPs) have many important applications, e.g., for the textile industry, surface disinfection, wound dressing, water treatment, and food preservation. Because of their prevalent use it is important to understand whether bacteria could develop resistance to such antibacterial NPs similarly to the resistance that bacteria are known to develop to antibiotics. Here, it is reported that Escherichia coli (E. coli) develops adaptive resistance to antibacterial ZnO NPs after several days' exposure to the NPs. But, in contrast to antibiotics‐resistance, the observed resistance to ZnO NPs is not stable—after several days without exposure to the NPs, the bacteria regain their sensitivity to the NPs' antibacterial properties. Based on the analyses it is suggested that the observed resistance is caused by changes in the shape of the bacteria and the expressions of membrane proteins. The findings provide insights into the response of bacteria to antibacterial NPs, which is important to elucidate for designing and evaluating the risk of applications based on antibacterial NPs.

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Zinc Inhibition of cAMP Signaling

Cited by 64 publications

References 30 publications

Copper and Zinc Inhibit Gαs Function

Copper and Zinc Inhibit Gαs Function

Ratio of low serum zinc levels in elderly Japanese people living in the central part of Japan

Escherichia coli Bacteria Develop Adaptive Resistance to Antibacterial ZnO Nanoparticles

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