Zinc Ion-Based Switch-on Fluorescence-Sensing Probes for the Detection of Tetracycline

Abstract

Tetracycline (TC) is an antibiotic that has been widely used in the animal husbandry. Thus, TC residues may be found in animal products. Developing simple and sensitive methods for rapid screening of TC in complex samples is of great importance. Herein, we demonstrate a fluorescence-sensing method using Zn2+ as sensing probes for the detection of TC. Although TC can emit fluorescence under the excitation of ultraviolet light, its fluorescence is weak because of dynamic intramolecular rotations, leading to the dissipation of excitation energy. With the addition of Zn2+ prepared in tris(hydroxymethyl)amino-methane (Tris), TC can coordinate with Zn2+ in the Zn2+-Tris conjugates to form Tris-Zn2+-TC complexes. Therefore, the intramolecular motions of TC are restricted to reduce nonradiative decay, resulting in the enhancement of TC fluorescence. Aggregation-induced emission effects also play a role in the enhancement of TC fluorescence. Our results show that the linear dynamic range for the detection of TC is 15–300 nM. Moreover, the limit of detection was ~7 nM. The feasibility of using the developed method for determination of the concentration of TC in a complex chicken broth sample is also demonstrated in this work.

Novel Enzyme-Assisted Recycle Amplification Strategy for Tetracycline Detection Based on Oxidized Single-Walled Carbon Nanohorns

“…First, a 15 µL 100 nmol/L ssDNA solution was mixed with TC solution at varying concentrations (5,8,17,20,25,30, and 35 ng/mL) and incubated at 37 • C for 30 min. Second, 1.5 µL of 100 µg/mL oxSWCNHs was added and allowed to react at room temperature for 10 min.…”

Recent advances in aggregation-induced emission (AIE)-based chemosensors for the detection of organic small molecules

“…Chen et al reported RIM of TC in coordination with Zn 2+ -tris(hydroxymethyl)amino-methane conjugate ( B11 ) which causes the turn-on of green-yellow fluorescence. 188 In another example, Li and Pu et al prepared a ratiometric fluorescence probe based on AIE-active lumino-Eu 3+ complex ( B12 ), which changes fluorescence colour from blue to red when TC was added in the presence of citrate as a chelating agent. 189 This quenching mechanism is attributed to IFE caused by TC which quenches 453 nm emission of lumino-Eu 3+ complex at the expense of 626 nm Eu 3+ emission.…”

A Highly Selective and Sensitive Sequential Recognition Probe Zn2+ and H2PO4− Based on Chiral Thiourea Schiff Base

“…In recent years, widespread research has been undertaken on the detection of metal ions [ 1 , 2 , 3 , 4 , 5 ] and inorganic anions [ 6 , 7 , 8 , 9 ], particularly within the development of selective ion probes, which has more generally become a popular topic in scientific research. Zn 2+ is the second most common element in the human body, after iron, and not only plays an important role in protein structure, catalysis, transcription, and regulation, but is also closely associated with nerve signal transmission, enzyme regulation, and gene expression [ 10 ].…”