Zinc kinetics in patients with bone demineralization due to physical immobilization.

Higashi, Akimasa; Nakamura, Teruya; Nishiyama, Soroku; Matsukura, Makoto; Tomoeda, Shinichi; Y, Futagoishi; Shinohara, M; Matsuda, Ichiro

doi:10.1080/07315724.1993.10718284

Cited by 23 publications

(5 citation statements)

References 24 publications

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“…Finally, bone substitutes or coating layers containing ZnTCP would be promising biomaterials, because they counteract bone resorption at the bone‐implant interface. Aging, skeletal unloading, and immobilization lead to decreases in cellular and tissue Zn 2+ contents in bones 46–48. A decrease in Zn 2+ content is related to osteoporotic bone resorption in humans 49.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effect of Zn²⁺ in zinc‐containing β‐tricalcium phosphate on resorbing activity of mature osteoclasts

Yamada

Ito

Kojima

et al. 2007

J Biomedical Materials Res

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Long term effect of the growing instability of the bone-implant interface due to bone resorption at the interface is a problem for the implants, including bioactive ceramics. Zn2+ -containing tricalcium phosphate (ZnTCP) is a material which may overcome this problem. The present study aims to clarify whether Zn2+ -containing tricalcium phosphate (ZnTCP) ceramics with a Zn2+ content of 0.316 (ZnTCP316) and 0.633 (ZnTCP633) wt % suppress resorption by mature osteoclasts in vitro. Suppression would be due to an increase in the number of apoptotic osteoclasts and the inhibition of the resorbing activity of osteoclasts, the latter being the major mechanism of the suppression. The number of apoptotic osteoclasts was significantly 6.3 times higher with ZnTCP633 than with tricalcium phosphate ceramic (TCP) after 24-h culture. The net contribution to resorption of this change in apoptotic cell numbers is much smaller than that of the change in resorbing activity. The osteoclasts cultured on ZnTCP formed fewer actin rings than those cultured on the TCP. The mRNA expression of CAII and cathepsin K/OC2 in the osteoclasts on ZnTCP633 was downregulated 0.5-fold and 0.6-fold, respectively, compared with that on the TCP. The volume of resorption pits was downregulated 0.4-fold in the ZnTCP633 than that in TCP. These results suggest that ZnTCPs suppressed the resorbing activity of mature osteoclasts probably through a local increase in the level of Zn2+. Bone substitutes or coating layers containing ZnTCP would be promising biomaterials from the viewpoint of counteracting osteoclastic bone resorption at the bone-implant interface.

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Section: Discussionmentioning

confidence: 99%

Inhibitory effect of Zn²⁺ in zinc‐containing β‐tricalcium phosphate on resorbing activity of mature osteoclasts

Yamada

Ito

Kojima

et al. 2007

J Biomedical Materials Res

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“…Woman with osteoporosis excretes a great amount of zinc in urine [Herzberg et al, 1990]. Zinc supplementation has been shown to have preventive and therapeutic effects on bone loss [Higashi et al, 1993; Kishi et al, 1994]. Zinc may have a role as a nutritional and pharmacologic tools in the prevention of osteoporosis with increasing age [Yamaguchi, 1998; Yamaguchi and Uchiyama, 2003].…”

mentioning

confidence: 99%

Genistein and zinc synergistically stimulate apoptotic cell death and suppress RANKL signaling‐related gene expression in osteoclastic cells

Uchiyama

Yamaguchi

2007

J of Cellular Biochemistry

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The effects of the combination of genistein and zinc, which have an anabolic effect on bone metabolism, on osteoclastic cells in mouse marrow culture system in vitro was investigated. The macrophage colony-stimulating factor (M-CSF)-dependent bone marrow macrophages were cultured in the presence of M-CSF (10 ng/ml) and receptor activator of nuclear factor kappaB (NF-kB) ligand (RANKL; 50 ng/ml) for 4 days. The osteoclastic cells formed were further cultured in medium containing either vehicle, genistein, zinc sulfate (zinc), or genistein plus zinc with or without M-CSF (10 ng/ml) and RANKL (50 ng/ml) for 24 or 72 h. The number of osteoclastic cells was significantly decreased with culture of genistein (10(-6) M) plus zinc (10(-5) M) in presence or absence of M-CSF and RANKL for 24 or 72 h as compared with the value for genistein or zinc alone. Agarose gel electrophoresis showed the presence of low-molecular weight deoxyribonucleic acid (DNA) fragments of adherent cells cultured with genistein (10(-6) M) plus zinc (10(-5) M) for 24 or 72 h, indicating that the combination of two chemicals induces apoptotic cell death. Such an effect was not seen in the case of each chemical. Genistein plus zinc-induced decrease in osteoclastic cells were significantly inhibited in the presence of caspase-3 inhibitor (10(-8) or 10(-7) M). Culture with genistein (10(-6) M) plus zinc (10(-5) M) for 72 h caused a significant increase in caspase-3 mRNA expression in the presence or absence of M-CSF and RANKL as compared with the value for each chemical alone. Genistein plus zinc-induced increase in caspase-3 mRNA expression was completely inhibited in the presence of cycloheximide (10(-7) M), an inhibitor of protein synthesis, or 5, 6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB; 10(-6) M), an inhibitor of transcription activity. The mRNA expression of tartrate-resistant acid phosphatase (TRACP) or cathepsin K was significantly decreased with culture of genistein plus zinc in the presence of M-CSF and RANKL for 72 h as compared with genistein or zinc alone. Nuclear factor of activated T cells c1 (NFATc1) mRNA expression was significantly decreased with culture of genistein plus zinc in the presence of M-CSF and RANKL for 24 or 72 h as compared with each chemical alone, while NF-kB mRNA expression was significantly changed. This study demonstrates that the combination of genistein and zinc has potent stimulatory effects on apoptotic cell death and suppressive effects on osteoclastic cell function.

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“…Some researchers reported an association between reduced ADL and changes in biochemical results, including serum albumin level . In a study of 262 geriatric patients, 80% with lower ADL showed hypoalbuminaemia .…”

Section: Discussionmentioning

confidence: 99%

Activities of daily living are associated with outcomes of epilepsy treatment in elderly patients

et al. 2019

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Aim: Controlling epileptic seizures in elderly populations is widely considered to be relatively easy, but we hypothesized that the lifestyles of elderly individuals may affect the outcomes of epilepsy treatment. The purpose of this study was to review the activities of daily living (ADL) of elderly individuals with epilepsy and compare them with the outcomes of epilepsy treatment. Methods: Of the 177 patients ≥65 years old who were referred to our epilepsy centre, epilepsy was diagnosed in 84. ADL and treatment outcomes were then reviewed, with ADL classified into three levels: ADL I, without disability; ADL II, disabled only in some instrumental ADL; and ADL III, disabled in some basic ADL. Epilepsy syndromes and use of anti-seizure drugs were also evaluated. Results: Forty-five patients (53.6%) achieved freedom from seizures, 23 (27.4%) achieved ≥80% but <100% reduction in seizures, 5 (6%) achieved ≥50% but <80% reduction in seizures, and 11 (13.1%) achieved <50% reduction in seizures. Thirty-five patients (81.4%) with ADL I achieved freedom from seizures, compared with seven patients with ADL II (28.0%) and three patients with ADL III (19.0%). A significant difference was evident among the three groups (F = 6.145, P = 0.003). Conclusions: ADL should be taken into account when an epilepsy treatment is being selected.

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Zinc kinetics in patients with bone demineralization due to physical immobilization.

Cited by 23 publications

References 24 publications

Inhibitory effect of Zn²⁺ in zinc‐containing β‐tricalcium phosphate on resorbing activity of mature osteoclasts

Inhibitory effect of Zn²⁺ in zinc‐containing β‐tricalcium phosphate on resorbing activity of mature osteoclasts

Genistein and zinc synergistically stimulate apoptotic cell death and suppress RANKL signaling‐related gene expression in osteoclastic cells

Activities of daily living are associated with outcomes of epilepsy treatment in elderly patients

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Zinc kinetics in patients with bone demineralization due to physical immobilization.

Cited by 23 publications

References 24 publications

Inhibitory effect of Zn2+ in zinc‐containing β‐tricalcium phosphate on resorbing activity of mature osteoclasts

Inhibitory effect of Zn2+ in zinc‐containing β‐tricalcium phosphate on resorbing activity of mature osteoclasts

Genistein and zinc synergistically stimulate apoptotic cell death and suppress RANKL signaling‐related gene expression in osteoclastic cells

Activities of daily living are associated with outcomes of epilepsy treatment in elderly patients

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Inhibitory effect of Zn²⁺ in zinc‐containing β‐tricalcium phosphate on resorbing activity of mature osteoclasts

Inhibitory effect of Zn²⁺ in zinc‐containing β‐tricalcium phosphate on resorbing activity of mature osteoclasts