Zinc oxide-gold nanocomposite as a proper platform for label-free DNA biosensor

Hatami, Zahra; Ragheb, Elham; Jalali, Fahimeh; Tabrizi, Mahmoud Amouzadeh; Shamsipur, Mojtaba

doi:10.1016/j.bioelechem.2020.107458

Cited by 39 publications

(9 citation statements)

References 57 publications

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“…15,16 Moreover, specic functional groups, for binding of different molecules, have been formed on the surface of ZnO-based nanocomposites, opening a broad range of applications, especially in biosensors. 10,[19][20][21] The polymerization of functional polymers (polypyrrole, polyaniline, etc.) on ZnO surfaces requires special conditions, such as a low pH (2-3).…”

Section: Introductionmentioning

confidence: 99%

Synthesis and photoluminescence properties of hybrid 1D core–shell structured nanocomposites based on ZnO/polydopamine

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Synthesis and photoluminescence properties of hybrid 1D core–shell structured nanocomposites based on ZnO/polydopamine

et al. 2020

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“…they quantitatively monitor the electrical signals generated by the binding between antibodies and target molecules or antigens of M. tuberculosis . Moreover, the current nanomaterial immunosensor relies on the specific chemical, physical and electronic properties of the nanomaterials themselves to improve the performance of the sensing device and diagnose TB in real-time ( Mohd Bakhori et al, 2019 ; Hatami et al, 2020 ; Kahng et al, 2020 ). Although biosensors are promising, portable, easy to operate, with no amplification steps, immunosensors did not achieve the same success as immunoassays( Shin et al, 2015 ).…”

Section: New Promising Diagnostic Techniquesmentioning

confidence: 99%

Improved Conventional and New Approaches in the Diagnosis of Tuberculosis

Dong

et al. 2022

Front. Microbiol.

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Tuberculosis (TB) is a life-threatening infectious disease caused by Mycobacterium tuberculosis (M. tuberculosis). Timely diagnosis and effective treatment are essential in the control of TB. Conventional smear microscopy still has low sensitivity and is unable to reveal the drug resistance of this bacterium. The traditional culture-based diagnosis is time-consuming, since usually the results are available after 3–4 weeks. Molecular biology methods fail to differentiate live from dead M. tuberculosis, while diagnostic immunology methods fail to distinguish active from latent TB. In view of these limitations of the existing detection techniques, in addition to the continuous emergence of multidrug-resistant and extensively drug-resistant TB, in recent years there has been an increase in the demand for simple, rapid, accurate and economical point-of-care approaches. This review describes the development, evaluation, and implementation of conventional diagnostic methods for TB and the rapid new approaches for the detection of M. tuberculosis.

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“…Also, carboxyl functional groups existing on graphene form covalent amide bonding with the amine group of antibodies upon their surface deposition, creating more stable bonds and increasing the stability and sensitivity of the immunosensors. To this end, the accompanying elements such as gold nanoparticles have been used to hybridize DNA probe strands on ZnO–Gr nanocomposites to connect the cross-linkers needed for immobilizing the carboxylic groups. In another study, for creating hydrogen peroxide sensors, pyrene succinimide ester (PSE) was utilized to functionalize ZnO–Gr nanocomposites to immobilize streptavidin .…”

Section: Introductionmentioning

confidence: 99%

Highly Stable Buffer-Based Zinc Oxide/Reduced Graphene Oxide Nanosurface Chemistry for Rapid Immunosensing of SARS-CoV-2 Antigens

Haghayegh

Salahandish

Hassani

et al. 2022

ACS Appl. Mater. Interfaces

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The widespread and long-lasting effect of the COVID-19 pandemic has called attention to the significance of technological advances in the rapid diagnosis of SARS-CoV-2 virus. This study reports the use of a highly stable buffer-based zinc oxide/reduced graphene oxide (bbZnO/rGO) nanocomposite coated on carbon screen-printed electrodes for electrochemical immuno-biosensing of SARS-CoV-2 nuelocapsid (N-) protein antigens in spiked and clinical samples. The incorporation of a salt-based (ionic) matrix for uniform dispersion of the nanomixture eliminates multistep nanomaterial synthesis on the surface of the electrode and enables a stable single-step sensor nanocoating. The immuno-biosensor provides a limit of detection of 21 fg/mL over a linear range of 1–10 000 pg/mL and exhibits a sensitivity of 32.07 ohms·mL/pg·mm2 for detection of N-protein in spiked samples. The N-protein biosensor is successful in discriminating positive and negative clinical samples within 15 min, demonstrating its proof of concept used as a COVID-19 rapid antigen test.

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Zinc oxide-gold nanocomposite as a proper platform for label-free DNA biosensor

Cited by 39 publications

References 57 publications

Synthesis and photoluminescence properties of hybrid 1D core–shell structured nanocomposites based on ZnO/polydopamine

Synthesis and photoluminescence properties of hybrid 1D core–shell structured nanocomposites based on ZnO/polydopamine

Improved Conventional and New Approaches in the Diagnosis of Tuberculosis

Highly Stable Buffer-Based Zinc Oxide/Reduced Graphene Oxide Nanosurface Chemistry for Rapid Immunosensing of SARS-CoV-2 Antigens

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