Zinc Oxide Nanoparticles Induce Cell Filamentation in <i>Escherichia coli</i>

Gunawan, Cindy; Teoh, Wey Yang; ricardo,; Marquis, Christopher P.; Amal, Rose

doi:10.1002/ppsc.201200152

Cited by 13 publications

(8 citation statements)

References 30 publications

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“…Small clusters of cells, which did not aggregate to form complex biofilm structures (panel C), or multilayered cell clusters (panels E and F), were observed to be patchily distributed on the surface of ZnO35 pellets instead of multi-layered three-dimensional structures formed on G1 pellets, after 24 h of biofilm development. As shown in panel 6E, zinc oxide induced cell filamentation in E. coli confers certain survival advantages as previously reported by Gunawan et al [ 32 ]. Images after 5 days revealed sparsely colonized surfaces by microorganisms (panels D and G), abundant cellular debris, and seriously damaged cellular structures (panels D and H).…”

Section: Resultssupporting

confidence: 84%

Effect of the Medium Composition on the Zn2+ Lixiviation and the Antifouling Properties of a Glass with a High ZnO Content

et al. 2017

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The dissolution of an antimicrobial ZnO-glass in the form of powder and in the form of sintered pellets were studied in water, artificial seawater, biological complex media such as common bacterial/yeast growth media (Luria Bertani (LB), yeast extract, tryptone), and human serum. It has been established that the media containing amino acids and proteins produce a high lixiviation of Zn2+ from the glass due to the ability of zinc and zinc oxide to react with amino acids and proteins to form complex organic compounds. The process of Zn2+ lixiviation from the glass network has been studied by X-ray photoelectron spectroscopy (XPS). From these results we can state that the process of lixiviation of Zn2+ from the glass network is similar to the one observed in sodalime glasses, where Na+ is lixiviated to the media first and the fraction of Zn that acts as modifiers (~2/3) is lixiviated in second place. After the subsequent collapse of the outer surface glass layer (about 200–300 nm thick layer) the dissolution process starts again. Antifouling properties against different bacteria (S. epidermidis, S. aureus, P. aeruginosa, E. coli, and M. lutea) have also been established for the glass pellets.

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Section: Resultssupporting

confidence: 84%

Effect of the Medium Composition on the Zn2+ Lixiviation and the Antifouling Properties of a Glass with a High ZnO Content

et al. 2017

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“…The findings also put into question the common use of silver salt as model material for evaluating bacterial toxicity of Ag NPs. The rapid development in nanotechnology has seen inorganic nanomaterials such as nanosilver, 59 copper oxide and zinc oxide, subjected to advanced physicochemical manipulation to exhibit 60 powerful antimicrobial activity (Gunawan et al 2009, 2013a, Hajipour et al 2012. 61 ions.…”

mentioning

confidence: 99%

“…in nanotechnology has seen inorganic nanomaterials such as nanosilver, 59 copper oxide and zinc oxide, subjected to advanced physicochemical manipulation to exhibit 60 powerful antimicrobial activity(Gunawan et al 2009, 2013a, Hajipour et al 2012. 61Among these materials, nanosilver (silver nanoparticles, Ag NPs) is currently one of the most 62 commercialized due to its potent and broad-spectrum antimicrobial characteristics (Consumer 63Products Inventory -Project on Emerging Nanotechnologies).…”

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confidence: 99%

Nanosilver and the microbiological activity of the particulate solids versus the leached soluble silver

et al. 2018

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Nanosilver (Ag NPs) is currently one of the most commercialized antimicrobial nanoparticles with as yet, still unresolved cytotoxicity origins. To date, research efforts have mostly described the antimicrobial contribution from the leaching of soluble silver, while the undissolved solid Ag particulates are often considered as being microbiologically inert, serving only as source of the cytotoxic Ag ions. Here, we show the rapid stimulation of lethal cellular oxidative stress in bacteria by the presence of the undissolved Ag particulates. The cytotoxicity characteristics are distinct from those arising from the leached soluble Ag, the latter being locked in organic complexes. The work also highlights the unique oxidative stress-independent bacterial toxicity of silver salt. Taken together, the findings advocate that future enquiries on the antimicrobial potency and also importantly, the environmental and clinical impact of Ag NPs use, should pay attention to the potential bacterial toxicological responses to the undissolved Ag particulates, rather than just to the leaching of soluble silver. The findings also put into question the common use of silver salt as model material for evaluating bacterial toxicity of Ag NPs.

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“…The release of engineered nanoparticles into the environment leads to the contact of these particles with soil bacteria. This contact can result in inhibition of bacterial growth with the inhibitory effect increasing with decreasing agglomerate size [5][6][7][8][9]. The assessment of growth inhibition due to nanoparticle-specific characteristics of metal oxide nanoparticles requires the dispersion and stabilization of these particles in aqueous, pH neutral, ion-and protein-containing growth media such as lysogeny broth (LB) medium.…”

Section: Introductionmentioning

confidence: 99%

Zinc Oxide Nanoparticles in Bacterial Growth Medium: Optimized Dispersion and Growth Inhibition of <i>Pseudomonas putida</i>

Vielkind¹,

Kampen²,

Kwade³

2013

ANP

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The majority of nanoparticles tend to agglomerate in bacterial growth media. Thus, nanoparticle-specific characteristics can get lost. To investigate the influence of nanoparticles on bacteria, these particles should remain in their nanoparticulate state. The present study demonstrates the stabilization of commercially available zinc oxide (ZnO) with sodiumhexametaphosphate (SHMP) in bacterial growth medium (LB) to avoid agglomeration of these particles after the addition to LB. This established method is appropriate to stabilize ZnO agglomerates as small as 43 nm. The method of fractionated centrifugation was used to obtain stable agglomerates (also stable in the presence of bacteria) with different mean diameters. The SHMP-stabilized ZnO inhibits the growth of Pseudomonas putida with increasing concentration (up to 500 mg/L) and decreasing agglomerate size (43-450 nm).

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Zinc Oxide Nanoparticles Induce Cell Filamentation in Escherichia coli

Cited by 13 publications

References 30 publications

Effect of the Medium Composition on the Zn2+ Lixiviation and the Antifouling Properties of a Glass with a High ZnO Content

Effect of the Medium Composition on the Zn2+ Lixiviation and the Antifouling Properties of a Glass with a High ZnO Content

Nanosilver and the microbiological activity of the particulate solids versus the leached soluble silver

Zinc Oxide Nanoparticles in Bacterial Growth Medium: Optimized Dispersion and Growth Inhibition of <i>Pseudomonas putida</i>

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Zinc Oxide Nanoparticles Induce Cell Filamentation in Escherichia coli

Cited by 13 publications

References 30 publications

Effect of the Medium Composition on the Zn2+ Lixiviation and the Antifouling Properties of a Glass with a High ZnO Content

Effect of the Medium Composition on the Zn2+ Lixiviation and the Antifouling Properties of a Glass with a High ZnO Content

Nanosilver and the microbiological activity of the particulate solids versus the leached soluble silver

Zinc Oxide Nanoparticles in Bacterial Growth Medium: Optimized Dispersion and Growth Inhibition of &lt;i&gt;Pseudomonas putida&lt;/i&gt;

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Zinc Oxide Nanoparticles in Bacterial Growth Medium: Optimized Dispersion and Growth Inhibition of <i>Pseudomonas putida</i>