Zinc oxide nanowires-based electrochemical biosensor for L-lactic acid amperometric detection

Zhao, Yanguang; Yan, Xiaoqin; Kang, Zhuo; Fang, Xiaofei; Zheng, Xin; Zhao, Li; Du, Hongwu; Zhang, Yue

doi:10.1007/s11051-014-2398-y

Cited by 29 publications

(11 citation statements)

References 37 publications

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“…The analytical performance of the designed Pt/CeO2-TiO2/LOx/Nf biosensors were compared with already reported lactate biosensors, and the results are listed in Table 1. The table shows that the use of CeO2-TiO2 mixed metal oxide nanoparticles with large surface area and high catalytic activity enabled the construction of electrochemical lactate biosensors with higher sensitivity and lower limit of detection compared to some of the other works (31)(32)(33)(34). The novel sensor design reported in this work exhibited one of the highest sensitivity values among all.…”

Section: Electrochemical Performance Of the Constructed Electrochemical Lactate Biosensorsmentioning

confidence: 79%

The Use of CeO2-TiO2 Nanocomposites as Enzyme Immobilization Platforms in Electrochemical Sensors

Uzunoğlu

2017

Journal of the Turkish Chemical Society, Section A: Chemistry

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The use of metal oxide-based nanoparticles plays a key role in the development of electrochemical sensors with superior properties such as high sensitivity, wide linear range, low limit of detection, and extended storage stability. In this work, we aimed to synthesize CeO2-TiO2 mixed metal oxide nanoparticles which were used as substrate materials for the immobilization of bio-recognition element for the construction of enzyme-based electrochemical sensors. For this purpose, in the first part of the study, CeO2-TiO2 nanoparticles were prepared via a low-temperature co-precipitation method and characterized using X-ray Diffraction (XRD), N2-adsorption, and Transmission Electron Microscopy (TEM) methods. The XRD results confirmed the successful synthesis of CeO2-TiO2 mixed metal oxide nanoparticles with the average crystallite size of 8.51 nm. The calculated crystallite size value was compatible with that obtained from the TEM images. The N2 adsorption results revealed a large surface area of 78.6 cm 2 g-1 which is essential for the construction of electrochemical sensors with improved performance. The electrochemical sensors were developed by the deposition of nanoparticles on the surface of a Pt electrode, followed by the immobilization of lactate oxide enzyme. The electrochemical performance of the sensors was evaluated by cyclic voltammetry (CV) and chronoamperometry methods. The constructed sensors showed a sensitivity of 0.085 ± 0.008 µA µM-1 cm-2 (n=5) with a high reproducibility (RSD % = 1.3) and a wide linear range (0.02-0.6 mM). In addition, the detection limit towards lactate was found be 5.9 µM. The results indicated that the use of CeO2-TiO2 nanoparticles used as a modifier on the surface of the Pt electrode enabled the construction of electrochemical lactate sensors with high sensitivity.

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Section: Electrochemical Performance Of the Constructed Electrochemical Lactate Biosensorsmentioning

confidence: 79%

The Use of CeO2-TiO2 Nanocomposites as Enzyme Immobilization Platforms in Electrochemical Sensors

Uzunoğlu

2017

Journal of the Turkish Chemical Society, Section A: Chemistry

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“…; NR HRP (Type II from horseredish ) Chitosan/MWCNT/ferrocyanide/ gold electrode Amperomertic; NR NR 1.66 μM 5–340 μM Real food samples and beverages; NR 15 NR [80] LOD (no EC given);NR ZnO nanotetrapods Electrochemical; electrostatic adsorption 28.0 μA cm −2 mM −1 1.2 Μm 3.6 μM–0.6 mM NR; NR NR NR [120] LDH; (EC 1.1.1.27) rabbit’s muscle; 140 U mg −1 Fe 3 O 4 /MWCNT/GCE Electrochemical; covalent 7.67 µA mM −1 5 µM 50-500 µM Human serum sample; NR NR 14 [109] LDH (no EC given); rabbit's muscle; NR Nano-CeO 2 /GCE electrode Amperometric; NR NR 50 μM 200–2000 µM Blood samples; NR 4 NR [116] LOD(no EC given); NR; NR TiO 2 -NPs/PRG/GCE NR; NR 6.0 μA mM −1 0.6 Μm 2.0 μM–0.40 mM NR; NR NR NR [115] LOD (no EC given); Pediococcus sp. ; NR ZNO nanowires Amperometric; NR 15.6 µA cm −2 mM −1 12 μM 12–1200 μM NR; NR NR NR [119] L -LDH (no EC given); rabbit muscle; 130 U/mg Au/Nano ZnO electrode Electrochemical; covalent 1.832 µA µmol −1 L 4.73 nmol L −1 0.2–0.8 µmol L −1 Food products; urea 1 23 [132] …”

Section: Performance Factorsmentioning

confidence: 99%

“…Nanoparticles, such as multi walled carbon nanotubes (MWCNT)/Meldola blue [66] , MWCNT/poly (3-methylthiophene) (P3 MT) polymer modified glassy carbon electrode (GCE) [35] , nitrogen-doped CNT [34] , ferrocene CNT/polysulfon screen printed electrode [74] , pTTCA/MWNT composite film/Au electrode [94] , MWCNT/GCE [96] , Fe 3 O 4 /MWCNT/GCE [109] , MWCNT/CHIT composite/GCE [110] , SWCNTSs/Variamine blue [106] , Chitosan/MWCNT/ferrocyanide/gold electrode [80] , Chitosan/poly-vinyl imidazole Os (PVI-Os)/CNT [81] , MWCNTs/Pt-nano electrode [39] , gold nanoparticles (GNPs)/sol–gel 3-D silicate network derived from 3-(mercaptopropyl)trimethoxysilane (MPTS) [37] , gold nanorods [111] , platinum nanoparticles (Pt-black Nanoparticles) with gold electrode [112] , sol–gel film/MWCNT/Pt nanoparticle [97] , Polyacrylic acid (PAA)/Si 3 N 4 nanostructured [113] , Hydrogen titanate (H 2 Ti 3 O 7 ) nanotube network [114] , nanoparticles of metal oxides such as niobium oxide/carbon paste electrode [40] , Titanium dioxide nanoparticles (TiO 2 -NPs)/photocatalytically reduced graphene (PRG)/GCE [115] , Cerium oxide Nano-CeO 2 /GCE electrode [116] , molybdenum oxide nanowires, MoO 3 /Au coated SiO 2 electrode [41] and nanoparticles of semiconducting materials such as zinc oxide(ZNO)/MWCNT [42] , ZNO nanorod/glass coated substrate [117] , nano-ZnO-MWCNTs/GCE [118] , Au/Nano ZnO electrode [132] , ZNO nanowires [119] , ZnO nanotetrapods [120] .…”

Section: Classification Of L -Lactate Biosensorsmentioning

confidence: 99%

Biosensors based on electrochemical lactate detection: A comprehensive review

Rathee

Dhull

et al. 2016

Biochemistry and Biophysics Reports

226

197

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Lactate detection plays a significant role in healthcare, food industries and is specially necessitated in conditions like hemorrhage, respiratory failure, hepatic disease, sepsis and tissue hypoxia. Conventional methods for lactate determination are not accurate and fast so this accelerated the need of sensitive biosensors for high-throughput screening of lactate in different samples. This review focuses on applications and developments of various electrochemical biosensors based on lactate detection as lactate being essential metabolite in anaerobic metabolic pathway. A comparative study to summarize the L-lactate biosensors on the basis of different analytical properties in terms of fabrication, sensitivity, detection limit, linearity, response time and storage stability has been done. It also addresses the merits and demerits of current enzyme based lactate biosensors. Lactate biosensors are of two main types – lactate oxidase (LOD) and lactate dehydrogenase (LDH) based. Different supports tried for manufacturing lactate biosensors include membranes, polymeric matrices-conducting or non-conducting, transparent gel matrix, hydrogel supports, screen printed electrodes and nanoparticles. All the examples in these support categories have been aptly discussed. Finally this review encompasses the conclusion and future emerging prospects of lactate sensors.

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“…Also, H 2 O 2 is produced in many enzymatic reactions, which enables the detection of the chemical species involved in them [5][6][7]. Although there are various analytical techniques used for the detection of H 2 O 2 including photometry, chemiluminescence, chromatography, titrimetry, colorimetry, an alternative, simpler, detection method is still to be designed [8].…”

Section: Introductionmentioning

confidence: 99%

Bimetallic PdCu/SPCE non-enzymatic hydrogen peroxide sensors

Uzunoğlu

Scherbarth

Stanciu

2015

Sensors and Actuators B: Chemical

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a b s t r a c tScreen printed carbon electrodes (SPCE) were decorated with PdCu bimetallic alloys via a facile coelectrodeposition method to develop disposable non-enzymatic H 2 O 2 sensors. The electrochemical performance of the biosensors was evaluated in terms of selectivity, sensitivity, and stability with a goal of demonstrating that employing a bimetallic PdCu non-enzymatic system can push forward the state of the art in hydrogen peroxide sensing. The physical characterization of the biosensors was conducted using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and X-ray diffraction (XRD) techniques. Sensors consisting of PdCu bimetals showed higher sensitivity than Pd/SPCE and Cu/SPCE electrodes toward H 2 O 2 . The fabricated PdCu/SPCE sensors showed a sensitivity of 396.7 A mM −1 cm −2 , a linear range from 0.5 mM to 11 mM, and a low limit of detection (0.7 M) at the applied potential of −0.3 V. The use of relatively low working potential eliminated the interference effect of the common electroactive species (ascorbic acid, uric acid, and glucose) present in a real sample, which are usually a concern for non-enzymatic sensing systems. In addition, the high reproducibility (RSD = 2.5%), and excellent long term stability render PdCu/SPCEs as attractive materials for the construction of disposable enzyme-free H 2 O 2 sensors.

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Zinc oxide nanowires-based electrochemical biosensor for L-lactic acid amperometric detection

Cited by 29 publications

References 37 publications

The Use of CeO2-TiO2 Nanocomposites as Enzyme Immobilization Platforms in Electrochemical Sensors

The Use of CeO2-TiO2 Nanocomposites as Enzyme Immobilization Platforms in Electrochemical Sensors

Biosensors based on electrochemical lactate detection: A comprehensive review

Bimetallic PdCu/SPCE non-enzymatic hydrogen peroxide sensors

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