Zinc, Plasma Androgens and Male Sterility

Hartoma, T.Riita; Nahoul, K; Netter, A

doi:10.1016/s0140-6736(77)90563-3

Cited by 70 publications

(36 citation statements)

References 10 publications

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“…The results of the present study are in close agreement in man [47] and the rabbit [33]. However, no significant difference in testosterone levels was observed by Zn supplementation in the rabbit [25], man [34] and crossbred bulls [48], which may be due to species variation, different duration and level of zinc supplementation.…”

Section: Semen Qualitative Characteristics Of Crossbred Cattle Bulls supporting

confidence: 79%

Effect of different levels and sources of zinc supplementation on quantitative and qualitative semen attributes and serum testosterone level in crossbred cattle (Bos indicus×Bos taurus) bulls

et al. 2006

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-An experiment was conducted on 16 crossbred bulls (about 2 years of age, 316.2 ± 0.77 kg average body weight), divided into groups I, II, III and IV to study the effect of different levels of Zn supplementation from inorganic and organic sources on semen quality. The animals in the first 3 groups were supplemented with 0, 35 and 70 ppm Zn from Zn sulfate, respectively and the animals in-group IV were supplemented with 35 ppm Zn as Zn propionate. Semen collection and evaluation was done in the first month (to assess semen quality at the start of the experiment) and 7th, 8th and 9th month of experimental feeding to evaluate the effect of supplemental Zn on semen attributes. We gave 6 months for Zn feeding, so that 3 sperm cycles of spermatogenesis had passed and the collected semen reflected the complete effect of Zn supplementation. Six ejaculates from each bull were collected and evaluated for semen quantitative (ejaculate volume, sperm concentration and sperm number per ejaculate) and qualitative characteristics (semen pH, mass motility, individual motility, sperm livability percent and abnormal sperm percent, percent intact acrosome, bovine cervical mucus penetration test, hypo-osmotic sperm swelling test) and activity of seminal plasma enzymes i.e., alkaline phosphatase, acid phosphatase, GOT and GPT. Testosterone level in the blood serum of crossbred bulls was also estimated. Mean values of semen quantitative and qualitative characteristics at the start of the experiment were statistically non significant (P > 0.05) in all the crossbred cattle bulls, however, there were statistically significant differences among the bulls of different groups after 6 months of zinc supplementation. Mean ejaculate volume (mL) was 2.37, 4.70, 5.86 and 6.38, respectively in groups I to IV, indicating a statistically significant (P < 0.05) higher semen volume in Zn-supplemented groups as compared to the control group of bulls. Similarly, sperm concentration (million.mL −1 ), live sperm (%) and motility (%) were significantly (P < 0.01) higher in Zn-supplemented groups as compared to the control group. The results of BCMPT and HOSST revealed a significant improvement in sperm functional ability in all the groups supplemented with Zn as compared to the control group. The activity of alkaline and acid phosphatase in seminal plasma was significantly (P < 0.05) higher in the Zn-supplemented groups, whereas GOT and GPT activities in seminal plasma were significantly (P < 0.05) lower in the Zn propionate supplemented group as compared to the control group. Testosterone concentration (ng.mL −1 ) in blood serum was significantly higher in animals of groups III and IV, as compared to control group. It may be concluded that Zn supplementation either in the inorganic or organic form in the diet of crossbred bulls improved the qualitative and quantitative attributes of semen; however, the number of sperm per ejaculate, mass motility and semen fertility test like bovine cervical mucus penetration was significantly higher in bulls gi...

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Section: Semen Qualitative Characteristics Of Crossbred Cattle Bulls supporting

confidence: 79%

Effect of different levels and sources of zinc supplementation on quantitative and qualitative semen attributes and serum testosterone level in crossbred cattle (Bos indicus×Bos taurus) bulls

et al. 2006

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“…The present study demonstrated that in male mice, dietary Zn deficiency significantly reduced the level of testosterone and increased the circulating level of LH, thus indicating that the release and synthesis of LH occurred under conditions of Zn depletion. These data are in agreement with previous findings obtained by other researchers, who demonstrated that rats exhibited significant decreases in the serum levels of testosterone and progesterone together with increases in serum levels of follicle-stimulating hormone (FSH) and LH (3,5,44). Furthermore, in vitro experiments have demonstrated that decreased levels of progesterone synthesis by Leydig cells as well as the decreased expression of StAR, P450scc and 3β-HSD in the Leydig cells occurred under conditions of Zn depletion.…”

Section: Discussionsupporting

confidence: 93%

“…It is well known that Zn content is high in the adult testis, and the prostate has a higher concentration of Zn than any other organ of the body. Zn plays pivotal roles in testicular development, the maintenance of testicular function and spermatogenesis (3,4). In humans, for example, Zn is a major constituent of seminal plasma and it is necessary for the formation and maturation of spermatozoa as well as for fertilization (5,6).…”

Section: Introductionmentioning

confidence: 99%

A potential role for zinc transporter 7 in testosterone synthesis in mouse Leydig tumor cells

Chu

Chen

Zhang

et al. 2016

International Journal of Molecular Medicine

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Abstract. Previous studies have demonstrated that zinc (Zn) is an essential trace element which is involved in male reproduction. The zinc transporter (ZnT) family, SLC30a, is involved in the maintenance of Zn homeostasis and in mediating intracellular signaling events; however, relatively little is known regarding the effect of ZnTs on testosterone synthesis. Thus, in the present study, we aimed to determine the effect of Zn transporter 7 (ZnT7) on testosterone synthesis in male CD-1 mice and mouse Leydig cells. The findings of the present study revealed that the concentrations of Zn in the testes and Leydig cells were significantly lower in mice fed a Zn-deficient diet compared with the control mice fed a Zn-adequate diet. In addition, ZnT7 was principally expressed and colocalized with steroidogenic acute regulatory protein (StAR) in the Leydig cells of male CD-1 mice. ZnT7 expression was downregulated in the mice fed a Zn-deficient diet, which led to decreases in the expression of the enzymes involved in testosterone synthesis namely cholesterol side-chain cleavage enzyme (P450scc) and 3β-hydroxysteroid dehydrogenase/D5-D4 isomerase (3β-HSD) as well as decreased serum testosterone levels. These results suggested that Znt7 may be involved in testosterone synthesis in the mouse testes. To examine this hypothesis, we used the mouse Leydig tumor cell line (MLTC-1 cell line) in which the ZnT7 gene had been silenced, in order to gauge the impact of changes in ZnT7 expression on testosterone secretion and the enzymes involved in testosterone synthesis. The results demonstrated that ZnT7 gene silencing downregulated the expression of StAR, P450scc and 3β-HSD as well as progesterone concentrations in the human chorionic gonadotrophin (hCG)-stimulated MLTC-1 cells. Taken together, these findings reveal that ZnT7 may play an important role in the regulation of testosterone synthesis by modulating steroidogenic enzymes, and may represent a therapeutic target in testosterone deficiency.

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“…However, the improvement of the result was not mainly expected. Nonrandomized controlled studies showed that in sub fertile males with idiopathic oligozoospermia; oral zinc supplementation improves sperm concentration (16,17). However, Landau et al (18) presented that using 10 mg supplementation of folic acid daily for one month had no advantage on sperm concentration in 40 normospermic and oligozoospermic males.…”

Section: Discussionmentioning

confidence: 99%

Effects of Zinc Sulfate on Subfertility Related to Male Factors: A Prospective Double-Blind, Randomized, Placebo-Controlled Clinical Trial

Sharifzadeh

Norouzi

Ashrafi

et al. 2016

J Obstet Gynecol Cancer Res

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Zinc, Plasma Androgens and Male Sterility

Cited by 70 publications

References 10 publications

Effect of different levels and sources of zinc supplementation on quantitative and qualitative semen attributes and serum testosterone level in crossbred cattle (Bos indicus×Bos taurus) bulls

Effect of different levels and sources of zinc supplementation on quantitative and qualitative semen attributes and serum testosterone level in crossbred cattle (Bos indicus×Bos taurus) bulls

A potential role for zinc transporter 7 in testosterone synthesis in mouse Leydig tumor cells

Effects of Zinc Sulfate on Subfertility Related to Male Factors: A Prospective Double-Blind, Randomized, Placebo-Controlled Clinical Trial

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