Zinc supplement alleviates redox alterations mediated by doxorubicin-induced testicular oxidative stress

Abstract

Background: This study investigated the activities of zinc (Zn) supplement on redox status of animal model of doxorubicin-induced testicular oxidative stress; in order to ascertain whether Zn supplement could be beneficial on altered testicular redox status mediated by doxorubicin (a known anticancer drug). Methods: Single dose of doxorubicin (10mg/kg b.wt. i.p.) was used to induce testicular oxidative stress on DOX group as well as DOX+Zn group; DOX group was left untreated, while DOX+Zn was treated with Zn (10mg/kg b.wt. p.o.) daily for 14days after induction of testicular oxidative stress. Results were compared with control and Zn-only group as well as between DOX group and DOX+Zn group. Statistical significance was considered at P<0.05. Results: Doxorubicin (DOX) induced testicular oxidative stress by increasing lipid peroxidation (malondialdehyde; MDA) and decreasing glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD) and total antioxidant capacity (TAC) when compared to control, P<0.05. Zn treatment decreased testicular MDA and improved testicular GPx, CAT, SOD and TAC; when Zn-only group is compared to control as well as comparison between DOX group and DOX+Zn group at P<0.05. Conclusion: Zinc supplement alleviates redox alterations mediated by doxorubicin-induced testicular oxidative stress by improving testicular GPx, CAT, SOD, TAC and decreasing testicular MDA; forming basis for inclusion of Zn supplement as a combatant of doxorubicin-induced testicular oxidative stress.

Comparative analysis of the impact of artificially and naturally ripened fruit on obesity-induced biochemical and hematological alterations

“…and used for biochemical and hematological assays. Biochemical parameters such as antioxidants (Malondialdehyde: MDA, Superoxide Dismutase: SOD, Catalase: CAT, Glutathione Peroxidase: GPx) was performed as described by, 13 hepatic enzymes (Aspartate Aminotransferase: AST, Alanine Aminotransferase: ALT, Gamma-Glutamyl Transferase: GGT, Alkaline Phosphatase: ALP) as described by, 14 Kidney function biomarkers (Urea, Creatine, K + , Na + , Cl -) as described by 15 and hematological parameters (Red blood cell: RBC, Packed Cell Volume: PCV, Hemoglobin (Hb) concentration, White blood cell: WBC, WBC differential count) as described by 11 Obesity was also ascertained using Lee index, body weight and size as described by 16 Data obtained was statistically analyzed using GraphPad prism (v8); values were expressed as mean ± standard error of mean (SEM). ANOVA was performed between groups using Boneferroni post hoc comparison test and results were considered significance at P<0.05.…”