Zinc Supplementation Attenuates Cardiac Remodeling After Experimental Myocardial Infarction

Abstract

Background/Aims: The objective of our study was to evaluate the effects of zinc supplementation on cardiac remodeling following acute myocardial infarction in rats. Methods: Animals were subdivided into 4 groups and observed for 3 months: 1) Sham Control; 2) Sham Zinc: Sham animals receiving zinc supplementation; 3) Infarction Control; 4) Infarction Zinc. After the followup period, we studied hypertrophy and ventricular geometry, functional alterations in vivo and in vitro, changes related to collagen, oxidative stress, and inflammation, assessed by echocardiogram, isolated heart study, western blot, flow cytometer, morphometry, and spectrophotometry. Results: Infarction induced a significant worsening of the functional variables. On the other hand, zinc attenuated both systolic and diastolic cardiac dysfunction induced by infarction. Considering the infarct size, there was no difference between the groups. Catalase and superoxide dismutase decreased in infarcted animals, and zinc increased its activity. We found higher expression of collagens I and III in infarcted animals, but there was no effect of zinc supplementation. Likewise, infarcted animals had higher levels of IL-10, but without zinc interference. Nrf-2 values were not different among the groups. Infarction increased the amount of Treg cells in the spleen as well as the amount of total lymphocytes. Zinc increased the amount of CD4+ in infarcted animals, but we did not observe effects in relation to Treg cells. Conclusion: zinc attenuates cardiac remodeling after infarction in rats; this effect is associated with modulation of antioxidant enzymes, but without the involvement of collagens I and III, Nrf-2, IL-10, and Treg cells.

Short-Term Inhalation of Ultrafine Zinc Particles Could Alleviate Cardiac Dysfunctions in Rats of Myocardial Infarction

“…The short-term inhalation of ultrafine zinc particles slowed down cardiac impairments caused by the myocardial infarction. The role of zinc as a modulator of cardiac changes has been recognized, which could affect homeostasis, oxidative stress, immune function, apoptosis, and ageing (Chasapis et al, 2012;Andrea et al, 2018). Zinc, a vital antioxidative element, can protect myocyte membrane against unsaturated lipids and inflammatory cytokines (Hennig et al, 1996) and thus alleviate the infarction-induced heart failure (Shokrzadeh et al, 2009) albeit there was much debate about the role of zinc concentrations in different types of cardiomyopathy (Marin and Rodriguez-Martinez, 1995;Kosar et al, 2006).…”

Association between Reduced Serum Zinc and Diastolic Dysfunction in Maintenance Hemodialysis Patients

“…Moreover, a reduced serum zinc level could be harmful to antioxidant defenses, leading to oxidative stress-induced cardiomyocyte necrosis [ 38 ]. In an animal study, zinc was shown to attenuate cardiac remodeling through the modulation of antioxidant enzymes [ 17 ]. In addition, a randomized control trial evaluating nutritional supplementation, including zinc, in patients with heart failure found an improvement in LV end-diastolic volume after treatment [ 39 ].…”

“…Up to 40–78% of patients undergoing HD have been reported to have a zinc deficiency [ 14 , 15 ]. The effects of pharmacological strategies targeting diastolic dysfunction in HD patients remain unsatisfactory [ 16 ]; however, accumulating evidence indicates that zinc plays a pivotal role in attenuating cardiac remodeling through the modulation of antioxidant enzymes [ 17 , 18 ]. Furthermore, a recent meta-analysis of 27 case–control studies reported significantly lower serum zinc levels in patients with heart failure than in control subjects [ 19 ].…”

Influência do Consumo de Suco de Laranja (Citrus Sinensis) na Remodelação Cardíaca de Ratos Submetidos a Infarto do Miocárdio

“…O tamanho da amostra utilizada baseou-se em outros estudos realizados em nosso laboratório. 3,8,25 O número de ratos no grupo infartado foi maior, uma vez que a mortalidade esperada para esses animais durante o período experimental é de aproximadamente 50%. Além disso, somente animais com área infartada do VE maior que 30% foram incluídos no estudo.…”