Zinc supplementation inhibits the high glucose‑induced EMT of peritoneal mesothelial cells by activating the Nrf2 antioxidant pathway

Gao, Lili; Fan, Yi; Zhang, Xiuli; Yang, Lina; Huang, Wenyu; Hang, Tianyu; Li, Mingyang; Du, Shuyan; Ma, Jun

doi:10.3892/mmr.2019.10260

Cited by 8 publications

(5 citation statements)

References 52 publications

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“…To our knowledge, few data have demonstrated the activation of Nrf2/HO-1 signaling inhibiting ROS production and MMT in HPMCs. However, it was limited to phenomenon observation and lacked mechanism research and efficacy verification [30]. In our study, we illustrated for the first time that asiaticoside can inhibit ROS production by targeting the Nrf2/HO-1 signaling pathway in the PD field.…”

Section: Discussionmentioning

confidence: 95%

Asiaticoside inhibits TGF-β1-induced mesothelial-mesenchymal transition and oxidative stress via the Nrf2/HO-1 signaling pathway in the human peritoneal mesothelial cell line HMrSV5

et al. 2020

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Background: Peritoneal fibrosis (PF) is a frequent complication caused by peritoneal dialysis (PD). Peritoneal mesothelial cells (PMCs), the first barrier of the peritoneum, play an important role in maintaining structure and function in the peritoneum during PD. Mesothelial-mesenchymal transition (MMT) and oxidative stress of PMCs are two key processes of PF. Purpose: To elucidate the efficacy and possible mechanism of asiaticoside inhibition of MMT and ROS generation in TGF-β1-induced PF in human peritoneal mesothelial cells (HPMCs). Methods: MMT and ROS generation of HPMCs were induced by TGF-β1. To explain the anti-MMT and antioxidant role of asiaticoside, varied doses of asiaticoside, oxygen radical scavenger (NAC), TGF-β receptor kinase inhibitor (LY2109761) and Nrf2 inhibitor (ML385) were used separately. Immunoblots were used to detect the expression of signaling associated proteins. DCFH-DA was used to detect the generation of ROS. Transwell migration assay and wound healing assay were used to verify the capacity of asiaticoside to inhibit MMT. Immunofluorescence assay was performed to observe the subcellular translocation of Nrf2 and expression of HO-1. Results: Asiaticoside inhibited TGF-β1-induced MMT and suppressed Smad signaling in a dose-dependent manner. Migration and invasion activities of HPMCs were decreased by asiaticoside. Asiaticoside decreased TGF-β1-induced ROS, especially in a high dose (150 μM) for 6 h. Furthermore, ML385 partly abolished the inhibitory effect of asiaticoside on MMT, ROS and p-Smad2/3. Conclusions: Asiaticoside inhibited the TGF-β1-induced MMT and ROS via Nrf2 activation, thus protecting the peritoneal membrane and preventing PF.

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Section: Discussionmentioning

confidence: 95%

Asiaticoside inhibits TGF-β1-induced mesothelial-mesenchymal transition and oxidative stress via the Nrf2/HO-1 signaling pathway in the human peritoneal mesothelial cell line HMrSV5

et al. 2020

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“…The cells HG + Zn group [HG (126 mM) and ZnSO4 (10 μM)] significantly inhibited the HG‐induced EMT and reversed the HG‐stimulated increase in migration and increase in ROS levels. These data suggest that zinc activates the Nrf2 antioxidant pathway of HG‐induced HPMCs [91].…”

Section: Nutritional Strategies To Mitigate the Inflammationmentioning

confidence: 99%

Using the concept of food as medicine to mitigate inflammation in patients undergoing peritoneal dialysis

Alvarenga,

Reis,

Kemp

et al. 2024

Ther Apher Dial

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The most common kidney replacement therapy (KRT) worldwide is hemodialysis (HD), and only 5%–10% of patients are prescribed peritoneal dialysis (PD) as KRT. Despite PD being a different method, these patients also present particular complications, such as oxidative stress, gut dysbiosis, premature aging, and mitochondrial dysfunction, leading to an inflammation process and high cardiovascular mortality risk. Although recent studies have reported nutritional strategies in patients undergoing HD with attempts to mitigate these complications, more information must be needed for PD patients. Therefore, this review provides a comprehensive analysis of recent studies of nutritional intervention to mitigate inflammation in PD patients.

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“…Gao et al. [ 165 ] showed that Zn supplementation can reduce oxidative stress in peritoneal mesothelial cells (PMcs) to inhibit the effect of high glucose (HG) on epithelial-mesenchymal transition (EMT), thereby, improving peritoneal fibrosis (PF) during peritoneal dialysis. Zn deficiency is a predictor of cardiovascular death in dialysis patients [ 166 ], and decrease of Zn levels in dialysis patients is significantly correlated with diastolic dysfunction [ 167 ].…”

Section: Advances In Research On Trace Elements and Malnutritionmentioning

confidence: 99%

Progress in the study of nutritional status and selenium in dialysis patients

et al. 2023

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Malnutrition is very common in patients with chronic kidney disease, especially in those on maintenance dialysis. Malnutrition is one of the major factors affecting survival and death of dialysis patients, and reducing their activity tolerance and immunity. There are numerous and interacting risk factors for malnutrition, such as reduced nutritional intake, increased energy expenditure, hormonal disorders, and inflammation. Selenium, in the form of selenoproteins, is involved in many physiological processes in the body and plays an important role in maintaining redox homeostasis. Oxidative stress and infection are very common in dialysis patients, and selenium levels in dialysis patients are significantly lower than those in the healthy population. It has been shown that there is a correlation between selenium levels in hemodialysis patients and their nutrition-related indicators, and that selenium supplementation may improve malnutrition in patients. However, further studies are needed to support this conclusion and there is a lack of basic research to further characterize the potential mechanisms by which selenium may improve malnutrition in dialysis patients. The purpose of this review is to provide a comprehensive overview of factors associated with malnutrition in dialysis patients and to describe the progress of research on nutritional status and selenium levels in dialysis patients.

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Zinc supplementation inhibits the high glucose‑induced EMT of peritoneal mesothelial cells by activating the Nrf2 antioxidant pathway

Cited by 8 publications

References 52 publications

Asiaticoside inhibits TGF-β1-induced mesothelial-mesenchymal transition and oxidative stress via the Nrf2/HO-1 signaling pathway in the human peritoneal mesothelial cell line HMrSV5

Asiaticoside inhibits TGF-β1-induced mesothelial-mesenchymal transition and oxidative stress via the Nrf2/HO-1 signaling pathway in the human peritoneal mesothelial cell line HMrSV5

Using the concept of food as medicine to mitigate inflammation in patients undergoing peritoneal dialysis

Progress in the study of nutritional status and selenium in dialysis patients

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