Zingerone ameliorates gentamicin-induced nephrotoxicity in rats

Hosseinzadeh, Azam; Goudarzi, Mehdi; Karimi, Mahdi; Khorsandi, Layasadat; Mehrzadi, Saeed; Mombeini, Mohammad Amin

doi:10.1007/s00580-020-03129-5

Cited by 15 publications

(13 citation statements)

References 37 publications

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“…Despite the shortage of the available data regarding the impact of TG on renal tissue, Dong et al [2] suggested the implication of TG in mitochondrial dysfunction, oxidative damage, and apoptosis in different cancer cell lines. Moreover, there is a strong body of evidence supporting the involvement of oxidative stress and apoptotic cascade in GMinduced renal injury [19,[31][32][33].…”

Section: Discussionmentioning

confidence: 99%

Tigecycline and Gentamicin-Combined Treatment Enhances Renal Damage: Oxidative Stress, Inflammatory Reaction, and Apoptosis Interplay

et al. 2022

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Although the combination of antibiotics is generally well-tolerated, they may have nephrotoxic effects. This study investigated whether tigecycline (TG) and gentamicin (GM) co-administration could accelerate renal damage. Male Wistar rats were randomly divided into six experimental groups: the control, TG7 (tigecycline, 7 mg/kg), TG14 (tigecycline, 14 mg/kg), GM (gentamicin, 80 mg/kg), TG7+GM, and TG14+GM groups. The combination of TG and GM evoked renal damage seen by the disruption of kidney function tests. The perturbation of renal tissue was mainly confounded to the TG and GM-induced oxidative damage, which was exhibited by marked increases in renal MDA (malondialdehyde) along with a drastic reduction in GSH (reduced-glutathione) content and CAT (catalase) activity compared to their individual treatments. More obvious apoptotic events and inflammation were also revealed by elevating the annexin-V and interleukin-6 (IL-6) levels, aside from the upregulation of renal PCNA (proliferating cell nuclear antigen) expression in the TG and GM concurrent treatment. The principal component analysis indicated that creatinine, urea, annexin-V, IL-6, and MDA all played a role in discriminating the TG and GM combined toxicity. Oxidative stress, inflammatory response, and apoptosis were the key mechanisms involved in this potentiated toxicity.

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Section: Discussionmentioning

confidence: 99%

Tigecycline and Gentamicin-Combined Treatment Enhances Renal Damage: Oxidative Stress, Inflammatory Reaction, and Apoptosis Interplay

et al. 2022

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“…This study showed that zingerone with all doses used could decrease the elevated tissue levels of PC in the liver of MTX-treated rats. In another study, Hosseinzadeh et al showed that zingerone could decrease the content of PC in renal tissue in gentamicin-induced nephrotoxicity in rats (41). As protein carbonyl is an indicator of the existence of free radicals (42), it can be concluded that zingerone has an antioxidant property.…”

Section: Discussionmentioning

confidence: 99%

Zingerone Attenuates Methotrexate-Induced Hepatotoxicity in Rats

Goudarzi

Basir

Malayeri

et al. 2021

Jundishapur J Nat Pharm Prod

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Background: Methotrexate (MTX) is mainly used for the chemotherapy of different types of malignancy and some autoimmune diseases like rheumatoid arthritis and inflammatory bowel disease. The MTX application is limited by its severe side effects, including several types of hepatic injury. Objectives: In this study, we decided to evaluate if zingerone (the main constituent of ginger) can reduce the hepatic side effects of MTX. Methods: Thirty-five rats were divided into five groups: Control group receiving normal saline (N/S), once daily, by gavage, for 10 days, and N/S intraperitoneally (i.p.), a single dose on the ninth day; Methotrexate (MTX) group receiving N/S, once daily, by gavage, for 10 days, and MTX (i.p.), a single dose (20 mg/kg) on the ninth day; Groups 3 (ZG25), 4 (ZG50), and 5 (ZG100) receiving zingerone (25, 50, and 100 mg/kg, respectively), once daily, by gavage, for 10 days, and MTX (i.p.), a single dose (20 mg/kg) on the ninth day. Results: The results showed a significant decrease in serum AST, ALT, and ALP, as well as the hepatic content of MDA, NO, PC, TNF-α, and IL-1β, in the ZG groups compared with the MTX group. The activity of SOD, CAT, and GPX, as well as the hepatic content of GSH, showed a significant increase in the ZG groups compared with the MTX group. Histopathological improvement in the hepatic tissue of ZG groups compared with the MTX group confirmed all other findings. Conclusions: It is concluded that zingerone can improve hepatic injury induced by MTX in rats regarding the redox system features, inflammation, and histological changes. This can make humans hopeful for using Ginger in the future for attenuating the hepatic side effects of MTX when used chronically.

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“…The protein oxidation in different groups was determined by measuring the level of protein-bound carbonyl as a biomarker for oxidative stress [ 15 ]. To determine the PC level in testicular tissue , a mixture of the testis tissue homogenate (0.5 mL) and 0.1% DNPH (w/v in 2 N HCl; 0.5 mL) was prepared and incubated at room temperature for 1 h. For precipitation of total protein, ice-cold 20% TCA (v/v; 1 mL) was added to mixture and centrifuged at 10,000 g for 10 min to pellet proteins.…”

Section: Methodsmentioning

confidence: 99%

“…The level of NO• in testicular tissue was determined using the Griess assay [ 15 ]. The supernatant of samples was collected and deproteinized by mixing with zinc sulfate (40 μL, 30% (w/v)).…”

Section: Methodsmentioning

confidence: 99%

The ameliorative effect of ellagic acid on di-(2-ethylhexyl) phthalate-induced testicular structural alterations, oxidative stress, inflammation and sperm damages in adult mice

Hosseinzadeh

Mehrzadi

Siahpoosh

et al. 2021

Reprod Biol Endocrinol

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Background Phthalates such as di (2-ethylhexyl) phthalate (DEHP) are well known exogenous substances, disrupting reproductive system function and structure. The current research demonstrated the effect of ellagic acid (EA) on DEHP-induced testicular injury in mice. Methods Thirty-five healthy adult male mice were randomly divided to five groups; normal saline receiving group, DEHP (2 g/kg/day, dissolved in corn oil, p.o.) receiving group, DEHP (2 g/kg/day, dissolved in corn oil, p.o.) and EA receiving groups (25, 50 and 100 mg/kg/day, p.o.). Treatment duration of animals was 14 days. Body and testes weights and sperm characteristics and histological changes of testes were evaluated. Serum testosterone, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels were analyzed. In the testicular tissue, oxidative/nitrosative stress markers and inflammatory cytokine levels were measured. Results Ellagic acid significantly reduced DEHP-induced reduction of body and testes weights. The DEHP-induced reduction of spermatogonia, primary spermatocyte and sertoli cells numbers as well as reduction of sperm vitality and progressive motility were reversed by EA. Furthermore, EA inhibited DEHP-induced alterations in serum hormone levels. These effects were associated with the reduction of DEHP-induced increased level of oxidative stress and inflammatory responses. Conclusions Ellagic acid considerably inhibits testicular toxicity of DEHP through reducing oxidative/nitrosative stress and inflammatory responses. Our data suggest that EA may be considered as a promising agent to inhibit male reproductive toxicity induced by endocrine disrupting chemicals such as DEHP.

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Zingerone ameliorates gentamicin-induced nephrotoxicity in rats

Cited by 15 publications

References 37 publications

Tigecycline and Gentamicin-Combined Treatment Enhances Renal Damage: Oxidative Stress, Inflammatory Reaction, and Apoptosis Interplay

Tigecycline and Gentamicin-Combined Treatment Enhances Renal Damage: Oxidative Stress, Inflammatory Reaction, and Apoptosis Interplay

Zingerone Attenuates Methotrexate-Induced Hepatotoxicity in Rats

The ameliorative effect of ellagic acid on di-(2-ethylhexyl) phthalate-induced testicular structural alterations, oxidative stress, inflammation and sperm damages in adult mice

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