Zip3 Provides a Link between Recombination Enzymes and Synaptonemal Complex Proteins

Agarwal, Seema; Roeder, G. Shirleen

doi:10.1016/s0092-8674(00)00029-5

Cited by 289 publications

(475 citation statements)

References 39 publications

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“…Notably, the relative number of RAD51 foci associated with ZYP1 decreased over time from 65.4% in leptotene to 36.5% in pachytene (P = 5.6 3 10 210 ) ( Figure 2B). This suggests that RAD51 foci represent nucleoprotein filaments that stay predominantly axis-associated and furthermore that ZYP1 is loaded next to RAD51 at DSB sites, as suggested previously (Agarwal and Roeder, 2000). Decreasing total and ZYP1-associated RAD51 foci numbers during meiotic progression could be explained by depletion of RAD51 at sites of successful meiotic DNA repair (Franklin et al, 1999;Allers and Lichten, 2001;Bugreev et al, 2010).…”

Section: Rad51 and Dmc1 Do Not Colocalize And Form Homotypic Foci Dursupporting

confidence: 68%

The Recombinases DMC1 and RAD51 Are Functionally and Spatially Separated during Meiosis in Arabidopsis

et al. 2012

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Meiosis ensures the reduction of the genome before the formation of generative cells and promotes the exchange of genetic information between homologous chromosomes by recombination. Essential for these events are programmed DNA double strand breaks (DSBs) providing single-stranded DNA overhangs after their processing. These overhangs, together with the RADiation sensitive51 (RAD51) and DMC1 Disrupted Meiotic cDNA1 (DMC1) recombinases, mediate the search for homologous sequences. Current models propose that the two ends flanking a meiotic DSB have different fates during DNA repair, but the molecular details remained elusive. Here we present evidence, obtained in the model plant Arabidopsis thaliana, that the two recombinases, RAD51 and DMC1, localize to opposite sides of a meiotic DSB. We further demonstrate that the ATR kinase is involved in regulating DMC1 deposition at meiotic DSB sites, and that its elimination allows DMC1-mediated meiotic DSB repair even in the absence of RAD51. DMC1's ability to promote interhomolog DSB repair is not a property of the protein itself but the consequence of an ASYNAPTIC1 (Hop1)-mediated impediment for intersister repair. Taken together, these results demonstrate that DMC1 functions independently and spatially separated from RAD51 during meiosis and that ATR is an integral part of the regular meiotic program.

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Section: Rad51 and Dmc1 Do Not Colocalize And Form Homotypic Foci Dursupporting

confidence: 68%

The Recombinases DMC1 and RAD51 Are Functionally and Spatially Separated during Meiosis in Arabidopsis

et al. 2012

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“…2A; Peoples et al 2002). Msh4 has been reported to colocalize with Msh5 (Agarwal and Roeder 2000). Accordingly, we found that an msh4⌬ single mutant also exhibited the same level of allelic interactions as msh5⌬ and wild type ( Fig.…”

Section: Cre/loxp Recombination Indicates Allelic Interactionssupporting

confidence: 62%

Multiple branches of the meiotic recombination pathway contribute independently to homolog pairing and stable juxtaposition during meiosis in budding yeast

Peoples-Holst

Burgess

2005

Genes Dev.

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A unique aspect of meiosis is the segregation of homologous chromosomes at the meiosis I division. Homologs are physically connected prior to segregation by crossing over between nonsister chromatids. Crossovers arise from the repair of induced double-strand breaks (DSBs). In many organisms, more DSBs are formed than crossovers in a given nucleus. It has been previously suggested that repair of DSBs to noncrossover recombination products aids homolog alignment. Here we explore how two modes of the meiotic recombination pathway (crossover and noncrossover) and meiotic telomere reorganization contribute to the pairing and close juxtaposition of homologous chromosomes in budding yeast. We found that intermediates in the DSB repair pathway leading to both crossover and noncrossover recombination products contribute independently to close, stable homolog juxtaposition (CSHJ), a measurable state of homolog pairing. Analysis of the ndj1⌬ mutant indicates that the effect of meiotic telomere reorganization on CSHJ is exerted through recombination intermediates at interstitial chromosomal loci, perhaps through the noncrossover branch of the DSB repair pathway. We suggest that transient, early DSB-initiated interactions, including those that give rise to noncrossovers, are important for homolog recognition and juxtaposition.[Keywords: Homolog; meiosis; pairing; recombination; telomeres]Received December 28, 2004; revised version accepted February 15, 2005. Meiosis is the process in which a parent diploid cell undergoes two rounds of chromosome segregation, after one round of replication, to yield four haploid gametes. A universal component of meiosis I is the reductional segregation of homologous chromosomes. Nondisjunction, or improper segregation of homologs, at this stage can lead to gamete aneuploidy. Three factors contribute to the tension required for the reductional segregation of homologs at anaphase I in most organisms: (1) crossing over between homologous chromosomes, (2) cohesion between sister chromatids, and (3) monopolar spindle attachment of sister chromatids at metaphase (Page and Hawley 2003).Crossing over involves the reciprocal exchange of chromosome arms and can be visualized at late stages of meiotic prophase as chiasmata. Such exchange events are a manifestation of double-strand break (DSB)-promoted homologous recombination. Formation and repair of meiotically induced DSBs involve both DSB repair enzymes and meiosis-specific factors (for reviews, see Zickler and Kleckner 1999;Keeney 2001). Meiosis-specific factors bias recombination between homologous chromosomes instead of sister chromatids (Schwacha and Kleckner 1997) and ensure that at least one crossover occurs between every homolog pair (for review, see Bishop and Zickler 2004).A key event in crossover formation is the recognition and pairing of homologous chromosomes. An outstanding question is what brings homologs together in meiosis. Is it early molecular events, late-forming molecules in which homologs are clearly linked by chemical or hyd...

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“…PCR was used to introduce a NotI site $290 bp upstream of the OSW1 ORF and an NheI site just before the stop codon. The NotI-NheI fragment ($1.1 kbp) containing OSW1 was subcloned into the NotI-NheI sites of pSA111 (Agarwal and Roeder 2000) to create pJ83, which contains GFP fused to the 39 end of OSW1.…”

Section: Methodsmentioning

confidence: 99%

SSP2 and OSW1, Two Sporulation-Specific Genes Involved in Spore Morphogenesis in Saccharomyces cerevisiae

Agarwal

Roeder

2007

Genetics

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Spore formation in Saccharomyces cerevisiae requires the synthesis of prospore membranes (PSMs) followed by the assembly of spore walls (SWs). We have characterized extensively the phenotypes of mutants in the sporulation-specific genes, SSP2 and OSW1, which are required for spore formation. A striking feature of the osw1 phenotype is asynchrony of spore development, with some spores displaying defects in PSM formation and others spores in the same ascus blocked at various stages in SW development. The Osw1 protein localizes to spindle pole bodies (SPBs) during meiotic nuclear division and subsequently to PSMs/SWs. We propose that Osw1 performs a regulatory function required to coordinate the different stages of spore morphogenesis. In the ssp2 mutant, nuclei are surrounded by PSMs and SWs; however, PSMs and SWs often also encapsulate anucleate bodies both inside and outside of spores. In addition, the SW is not as thick as in wild type. The ssp2 mutant defect is partially suppressed by overproduction of either Spo14 or Sso1, both of which promote the fusion of vesicles at the outer plaque of the SPB early in PSM formation. We propose that Ssp2 plays a role in vesicle fusion during PSM formation.S PORULATION in the budding yeast, Saccharomyces cerevisiae, involves two overlapping processesmeiosis and spore morphogenesis. During meiosis, a diploid cell undergoes a single round of DNA replication, followed by two rounds of chromosome segregation, to generate four haploid nuclei. During spore morphogenesis, the four daughter nuclei are packaged into spores, which are contained within an ascus.Spore morphogenesis (reviewed by Neiman 2005) initiates with enlargement of the outer surface of the spindle pole body (SPB) to form the meiotic outer plaque. As cells undergo meiosis II, vesicles coalesce on the meiotic plaque to form a novel intracellular membrane called the prospore membrane (PSM). The doublelayered PSM extends outward from each SPB to surround the adjacent nucleus. When the leading edges of the PSM meet and become fused, the haploid nucleus is fully encapsulated to form a prospore. PSM closure triggers the final step of spore morphogenesis, spore wall (SW) formation. The SW is assembled between the two membranes of the PSM and consists of four layers. The two inner layers are composed of glucan and mannan, which are components of vegetative cell walls.

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Zip3 Provides a Link between Recombination Enzymes and Synaptonemal Complex Proteins

Cited by 289 publications

References 39 publications

The Recombinases DMC1 and RAD51 Are Functionally and Spatially Separated during Meiosis in Arabidopsis

The Recombinases DMC1 and RAD51 Are Functionally and Spatially Separated during Meiosis in Arabidopsis

Multiple branches of the meiotic recombination pathway contribute independently to homolog pairing and stable juxtaposition during meiosis in budding yeast

SSP2 and OSW1, Two Sporulation-Specific Genes Involved in Spore Morphogenesis in Saccharomyces cerevisiae

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