Zn (II)-porphyrin-based photochemically green synthesis of novel ZnTPP/Cu nanocomposites with antibacterial activities and cytotoxic features against breast cancer cells
Abstract:This study focuses on synthesizing novel nanocomposites, zinc(II)tetrakis(4-phenyl)porphyrin/Cu nanoparticles (ZnTPP/Cu-NPs),with antibacterial activity, fabricated through a single-step green procedure. In this regard, the self-assembly of ZnTPP was carried out through an acid–base neutralization method to prepare ZnTPP nanoparticles. Then, the copper nanoparticles (Cu-NPs) were grown on ZnTPP nanoparticles through a visible-light irradiated photochemical procedure in the absence and presence of polyacrylic a… Show more
“…The nonappearance of these two signals in the spectra of ZnTPP indicates the zinc coordination in the complex. Moreover, regarding the spectrum of H 2 TPP, the signal at 1606 cm −1 , which is attributed to the C=N stretching vibration of pyrrole, splits into two peaks at around 1595 and 1652 cm −1 indicating the presence of the Zn–N coordination band in the spectra of the prepared ZnTPP NPs which have shifted to 1630 and 1750 nanocomposites formation 24 , 40 – 42 .…”
Section: Resultsmentioning
confidence: 97%
“…2 Ad). Figure 2 ( A ) PXRD of a simulated pattern of ZnTPP NPs (a), ZnTPP NPs (b) 42 , ZnTPP/Ag NCs (c), ZnTPP/Ag/AgCl/Cu NCs (d), ZnTPP/Au/Ag/AgCl NCs (e), ( B ): UV–Vis spectra of H 2 TPP (a), ZnTPP (b), ZnTPP NPs (c) 42 , ZnTPP/Ag/AgCl NCs (d), ZnTPP/Ag NCs (e), ZnTPP/Au/Ag/AgCl NCs (f), ( C ): FT-IR spectra of H 2 TPP (a), ZnTPP (b), ZnTPP NPs (c) 42 , ZnTPP/Ag NCs (d), ZnTPP/Ag/AgCl/Cu NCs (e), ZnTPP/Au/Ag/AgCl NCs (f). …”
In this research, the one-step synthesis of novel porphyrin-based nanocomposites was performed easily using a photochemical under visible light illumination strategy. As a result, the focus of this research is on synthesizing and using decorated ZnTPP (zinc(II)tetrakis(4-phenyl)porphyrin) nanoparticles with Ag, Ag/AgCl/Cu, and Au/Ag/AgCl nanostructures as antibacterial agents. Initially, ZnTPP NPs were synthesized as a result of the self-assembly of ZnTPP. In the next step, in a visible-light irradiation photochemically process, the self-assembled ZnTPP nanoparticles were used to make ZnTPP/Ag NCs, ZnTPP/Ag/AgCl/Cu NCs, and ZnTPP/Au/Ag/AgCl NCs. A study on the antibacterial activity of nanocomposites was carried out for Escherichia coli, and Staphylococcus aureus as pathogen microorganisms by the plate count method, well diffusion tests, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) values determination. Thereafter, the reactive oxygen species (ROS) were determined by the flow cytometry method. All the antibacterial tests and the flow cytometry ROS measurements were carried out under LED light and in dark. The (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was applied to investigate the cytotoxicity of the ZnTPP/Ag/AgCl/Cu NCs, against Human foreskin fibroblast (HFF-1) normal cells. Due to the specific properties such as admissible photosensitizing properties of porphyrin, mild reaction conditions, high antibacterial properties in the presence of LED light, crystal structure, and green synthesis, these nanocomposites were recognized as kinds of antibacterial materials that are activated in visible light, got the potential for use in a broad range of medical applications, photodynamic therapy, and water treatment.
“…The nonappearance of these two signals in the spectra of ZnTPP indicates the zinc coordination in the complex. Moreover, regarding the spectrum of H 2 TPP, the signal at 1606 cm −1 , which is attributed to the C=N stretching vibration of pyrrole, splits into two peaks at around 1595 and 1652 cm −1 indicating the presence of the Zn–N coordination band in the spectra of the prepared ZnTPP NPs which have shifted to 1630 and 1750 nanocomposites formation 24 , 40 – 42 .…”
Section: Resultsmentioning
confidence: 97%
“…2 Ad). Figure 2 ( A ) PXRD of a simulated pattern of ZnTPP NPs (a), ZnTPP NPs (b) 42 , ZnTPP/Ag NCs (c), ZnTPP/Ag/AgCl/Cu NCs (d), ZnTPP/Au/Ag/AgCl NCs (e), ( B ): UV–Vis spectra of H 2 TPP (a), ZnTPP (b), ZnTPP NPs (c) 42 , ZnTPP/Ag/AgCl NCs (d), ZnTPP/Ag NCs (e), ZnTPP/Au/Ag/AgCl NCs (f), ( C ): FT-IR spectra of H 2 TPP (a), ZnTPP (b), ZnTPP NPs (c) 42 , ZnTPP/Ag NCs (d), ZnTPP/Ag/AgCl/Cu NCs (e), ZnTPP/Au/Ag/AgCl NCs (f). …”
In this research, the one-step synthesis of novel porphyrin-based nanocomposites was performed easily using a photochemical under visible light illumination strategy. As a result, the focus of this research is on synthesizing and using decorated ZnTPP (zinc(II)tetrakis(4-phenyl)porphyrin) nanoparticles with Ag, Ag/AgCl/Cu, and Au/Ag/AgCl nanostructures as antibacterial agents. Initially, ZnTPP NPs were synthesized as a result of the self-assembly of ZnTPP. In the next step, in a visible-light irradiation photochemically process, the self-assembled ZnTPP nanoparticles were used to make ZnTPP/Ag NCs, ZnTPP/Ag/AgCl/Cu NCs, and ZnTPP/Au/Ag/AgCl NCs. A study on the antibacterial activity of nanocomposites was carried out for Escherichia coli, and Staphylococcus aureus as pathogen microorganisms by the plate count method, well diffusion tests, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) values determination. Thereafter, the reactive oxygen species (ROS) were determined by the flow cytometry method. All the antibacterial tests and the flow cytometry ROS measurements were carried out under LED light and in dark. The (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was applied to investigate the cytotoxicity of the ZnTPP/Ag/AgCl/Cu NCs, against Human foreskin fibroblast (HFF-1) normal cells. Due to the specific properties such as admissible photosensitizing properties of porphyrin, mild reaction conditions, high antibacterial properties in the presence of LED light, crystal structure, and green synthesis, these nanocomposites were recognized as kinds of antibacterial materials that are activated in visible light, got the potential for use in a broad range of medical applications, photodynamic therapy, and water treatment.
“…In our previous research, the cytotoxic effect of porphyrin-based copper nanocomposites was confirmed on breast cancer cells . Herein, considering the biocompatibility, photoactivity, and applicable cancer therapy of a porphyrinic MOF [composed of a Zr 6 O 4 (OH) 4 cluster and a porphyrin ligand (H 2 TCPP)], in a green photochemical strategy, PCN-224/Au-NPs were produced.…”
Section: Introductionmentioning
confidence: 99%
“…41,42 In our previous research, the cytotoxic effect of porphyrinbased copper nanocomposites was confirmed on breast cancer cells. 43 Herein, considering the biocompatibility, photoactivity, and applicable cancer therapy of a porphyrinic MOF [composed of a Zr 6 O 4 (OH) 4 cluster and a porphyrin ligand (H 2 TCPP)], in a green photochemical strategy, PCN-224/Au-NPs were produced. The investigation of the cytotoxic effect of the synthesized porpyrinic MOF-based nanocomposite was carried out utilizing the 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay on colorectal cancer cells and human embryonic kidney (HEK)-293 normal cells as well.…”
In
this work, a simple green synthesis method of the novel metal–organic
framework (MOF) nanocomposite PCN-224/Au-NPs (Au-NPs = gold nanoparticles)
is described. In this regard, initially, PCN-224 was synthesized.
Afterward, in a single-step, one-pot procedure, under visible-light
irradiation, Au-NPs were fabricated on PCN-224. The cytotoxicity effect
of the synthesized PCN-224/Au-NPs nanocomposite was investigated in
human colon cancer cells. Determination of the apoptosis induction
was done by the Annexin- V/propidium iodide flow cytometry method.
Besides, to ascertain the biocompatibility of the synthesized sample,
the cytotoxicity of PCN-224/Au-NPs was evaluated on the human embryonic
kidney (HEK)-293 cell line. The substantial anticancer activity with
the biocompatibility of the structure, the green facile synthesis,
and the MOF surface of the synthesized nanocomposite make it special
for utilization in therapeutic applications.
“…As a result, finding new photocatalyst materials has always been necessary to improve degradation. These materials have a variety of applications, 7 including reduction of CO 2 , degradation of organic contaminants, removal of toxic ions and heavy metal ions, water or air purification, self‐cleaning, and antibacterial properties 8,9 . ZnO has many features to become an environmentally friendly metal oxide and properties, such as inexpensive, safe, low toxicity, biodegradable, and easily prepared 10 .…”
This work effectively synthesized pure ZnO (PZ) and the co‐doped ZnO as Sm‐La CDZ NPs, La‐Sr CDZ NPs, and Sm‐Sr CDZ NPs using a hydrothermal technique. To characterize synthetic nanomaterials used, several techniques, including X‐ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Raman spectra, ultraviolet and visible (UV/Vis), photoluminescence (PL), scanning electron microscopy (SEM), and graphs by energy dispersive X‐ray spectroscopy (XPS), Zeta potential, point of (pHpzc), and specific surface areas. Furthermore, the XRD, SEM, and TEM confirmed the hexagonal crystalline structure. However, XPS and EDX showed that Sm3+, La3+, and Sr2+ ions integrated into the ZnO lattice. The UV‐estimated band gaps increased in the co‐doped ZnO; BET surface areas declined. The Zeta potential proved the positivity surface charge of nanomaterials. Consequently, they utilized it to investigate the breakdown of reactive red 43 (RR43). The degradation percentages for PZ, Sm‐La CDZ NPs, La‐Sr CDZ NPs, and Sm‐Sr CDZ NPs were 72.88%, 82.63%, 87.08%, and 91.31%, respectively. According to the results, the Sm‐Sr CDZ NPs exhibit high photocatalytic activity. In addition, the pseudo‐first‐order kinetic model and Langmuir model were a better fit. The photocatalytic nanomaterials were also recyclable, which added to their stability. The prepared nanoparticles were evaluated against four bacterial strains and two fungal pathogenic, and the result exhibited a broad spectrum against tested strains. The co‐doped NPs revealed MIC values ranging between1.95 and 62.5 μg/mL and MBC values of (31.3–250 μg/mL) compared with PZ (MIC = 7.81–62.5 μg/mL and MBC = 31.3–250 μg/mL) against bacterial strains. Surprisingly, most of these NPs expressed bacteriocidal and fungicidal potential. In silico molecular docking simulation suggested that the antibacterial activity may be related to the inhibition of DNA gyrase, cell wall synthesis (Upps and Fos A), and biofilm activity (PqsR).
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