Zn(II) suppresses biofilm formation in <i>Bacillus amyloliquefaciens</i> by inactivation of the Mn(II) uptake

Huang, Ziyang; Wu, Liming; Li, Xi; Ma, Liumin; Borriss, Rainer; Gao, Xuewen

doi:10.1111/1462-2920.14859

Cited by 9 publications

(7 citation statements)

References 51 publications

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“…Combined with the reduction in epsH expression, it seems that zinc's anti-biofilm mode of action involves interfering with the construction of an extracellular matrix. A recent study demonstrated the effect of Zn 2+ ions on biofilm formation in B. amyloliquefaciens, a root-colonizing bacterium [42]. Similar to our results, the presence of zinc inhibited biofilm formation by interfering with the synthesis of the same extracellular matrix components.…”

Section: Discussionsupporting

confidence: 91%

Mitigating Milk-Associated Bacteria through Inducing Zinc Ions Antibiofilm Activity

Hutchings

Rajasekharan

Reifen

et al. 2020

Foods

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Dairy products are a sector heavily impacted by food loss, often due to bacterial contaminations. A major source of contamination is associated with the formation of biofilms by bacterial species adopted to proliferate in milk production environment and onto the surfaces of milk processing equipment. Bacterial cells within the biofilm are characterized by increased resistance to unfavorable environmental conditions and antimicrobial agents. Members of the Bacillus genus are the most commonly found spoilage microorganisms in the dairy environment. It appears that physiological behavior of these species is somehow depended on the availability of bivalent cations in the environment. One of the important cations that may affect the bacterial physiology as well as survivability are Zn2+ ions. Thus, the aim of this study was to examine the antimicrobial effect of Zn2+ ions, intending to elucidate the potential of a zinc-based antibacterial treatment suitable for the dairy industry. The antimicrobial effect of different doses of ZnCl2 was assessed microscopically. In addition, expression of biofilm related genes was evaluated using RT-PCR. Analysis of survival rates following heat treatment was conducted in order to exemplify a possible applicative use of Zn2+ ions. Addition of zinc efficiently inhibited biofilm formation by B. subtilis and further disrupted the biofilm bundles. Expression of matrix related genes was found to be notably downregulated. Microscopic evaluation showed that cell elongation was withheld when cells were grown in the presence of zinc. Finally, B. cereus and B. subtilis cells were more susceptible to heat treatment after being exposed to Zn2+ ions. It is believed that an anti-biofilm activity, expressed in downregulation of genes involved in construction of the extracellular matrix, would account for the higher sensitivity of bacteria during heat pasteurization. Consequently, we suggest that Zn2+ ions can be of used as an effective antimicrobial treatment in various applications in the dairy industry, targeting both biofilms and vegetative bacterial cells.

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Section: Discussionsupporting

confidence: 91%

Mitigating Milk-Associated Bacteria through Inducing Zinc Ions Antibiofilm Activity

Hutchings

Rajasekharan

Reifen

et al. 2020

Foods

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“…We specifically focused on four B. amyloliquefaciens traits that have previously been linked to plant growth promotion: swarming motility, biomass production, biofilm formation, and direct pathogen suppression via antibiosis ( Chen et al, 2013 ; Huang et al, 2020 ; Fira et al, 2018 ; Moreno-Velandia et al, 2019 ). While most transposon insertions reduced the strains’ performance, some of them improved at least one measured trait.…”

Section: Discussionmentioning

confidence: 99%

Engineering multifunctional rhizosphere probiotics using consortia of Bacillus amyloliquefaciens transposon insertion mutants

Li,

Yang,

Jousset

et al. 2023

eLife

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While bacterial diversity is beneficial for the functioning of rhizosphere microbiomes, multi-species bioinoculants often fail to promote plant growth. One potential reason for this is that competition between different species of inoculated consortia members creates conflicts for their survival and functioning. To circumvent this, we used transposon insertion mutagenesis to increase the functional diversity within Bacillus amyloliquefaciens bacterial species and tested if we could improve plant growth-promotion by assembling consortia of highly clonal but phenotypically dissimilar mutants. While most insertion mutations were harmful, some significantly improved B. amyloliquefaciens plant growth-promotion traits relative to the wild-type strain. Eight phenotypically distinct mutants were selected to test if their functioning could be improved by applying them as multifunctional consortia. We found that B. amyloliquefaciens consortium richness correlated positively with plant root colonization and protection from Ralstonia solanacearum phytopathogenic bacterium. Crucially, 8-mutant consortium consisting of phenotypically dissimilar mutants performed better than randomly assembled 8-mutant consortia, suggesting that improvements were likely driven by consortia multifunctionality instead of consortia richness. Together, our results suggest that increasing intra-species phenotypic diversity could be an effective way to improve probiotic consortium functioning and plant growth-promotion in agricultural systems.

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“…We specifically focused on four B. amyloliquefaciens traits that have previously been linked to plant growth-promotion: swarming motility, biomass production, biofilm formation and direct pathogen suppression via antibiosis (69)(70)(71)(72). While most insertions reduced the strains' performance overall, several of them were associated with improvements regarding at least one measured trait.…”

Section: Discussionmentioning

confidence: 99%

“…Increased biofilm formation was also linked to insertion in hut system ( hutU ) with one mutant, while the other mutant had insertion in YsnB gene, which encodes for a putative metallophosphoesterase. While both insertions were linked to trade-offs with swarming motility and biomass production, they are not commonly associated with biofilm formation in Bacillus (18, 70). Finally, moderate improvement in pathogen suppression was observed with two mutants that had insertions in nhaC (sodium-proton antiporter) and dfnG (difficidin polyketide synthesis) genes.…”

Section: Discussionmentioning

confidence: 99%

“…The wild-type strain and 479 randomly selected mutants (out of a total of 2000 created mutants) were phenotyped for following plant-growth promoting traits: swarming motility, biomass production, biofilm formation and pathogen suppression via production of antibiotics. These traits were selected due to their known importance for B. amyloliquefaciens competitiveness in the rhizosphere and their involvement in pathogen suppression (69)(70)(71)(72). To prepare bacterial inoculants, frozen colonies were picked and pre-grown overnight in LB at 37 °C, washed three times in 0.85% NaCl and adjusted to density of 10 8 cells mL -1 .…”

Section: Phenotypic Characterization Of B Amyloliquefaciens T-5 Mutan...mentioning

confidence: 99%

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Engineering ecologically complementary rhizosphere probiotics using consortia of specialized bacterial mutants

Yang

Jousset

et al. 2022

Preprint

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While bacterial diversity is beneficial for the functioning of rhizosphere microbiomes, multi-species bioinoculants often fail to promote plant growth. One potential reason for this is that competition between inoculated consortia members create conflicts for their survival and functioning. To circumvent this, we used transposon mutagenesis to increase the functional diversity within Bacillus amyloliquefaciens bacterial species and tested if we could improve plant growth-promotion by assembling consortia of closely related but functionally specialized mutants. While most insertion mutations were harmful, some improved strains' plant growth-promotion traits without increasing antagonism between them. Crucially, plant growth-promotion could be improved by applying these specialist mutants as consortia, leading to clear positive relationships between consortia richness, plant root colonization and protection from bacterial wilt disease. Together, our results suggest that increasing intra-species diversity could be an effective way to increase probiotic consortia multifunctionality, leading to more stable plant growth-promotion throughout growth cycle via insurance effects.

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Zn(II) suppresses biofilm formation in Bacillus amyloliquefaciens by inactivation of the Mn(II) uptake

Cited by 9 publications

References 51 publications

Mitigating Milk-Associated Bacteria through Inducing Zinc Ions Antibiofilm Activity

Mitigating Milk-Associated Bacteria through Inducing Zinc Ions Antibiofilm Activity

Engineering multifunctional rhizosphere probiotics using consortia of Bacillus amyloliquefaciens transposon insertion mutants

Engineering ecologically complementary rhizosphere probiotics using consortia of specialized bacterial mutants

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