MnCo2O4 nanorods (MCO) are successfully fabricated by microwave-assisted hydrothermal method. MCO is coated on the commercial screen-printed electrode (SPE), aiming for effective detection of copper (II) ions (Cu2+). The morphological and structural characteristics are investigated through x-ray diffraction (XRD), Raman spectra, and scanning electron microscope (SEM) analysis. Besides, electrochemical properties and performance to detect Cu2+ on the modified electrode (MCO/SPE) are studied in detail using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and differential pulse anodic stripping voltammetry (DPASV) measurements. The results show the MCO/SPE display magnificent enhancements in dynamic linear range, sensitivity, limit of detection (LOD), selectivity, and stability. Under optimized conditions, the prepared electrochemical sensor had a linear range of 0.001–100 μM, corresponding to LOD of 0.72 nM. Notably, the achieved results point out the great potential of the developed sensor based on MnCo2O4 nanorods for heavy metal ion detection.