ZnO, Ag and ZnO-Ag nanoparticles exhibit differential modes of toxic and oxidative action in hemocytes of mussel Mytilus galloprovincialis

Efthimiou, Ioanna; Kalamaras, Georgios; Papavasileiou, Katerina; Anastasi-Papathanasi, Natalia; Georgiou, Yiannis; Dailianis, Stefanos; Deligiannakis, Yiannis; Vlastos, Dimitris

doi:10.1016/j.scitotenv.2020.144699

Cited by 18 publications

(9 citation statements)

References 83 publications

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“…In this work, ZnO-NP pretreatment saved larvae from C. albicans infection at a dose of 2 mg/kg without apparent toxicity, which is manifested by reducing the larval mortality and the fungal burden in hemolymph and tissue. The mechanisms driving antimicrobial activity of ZnO-NPs have been described in the literature, including generation of ROS, the release of zinc ions, and interaction of ZnO-NPs with the surfaces of microorganisms (Behzad et al, 2021;Efthimiou et al, 2021). Particularly, these mechanisms mainly focus on the directly antimicrobial properties of NPs.…”

Section: Discussionmentioning

confidence: 99%

“…However, the application of Ag-NPs has drawn public concerns due to the side effects, including toxicity, oxidative stress, and inflammatory response (Burns et al, 2021). Different from Ag-NPs, zinc oxide nanoparticles (ZnO-NPs) are safer to mammalian cells (Bondarenko et al, 2013;Efthimiou et al, 2021). Bondarenko et al (2013) pointed out that the median lethal doses (LD50) of Ag-NPs and ZnO-NPs for mammalian cells were 11.3 and 43 mg/L, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Zinc Oxide Nanoparticles Prime a Protective Immune Response in Galleria mellonella to Defend Against Candida albicans

Pan

et al. 2021

Front. Microbiol.

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Purpose: Zinc oxide nanoparticles (ZnO-NPs) have exerted antimicrobial properties. However, there is insufficient evaluation regarding the in vivo antifungal activity of ZnO-NPs. This study aimed to investigate the efficacy and mechanism of ZnO-NPs in controlling Candida albicans in the invertebrate Galleria mellonella.Methods:Galleria mellonella larvae were injected with different doses of ZnO-NPs to determine their in vivo toxicity. Non-toxic doses of ZnO-NPs were chosen for prophylactic injection in G. mellonella followed by C. albicans infection. Then the direct in vitro antifungal effect of ZnO-NPs against C. albicans was evaluated. In addition, the mode of action of ZnO-NPs was assessed in larvae through different assays: quantification of hemocyte density, morphology observation of hemocytes, characterization of hemocyte aggregation and phagocytosis, and measurement of hemolymph phenoloxidase (PO) activity.Results: Zinc oxide nanoparticles were non-toxic to the larvae at relatively low concentrations (≤20 mg/kg). ZnO-NP pretreatment significantly prolonged the survival of C. albicans-infected larvae and decreased the fungal dissemination and burden in the C. albicans-infected larvae. This observation was more related to the activation of host defense rather than their fungicidal capacities. Specifically, ZnO-NP treatment increased hemocyte density, promoted hemocyte aggregation, enhanced hemocyte phagocytosis, and activated PO activity in larvae.Conclusion: Prophylactic treatment with lower concentrations of ZnO-NPs protects G. mellonella from C. albicans infection. The innate immune response primed by ZnO-NPs may be part of the reason for the protective effects. This study provides new evidence of the capacity of ZnO-NPs in enhancing host immunity and predicts that ZnO-NPs will be attractive for further anti-infection applications.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Zinc Oxide Nanoparticles Prime a Protective Immune Response in Galleria mellonella to Defend Against Candida albicans

Pan

et al. 2021

Front. Microbiol.

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“…While catalase was induced by either nTiO 2 or Au-coated nTiO 2 , oxidative damage (LPO) was more strongly expressed with uncoated nTiO 2 . Dissolved but not nAu-inhibited AChE in the hemolymph of Mytilus galloprovinciallis suggests that ionic forms of the metal acted on the enzyme [33]. Inhibition of AChE in fish exposed to nAg was observed in muscles and brains [34].…”

Section: Discussionmentioning

confidence: 99%

“…The capacity of nanoparticles to absorb existing chemicals, such as PAHs and other chemicals, e.g., pharmaceuticals or pesticides, is another property of nanomaterials referred to collectively as "Trojan horse effects" [36,37]. Surface properties could also be changed by surface interactions, as shown above, involving the adsorbtion of proteins, lipids or other macromolecules (natural organic matter) at the surface of nanoparticles [33]. The formation of protein or lipid coronae at the surface of nAg was shown to preserve the Ag core, thereby limiting rapid diffusion of ionic Ag + into the medium and toxicity [38].…”

Section: Discussionmentioning

confidence: 99%

The Influence of Silver Nanoparticle Form on the Toxicity in Freshwater Mussels

et al. 2022

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The contribution of the form of silver nanomaterials (nAg) towards toxicity in aquatic organisms is not well understood. The purpose of this study was to examine the toxicity of various structures (sphere, cube and prism) of nAg in Dreissena bugensis mussels. Mussels were exposed to increasing concentrations of polyvinyl-coated nAg of the same size for 96 h at 15 °C. They were then analyzed for biophysical changes in the cytoplasm (viscosity, protein aggregation and lipids), neuro-activity (fractal kinetics of acetylcholinesterase (AChE)), oxidative stress (labile zinc (Zn) and lipid peroxidation) and inflammation (arachidonate cyclooxygenase). Although some decreasing effects in protein aggregation were observed, viscosity was more strongly decreased in mussels exposed to spheric and prismatic nAg. The activity of AChE was significantly decreased in the following form-dependent manner: prismatic > cubic > spheric nAg. The fractal dimension of AChE reactions was reduced by all geometries of nAg, while dissolved Ag had no effects. For nanoparticles with the same coating and relative size, spheric nAg produced more significant changes towards the fractal dimension of AChE, while prismatic nAg increased both protein aggregation and viscosity, whereas cubic nAg decreased protein aggregation in the cytoplasm. It is concluded that the geometries of nanoparticles could influence toxicity in aquatic organisms.

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“…The induction of inflammation effect in the gills of mussels exposed to nAg related directly to nanoparticle size and exposure time (Bouallegui et al, 2017). More recently custom made, nZnO promote lipid peroxidation and immune-related generation of ROS, like superoxide anion ( • O 2 − ) and nitric oxide (NO) in hemocytes of M. galloprovincialis presumably as a result of protein biomolecular corona modulating the NPs surface energy and uptake efficiency (Efthimiou et al, 2021). Formation of endogenous corona on the 150 nm nPS-NH 2 dominated by 180 kDa Toposome precursor was shown in the coelomic fluid of purple sea urchin P. lividus (Marques-Santos et al, 2018).…”

Section: Endogenous Biomolecular Corona Formation In Invertebratesmentioning

confidence: 99%

Aquatic organisms modulate the bioreactivity of engineered nanoparticles: focus on biomolecular corona

Liu

Worms²,

Jakšić³

2022

Front. Toxicol.

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The increased use of nanoparticle (NP)-enabled materials in everyday-life products have raised concerns about their environmental implications and safety. This motivated the extensive research in nanoecotoxicology showing the possibility that NPs could cause harm to the aquatic organisms if present at high concentrations. By contrast, studies dealing with influence that organisms could exert on the fate and thus effects of NPs are still very rare. Drawing on the existing up-to-date knowledge we critically discuss the formation of biomolecular corona as one of the mechanisms by which organisms exerted control on the NPs fate in the aquatic and biotic environments. We focused the formation of corona by exogeneous and endogenous biomolecules and illustrated the discussion with the specific example of phytoplankton and aquatic invertebrate species. We highlighted the necessity to incorporate the concept of biomolecular corona within more general framework considering the feedback of aquatic organisms and the control they exert in shaping the fate and impact of NPs in the aquatic and biological environment. In our view such broader perspective will contribute to get novel insights into the drivers of environmental transformations of NPs and their mechanisms, which are important in environmental risk assessment.

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ZnO, Ag and ZnO-Ag nanoparticles exhibit differential modes of toxic and oxidative action in hemocytes of mussel Mytilus galloprovincialis

Cited by 18 publications

References 83 publications

Zinc Oxide Nanoparticles Prime a Protective Immune Response in Galleria mellonella to Defend Against Candida albicans

Zinc Oxide Nanoparticles Prime a Protective Immune Response in Galleria mellonella to Defend Against Candida albicans

The Influence of Silver Nanoparticle Form on the Toxicity in Freshwater Mussels

Aquatic organisms modulate the bioreactivity of engineered nanoparticles: focus on biomolecular corona

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