ZnO nanoparticles (ZnO-NPs) and their antifungal activity against coffee fungus Erythricium salmonicolor

Abstract

In this work, a methodology of synthesis was designed to obtain ZnO nanoparticles (ZnO NPs) in a controlled and reproducible manner. The nanoparticles obtained were characterized using infrared spectroscopy, X-ray diffraction, and transmission electron microscopy (TEM). Also, we determined the antifungal capacity in vitro of zinc oxide nanoparticles synthesized, examining their action on Erythricium salmonicolor fungy causal of pink disease. To determine the effect of the quantity of zinc precursor used during ZnO NPs synthesis on the antifungal capacity, 0.1 and 0.15 M concentrations of zinc acetate were examined. To study the inactivation of the mycelial growth of the fungus, different concentrations of ZnO NPs of the two types of synthesized samples were used. The inhibitory effect on the growth of the fungus was determined by measuring the growth area as a function of time. The morphological change was observed with high-resolution optical microscopy (HROM), while TEM was used to observe changes in its ultrastructure. The results showed that a concentration of 9 mmol L -1 for the sample obtained from the 0.15 M and at 12 mmol L -1 for the 0.1 M system significantly inhibited growth of E. salmonicolor. In the HROM images a deformation was observed in the growth pattern: notable thinning of the fibers of the hyphae and a clumping tendency. The TEM images showed a liquefaction of the cytoplasmic content, making it less electron-dense, with the presence of a number of vacuoles and significant detachment of the cell wall.

Application of ZnO-nanoparticles to manage Rhizopus soft rot of sweet potato and prolong shelf-life

“…Based on these and our findings, we suggest that the use of ZnO-NPs in active packaging could be a viable alternative to the common technologies for improving the shelf-life of sweet potato tubers. Although the action of zinc oxide on fungal growth and enzyme activities may be attributed to the effects of ZnO-NPs on chitin and glucan, as reported by Arciniegas-Grijalba et al (2017), who recorded a noticeable thickening of the cell wall and liquefaction of the cytoplasmic contents of Erythricium salmonicolor. Consequently, ZnO-NPs might control or reduce the synthesis of chitin and glucan enzymes (Romero et al, 2005;Merzendorfer, 2006).…”

ZnO Nanostructures for Drug Delivery and Theranostic Applications

“…The antifungal effect of ZnO QDs has also been proven against other plant pathogens [ 98 , 99 , 100 ].…”

Zeolite/ZnO composites based on a Cuban natural clinoptilolite and preliminary evaluation in methylene blue adsorption

“…They can possess physical properties such as uniformity, conductance or special optical properties that make them desirable in materials science and biology. ZnO nanoparticles (ZnO NPs) are been widely explored due to their antifungal, catalyst and adsorbent properties [9][10][11]. Recently, ZnO NPs have been evaluated in CH 4 and CO 2 separation and adsorption [12].…”