ZnO Nanorods Coated Tapered U-Shape Plastic Optical Fiber for Relative Humidity Detection

Johari, Siti Halma; Tiu, Zian Cheak; Rahim, Hazli Rafis Abdul; Jali, Mohd Hafiz; Yusof, Haziezol Helmi Mohd; Johari, Megat Azmi Megat; Yasin, Moh.; Harun, Sulaiman Wadi

doi:10.3390/photonics9110796

Cited by 4 publications

(2 citation statements)

References 46 publications

(57 reference statements)

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“…Of note is the work of Johari et al (2022) [ 24 ], who used a tapered U-Shaped POF sensor coated with ZnO nanorods to measure relative humidity, and the work of Hadi and Khurshid (2022) [ 25 ], who used a U-Shaped POF immunosensor for the detection of SARS-CoV-2.…”

Section: Introductionmentioning

confidence: 99%

Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacterial Detection in Water

Lopes,

Pinto,

Vargas

et al. 2024

Polymers

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Conventional methods for pathogen detection in water rely on time-consuming enrichment steps followed by biochemical identification strategies, which require assay times ranging from 24 hours to a week. However, in recent years, significant efforts have been made to develop biosensing technologies enabling rapid and close-to-real-time detection of waterborne pathogens. In previous studies, we developed a plastic optical fiber (POF) immunosensor using an optoelectronic configuration consisting of a U-Shape probe connected to an LED and a photodetector. Bacterial detection was evaluated with the immunosensor immersed in a bacterial suspension in water with a known concentration. Here, we report on the sensitivity of a new optoelectronic configuration consisting of two POF U-shaped probes, one as the reference and the other as the immunosensor, for the detection of Escherichia coli. In addition, another methos of detection was tested where the sensors were calibrated in the air, before being immersed in a bacterial suspension and then read in the air. This modification improved sensor sensitivity and resulted in a faster detection time. After the immunocapture, the sensors were DAPI-stained and submitted to confocal microscopy. The histograms obtained confirmed that the responses of the immunosensors were due to the bacteria. This new sensor detected the presence of E. coli at 104 CFU/mL in less than 20 min. Currently, sub-20 min is faster than previous studies using fiber-optic based biosensors. We report on an inexpensive and faster detection technology when compared with conventional methods.

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Section: Introductionmentioning

confidence: 99%

Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacterial Detection in Water

Lopes,

Pinto,

Vargas

et al. 2024

Polymers

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show abstract

“…Also worth mentioning is the work of Johari et al (2022) [24], who used a tapered U-Shape POF sensor coated with ZnO nanorods to measure relative humidity, and the work of Hadi and Khurshid (2022) [25], who used a U-Shape POF immunosensor for the detection of SARS-CoV-2.…”

Section: Introductionmentioning

confidence: 99%

Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacteria Detection

Lopes,

Pinto,

Vargas

et al. 2024

Preprint

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Conventional methods for pathogen detection in water rely on time-consuming enrichment steps followed by biochemical identification strategies, which require assay times ranging from 24 hours to up to a week. However, in recent years, significant efforts have been made towards the development of biosensing technologies enabling rapid and close-to-real-time detection of waterborne pathogens. In previous studies, we developed a plastic optical fiber (POF) immunosensor using an optoelectronic configuration consisting of a U-Shape probe connected to an LED and a photodetector. Bacterial detection was evaluated with the immunosensor immersed in a bacterial suspension in water with a known concentration. Here, we report on the sensitivity of a new optoelectronic configuration consisting of two POF U-Shape probes, one as the reference and the other as the immunosensor, for the detection of Escherichia coli. In addition, another way of detection was tested where the sensors were calibrated in the air, before immersed in bacterial suspension and then read in the air, making the immunosensor more sensitive and with a faster detection time. This new sensor detected the presence of E. coli at 104 CFU/mL in less than 10 minutes. Currently sub 10 minutes is faster than previous studies using fiber-optic based biosensors. It is also a much simpler and quicker methodology when compared with conventional laboratory technology.

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High-sensitivity humidity sensor based on Mach-Zehnder interferometer in seven-core fiber

Shao,

Yu,

et al. 2025

Optics Communications

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ZnO Nanorods Coated Tapered U-Shape Plastic Optical Fiber for Relative Humidity Detection

Cited by 4 publications

References 46 publications

Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacterial Detection in Water

Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacterial Detection in Water

Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacteria Detection

High-sensitivity humidity sensor based on Mach-Zehnder interferometer in seven-core fiber

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