ZntR is a Zn(II)‐responsive MerR‐like transcriptional regulator of <i>zntA</i> in <i>Escherichia coli</i>

Brocklehurst, Kathryn R.; Hobman, Jon L.; Lawley, Blair; Blank, Lindsay; Marshall, Samantha J.; Brown, Nigel L.; Morby, Andrew P.

doi:10.1046/j.1365-2958.1999.01229.x

Cited by 237 publications

(253 citation statements)

References 40 publications

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“…6). During the course of this work, a MerR-like protein from E. coli has been shown to activate transcription from the zntA operator-promoter in response to zinc (41), and a similar activity suggested for a homologue from Proteus mirabilis (42). It will be intriguing to determine how/if responses of ZntR are modified coincident with fluctuating requirements for zinc.…”

Section: Discussionmentioning

confidence: 77%

Cobalt-dependent Transcriptional Switching by a Dual-effector MerR-like Protein Regulates a Cobalt-exporting Variant CPx-type ATPase

Rutherford¹,

Cavet

Robinson

1999

Journal of Biological Chemistry

118

151

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CoaR associates with and confers cobalt-dependent activation of the coaT operator-promoter. A CoaR mutant (Ser-Asn-Ser) in a carboxyl-terminal Cys-His-Cys motif bound the coaT operator-promoter but did not activate expression in response to cobalt, implicating thiolate and/or imidazole ligands at these residues in an allosteric cobalt binding site. Deletion of 1 or 2 nucleotides from between near consensus, but with aberrant (20 base pairs) spacing, ؊10 and ؊35 elements enhanced expression from the coaT operator-promoter but abolished activation by cobalt-CoaR. It is inferred that cobalt effects a transition in CoaR that underwinds the coaT operator-promoter to realign promoter elements. In the absence of cobalt, CoaR represses expression (ϳ50%). CoaR is a fusion of ancestral MerR (mercuryresponsive transcriptional activator)-and precorrin isomerase (enzyme of vitamin B 12 biosynthesis)-related sequences.Expression from the coaT operator-promoter was enhanced in a partial mutant of cbiE (encoding an enzyme preceding precorrin isomerase in B 12 biosynthesis), revealing that this pathway "inhibits" coaT expression. Disruption of coaT reduced cobalt tolerance and increased cytoplasmic 57

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Section: Discussionmentioning

confidence: 77%

Cobalt-dependent Transcriptional Switching by a Dual-effector MerR-like Protein Regulates a Cobalt-exporting Variant CPx-type ATPase

Rutherford¹,

Cavet

Robinson

1999

Journal of Biological Chemistry

118

151

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“…CueR is the transcriptional regulator of CopA. CueR is a member of the MerR family of transcriptional regulators, which occur in a range of bacterial genera and respond to a wide variety of external stimuli (Amábile-Cuevas & Demple, 1991;Brocklehurst et al, 1999;Chiu et al, 1999;Stoyanov et al, 2001;Summers, 1992). CopA is a copper-transporting P-type ATPase efflux pump and a central component of copper homeostasis in E. coli (Rensing et al, 2000).…”

Section: Relative Genomic Contexts Of the Pig Clustersmentioning

confidence: 99%

The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation

et al. 2004

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The prodigiosin biosynthesis gene cluster (pig cluster) from two strains of Serratia (S. marcescens ATCC 274 and Serratia sp. ATCC 39006) has been cloned, sequenced and expressed in heterologous hosts. Sequence analysis of the respective pig clusters revealed 14 ORFs in S. marcescens ATCC 274 and 15 ORFs in Serratia sp. ATCC 39006. In each Serratia species, predicted gene products showed similarity to polyketide synthases (PKSs), non-ribosomal peptide synthases (NRPSs) and the Red proteins of Streptomyces coelicolor A3(2). Comparisons between the two Serratia pig clusters and the red cluster from Str. coelicolor A3(2) revealed some important differences. A modified scheme for the biosynthesis of prodigiosin, based on the pathway recently suggested for the synthesis of undecylprodigiosin, is proposed. The distribution of the pig cluster within several Serratia sp. isolates is demonstrated and the presence of cryptic clusters in some strains shown. The pig cluster of Serratia marcescens ATCC 274 is flanked by cueR and copA homologues and this configuration is demonstrated in several S. marcescens strains, whilst these genes are contiguous in strains lacking the pig cluster.

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“…In Escherichia coli (Brocklehurst et al, 1999;Outten et al, 2001), Bacillus subtilis (Gaballa and Helmann, 1998;Gaballa et al, 2002) and Saccharomyces cerevisiae (Zhao and Eide, 1997;Jamison McDaniels et al, 1999;Daniels et al, 2002;Bird et al, 2003) a large number of genes were identified encoding metallo-regulatory proteins that act as zinc sensors and transcriptional activators and/or repressors. Zinc finger-containing transcription factors not only regulate 'zinc-sensitive' genes, but also control their own transcription through a positive autoregulatory mechanism (Lyons et al, 2000).…”

Section: Zinc Participates In Gene Expression Controlmentioning

confidence: 99%

Nutrient-gene interactions: a single nutrient and hundreds of target genes

Daniel¹,

Dieck²

2004

Biological Chemistry

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Based on the effects of a selective experimental zinc deficiency in a rodent model we explore the use of transcriptome profiling for assessing nutrient-gene interactions in the liver at the molecular and cellular levels. Zinc deficiency caused pleiotropic alterations in mRNA/protein levels of hundreds of genes. In the context of observed metabolic alterations in hepatic metabolism, possible mechanisms are discussed for how a low zinc status may be sensed and transmitted into changes in various metabolic pathways. However, it also becomes obvious that analysis of such complex nutrient-gene interactions beyond the descriptional level is a real challenge for systems biology.

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ZntR is a Zn(II)‐responsive MerR‐like transcriptional regulator of zntA in Escherichia coli

Cited by 237 publications

References 40 publications

Cobalt-dependent Transcriptional Switching by a Dual-effector MerR-like Protein Regulates a Cobalt-exporting Variant CPx-type ATPase

Cobalt-dependent Transcriptional Switching by a Dual-effector MerR-like Protein Regulates a Cobalt-exporting Variant CPx-type ATPase

The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation

Nutrient-gene interactions: a single nutrient and hundreds of target genes

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