ZO1 in Corneal Epithelium: Association to the Zonula Occludens and Adherens Junctions

Sugrue, Stephen P.; Zieske, James D.

doi:10.1006/exer.1996.0175

Cited by 127 publications

(79 citation statements)

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“…One might speculate that further dissolution of the fibrin layer will occur only slowly, once the tight junctions are present between the epithelial cells, forming an impermeable barrier. 35 In the fibrin glue group, we observed no clinical or histopathologic evidence of adverse effects to epithelium, keratocytes, or endothelium. In contrast, in the suture group a mild inflammatory reaction was detected adjacent to intrastromal sutures.…”

Section: Discussionmentioning

confidence: 88%

Sutureless Amniotic Membrane Fixation Using Fibrin Glue for Ocular Surface Reconstruction in a Rabbit Model

Szurman

Warga²,

Grisanti³

et al. 2006

Cornea

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Our results demonstrate the general feasibility of reproducible and reliable sutureless amniotic membrane fixation onto the corneal surface in rabbits. Stable adherence is maintained until epithelialization is completed. The sutureless technique gives sufficient manipulation time for the sheet before the final cross-linking process is completed. Furthermore, several advantageous characteristics could be demonstrated as increased biocompatibility, better epithelialization pattern and the lack of membrane shrinkage.

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Section: Discussionmentioning

confidence: 88%

Sutureless Amniotic Membrane Fixation Using Fibrin Glue for Ocular Surface Reconstruction in a Rabbit Model

Szurman

Warga²,

Grisanti³

et al. 2006

Cornea

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“…Thus, we use the term DNcad hereafter for simplicity. DN-cad was electroporated into the neural tube of Hamburger-Hamilton stage 14 (HH14) chick embryos and the disruption of apical junctions was examined after staining for ZO-1, a protein typically localized to AJs and TJs (Sugrue and Zieske, 1997; supplementary material Fig. S5C).…”

Section: Impact Of Junction Disruption On Neurogenesismentioning

confidence: 99%

Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates

et al. 2014

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The development of the vertebrate brain requires an exquisite balance between proliferation and differentiation of neural progenitors. Notch signaling plays a pivotal role in regulating this balance, yet the interaction between signaling and receiving cells remains poorly understood. We have found that numerous nascent neurons and/or intermediate neurogenic progenitors expressing the ligand of Notch retain apical endfeet transiently at the ventricular lumen that form adherens junctions (AJs) with the endfeet of progenitors. Forced detachment of the apical endfeet of those differentiating cells by disrupting AJs resulted in precocious neurogenesis that was preceded by the downregulation of Notch signaling. Both Notch1 and its ligand Dll1 are distributed around AJs in the apical endfeet, and these proteins physically interact with ZO-1, a constituent of the AJ. Furthermore, live imaging of a fluorescently tagged Notch1 demonstrated its trafficking from the apical endfoot to the nucleus upon cleavage. Our results identified the apical endfoot as the central site of active Notch signaling to securely prohibit inappropriate differentiation of neural progenitors.

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“…[2][3][4] However, for topically applied drugs to be effective, the compounds need to penetrate the cornea. The corneal epithelium consists of 5 to 6 layers of columnar epithelial cells that form tight junctions, 5 limiting paracellular diffusion. Since a large number of naturally occurring nucleoside and nucleoside analogs are hydrophilic, 6 transcellular diffusion is also low, thereby limiting the effectiveness of these antiviral agents.…”

Section: Introductionmentioning

confidence: 99%

Functional differences in nucleoside and nucleobase transporters expressed on the rabbit corneal epithelial cell line (SIRC) and isolated rabbit cornea

et al. 2003

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ABSTRACTclovir permeates the cornea primarily by passive diffusion. Nucleoside and nucleobase transporters on rabbit cornea and corneal epithelial cell line, SIRC, are functionally different, undermining the utility of the SIRC cell line as an in vitro screening tool for elucidating the corneal permeation mechanism of nucleoside analogs. Uptake and transport studies were performed for 2 minutes and 120 minutes, respectively, at 34°C. Thymidine uptake by SIRC displayed saturable kinetics (K m = 595.9 ± 80.4μM, and V max = 289.5 ± 17.2 pmol/min/mg protein). Uptake was inhibited by both purine and pyrimidine nucleosides but not by nucleobases. [ 3 H]thymidine uptake was sodium and energy independent but was inhibited by nitrobenzylthioinosine at nanomolar concentrations. Adenine uptake by SIRC consisted of a saturable component (K m = 14.4 ± 2.3μM, V max = 0.4 ± 0.04 nmol/min/mg protein) and a nonsaturable component. Uptake of adenine was inhibited by purine nucleobases but not by the nucleosides or pyrimidine nucleobases and was independent of sodium, energy, and nitrobenzylthioinosine. [ 3 H]ganciclovir uptake involved a carrier-mediated component and was inhibited by the purine nucleobases but not by the nucleosides or pyrimidine nucleobases. However, transport of [ 3 H]adenine across the isolated rabbit cornea was not inhibited by unlabeled adenine. Further, corneal permeability of ganciclovir across a 100-fold concentration range remained constant, indicating that ganci-

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ZO1 in Corneal Epithelium: Association to the Zonula Occludens and Adherens Junctions

Cited by 127 publications

References 0 publications

Sutureless Amniotic Membrane Fixation Using Fibrin Glue for Ocular Surface Reconstruction in a Rabbit Model

Sutureless Amniotic Membrane Fixation Using Fibrin Glue for Ocular Surface Reconstruction in a Rabbit Model

Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates

Functional differences in nucleoside and nucleobase transporters expressed on the rabbit corneal epithelial cell line (SIRC) and isolated rabbit cornea

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