Zoledronic Acid Induces Site-Specific Structural Changes and Decreases Vascular Area in the Alveolar Bone

Soares, Mariana Quirino Silveira; Dessel, Jeroen Van; Jacobs, Reinhilde; Santos, Paulo Sérgio da Silva; Cestari, Tânia Mary; Garlet, Gustavo; Duarte, Marco Antônio Húngaro; Imada, Thaís Sumie Nozu; Lambrichts, Ivo; Rubira-Bullen, Izabel Regina Fischer

doi:10.1016/j.joms.2018.03.007

Cited by 27 publications

(23 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Three‐dimensional (3D) analysis of the bone structure became feasible with the introduction of microcomputed tomography (micro‐CT), which allows bone morphometry quantification with parameters originally developed in histomorphometry (Van Dessel et al, ). This method objectively shows structure bone changes after a specific drug treatment (Borah et al, ), and differences between healthy and osteoporotic bone (McDonnell, McHugh, & O'Mahoney, ; Soares et al, ). In implantology, trabecular bone morphometric parameters were shown to be associated with implant stability (Kang et al, ; Ribeiro‐Rotta et al, ), indicating its importance for bone quality assessment before implant placement.…”

Section: Introductionmentioning

confidence: 99%

Relationship between trabecular bone architecture and early dental implant failure in the posterior region of the mandible

Nicolielo

Dessel

Jacobs

et al. 2019

Clinical Oral Implants Res

Self Cite

View full text Add to dashboard Cite

Objective: To investigate the relationship between preoperative trabecular bone structure and implant outcome based on bone morphometric bone parameters from CBCT scans. Materials and Methods:Twenty consecutive cases with early implant failure in the posterior region of the mandible were matched with 20 control patients with a successful implant osseointegration selected. All patients had taken a preoperative CBCT image according to a standardized acquisition protocol. On these CBCT scans, the trabecular bone of each implantation site was selected and segmented, after which 3D morphometric bone parameters were calculated and used in a cluster analysis to objectively differentiate trabecular bone patterns. Fisher's exact test was used to determine whether there is a significant association between trabecular pattern and implant outcome. Results: A sparse, intermediate, and dense trabecular bone pattern was distinguished by cluster analysis. The relationship between the trabecular bone pattern and early implant failure was significant (z = 9.6; p < .05). Early implant failure was more likely to occur in the sparse bone types, while implant survival was associated with intermediate bone types. Conclusion: Prior to implant placement, attention should be given to extreme deviations in trabecular structure at the planned implant sites. Very sparse or very dense bone should be carefully evaluated at the potential implant site, while intermediate bone types seem favorable for implant survival. K E Y W O R D Scone-beam CT, dental implant, implant survival, mandible, trabecular bone

show abstract

Section: Introductionmentioning

confidence: 99%

Relationship between trabecular bone architecture and early dental implant failure in the posterior region of the mandible

Nicolielo

Dessel

Jacobs

et al. 2019

Clinical Oral Implants Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…20 The socket of animals in the SZA group corticalization and decrease vascularization in the jaw. 29 Regarding the molecular analysis, focused on the RANK/RANKL/OPG system, RANK and RANKL expression increased and OPG decrease in the animals in the SZA group, suggesting a greater activation of the osteoclasts. 30 These results corroborate those found by Di Nisio,et al 31 2015 In this context, the activation of osteoclasts could be a protective mechanism of the bone tissue to delimit the necrotic area and eliminate infection.…”

Section: Discussionmentioning

confidence: 99%

Delayed alveolar bone repair and osteonecrosis associated with Zoledronic Acid therapy in rats: macroscopic, microscopic and molecular analysis

et al. 2020

Self Cite

View full text Add to dashboard Cite

Objective: This study aims to evaluate bone repair and the development of the medication related osteonecrosis of the jaw (MRONJ) associated with the use of zoledronic acid in Wistar rats. Methodology: 48 male Wistar rats were divided into four groups: ZA, treated with intraperitoneal zoledronic acid, 0.6 mg/kg every 28 days, totaling five doses; control (C), treated with 0.9% sodium chloride; ZA-surgical (SZA) and C-surgical (SC), submitted to extraction of the right upper molars 45 days after the first application. Alveolar bone repair was evaluated by macroscopic and histological analysis. Protein expression evaluations were performed by qPCR. Results: Macroscopic evaluation showed that 91.66% (11) of the animals in the SZA group and 41.66% (5) from the SC group presented solution of epithelium continuity (P<0.05). All animals in the SZA group and none in the SC group had bone sequestration. The area of osteonecrosis was higher in the SZA group than in the SC group (P<0.05). In molecular evaluation, the SZA group presented changes in the expression of markers for osteoclasts, with increased RANK and RANKL, and a decrease in OPG. Conclusion: The results highlighted strong and evident interference of zoledronic acid in bone repair of the socket, causing osteonecrosis and delayed bone remodeling.

show abstract

“…At the end of the 10th week, bilateral extraction of the lower first and second molars was performed in each rat under general anesthesia (1.5%-2% isoflurane in 100% oxygen) to simulate a bisphosphonate-related osteonecrosis of the jaw (BRONJ)-associated bone defect. 27,28 Based on the genetic differences between humans and rodents, a 10-week ZA delivery period with the mentioned dosage frequency along with tooth extraction is known to be capable of effectively replicating a BRONJ-associated bone defect. 29 ZA was delivered for an additional 10 weeks after tooth extraction so that follow-up changes in the region of the bone defect could be observed.…”

Section: Methodsmentioning

confidence: 99%

“…The histological sections were manually registered and spatially aligned with the corresponding reconstructed micro-CT and HR-MRI images for evaluation of the same region of interest. 28 Thereafter, histological assessment of blood vessels was performed using a light microscope (Axio Imager M2; Carl Zeiss Microscopy, Jena, Germany).…”

Section: Histological Analysismentioning

confidence: 99%

“…We observed localized mineralization and a reduction in vascularization with smaller marrow spaces and narrow canals, which were in accordance with the previous findings. 28,42,43 It should be mentioned that based on the small sample size, significant evidence regarding the interaction of bone vascularization and remodeling could not be found. Further prospective studies utilizing a similar methodology should thus be carried out with a larger sample size to confirm our findings.…”

Section: A B C Dmentioning

confidence: 99%

See 1 more Smart Citation

In vivo quantification of mandibular bone remodeling and vascular changes in a Wistar rat model: A novel HR-MRI and micro-CT fusion technique

et al. 2020

Self Cite

View full text Add to dashboard Cite

Purpose: This study was performed to introduce an in vivo hybrid multimodality technique involving the coregistration of micro-computed tomography (micro-CT) and high-resolution magnetic resonance imaging (HR-MRI) to concomitantly visualize and quantify mineralization and vascularization at follow-up in a rat model. Materials and Methods: Three adult female rats were randomly assigned as test subjects, with 1 rat serving as a control subject. For 20 weeks, the test rats received a weekly intravenous injection of 30 μg/kg zoledronic acid, and the control rat was administered a similar dose of normal saline. Bilateral extraction of the lower first and second molars was performed after 10 weeks. All rats were scanned once every 4 weeks with both micro-CT and HR-MRI. Micro-CT and HR-MRI images were registered and fused in the same 3-dimensional region to quantify blood flow velocity and trabecular bone thickness at T0 (baseline), T4 (4 weeks), T8 (8 weeks), T12 (12 weeks), T16 (16 weeks), and T20 (20 weeks). Histological assessment was the gold standard with which the findings were compared. Results: The histomorphometric images at T20 aligned with the HR-MRI findings, with both test and control rats demonstrating reduced trabecular bone vasculature and blood vessel density. The micro-CT findings were also consistent with the histomorphometric changes, which revealed that the test rats had thicker trabecular bone and smaller marrow spaces than the control rat. Conclusion: The combination of micro-CT and HR-MRI may be considered a powerful non-invasive novel technique for the longitudinal quantification of localized mineralization and vascularization.

show abstract

Zoledronic Acid Induces Site-Specific Structural Changes and Decreases Vascular Area in the Alveolar Bone

Cited by 27 publications

References 38 publications

Relationship between trabecular bone architecture and early dental implant failure in the posterior region of the mandible

Relationship between trabecular bone architecture and early dental implant failure in the posterior region of the mandible

Delayed alveolar bone repair and osteonecrosis associated with Zoledronic Acid therapy in rats: macroscopic, microscopic and molecular analysis

In vivo quantification of mandibular bone remodeling and vascular changes in a Wistar rat model: A novel HR-MRI and micro-CT fusion technique

Contact Info

Product

Resources

About