Zona‐free hamster eggs: Their use in assessing fertilizing capacity and examining chromosomes of human spermatozoa

Yanagimachi, Ryuzo

doi:10.1002/mrd.1120100210

Cited by 260 publications

(120 citation statements)

References 152 publications

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“…As a result of the hamster test using the HSA preparation, the penetration rate was 69+9% (mean+s.E.) (range : 33-98%) in the subjects of proven fertility, being higher than the mean 57% (Yanagimachi 1984), with a lowest limit of 33%. The penetration rate in the infertile subjects was 34+5%.…”

Section: Resultsmentioning

confidence: 70%

“…Crystallized and essentially globulin-free HSA is considered to be of higher purity (Kyono et al 1985a). The variation of penetration rates seems to stem from the amount of phospholipid and fatty acids present as contaminant in each albumin preparation (Yanagimachi 1984). As a result of the hamster test using the HSA preparation, the penetration rate was 69+9% (mean+s.E.)…”

Section: Resultsmentioning

confidence: 98%

“…All other subjects showed a penetration rate of over 30%• DISCUSSION Fertilizing capacity testing of human spermatozoa using hamster eggs has Results of the hamster test become clinically necessary. However, the results of the tests cannot be campared simply because of the fact that the conditions used for incubation vary according to the investigator (Hoshi et al 1982a, b ;Yanagimachi 1984). Kyono et al (1985a) reported that the penetration rate of human spermatozoa changes greatly with the type and concentration of HSA preparation in the culture medium.…”

Section: Resultsmentioning

confidence: 99%

“…fertilizing capacity ; zona-free hamster egg ; human spermatozoa ; human serum albumin It was demonstrated by Yanagimachi et al (1976) that acrosome-reacted human spermatozoa having fertilizing capacity can penetrate into a zona-free hamster egg. Since then, this hamster test has been widely used clinically, and shown to be a good procedure for determining the fertilizing capacity of human spermatozoa (Yanagimachi 1984). However, a significant difference in the penetration rate of human spermatozoa due to variations in the conditions used for incubation (particularly, the type and concentration of serum albumin) has been found.…”

mentioning

confidence: 99%

“…Since then, this hamster test has been widely used clinically, and shown to be a good procedure for determining the fertilizing capacity of human spermatozoa (Yanagimachi 1984). However, a significant difference in the penetration rate of human spermatozoa due to variations in the conditions used for incubation (particularly, the type and concentration of serum albumin) has been found.…”

mentioning

confidence: 99%

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A study of the fertilizing capacity of human spermatozoa : The hamster test.

Kyono

1987

Tohoku J. Exp. Med.

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A Study of the Fertilizing Capacity of Human Spermatozoa : The Hamster Test. Tohoku J. exp. Med., 1987, 151(3), [345][346][347][348][349] There are many reports on the hamster test as a test of the fertilizing capacity of human spermatozoa, and its usefulness is widely known. However, the results obtained cannot be simply compared because of the different conditions used for the test. According to our studies, the type of serum albumin present in incubated specimens was considered to affect particularly the rate of sperm penetration. Penetration rates of human spermatozoa were determined under the most appropriate test conditions using good-quality human serum albumin. The penetration rate was 69+9% (range : 33-98%) in men whose fertility had been confirmed within 2 years before test, while it was 34+5% (range : 0-100%) in infertile men, showing a significantly low rate. Eight of nine men who achieved fertilization within one year after the examination showed a penetration rate of over 30%. The remaining one showed a rate of 16%, but he underwent surgery for varicocele and treatment with methylcobalamin and Hochuekkito after the test. Men thus seem difficult to conceive women when the penetration rate of their spermatozoa is below 30%. fertilizing capacity ; zona-free hamster egg ; human spermatozoa ; human serum albumin It was demonstrated by Yanagimachi et al. (1976) that acrosome-reacted human spermatozoa having fertilizing capacity can penetrate into a zona-free hamster egg. Since then, this hamster test has been widely used clinically, and shown to be a good procedure for determining the fertilizing capacity of human spermatozoa (Yanagimachi 1984). However, a significant difference in the penetration rate of human spermatozoa due to variations in the conditions used for incubation (particularly, the type and concentration of serum albumin) has been found. In the present study, a clinical laboratory test was carried out under optimum conditions and the results were compared with those which have been reported so far.The experiment was

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Section: Resultsmentioning

confidence: 70%

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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A study of the fertilizing capacity of human spermatozoa : The hamster test.

Kyono

1987

Tohoku J. Exp. Med.

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Sea urchin sperm nuclear enlargement and shape transformations are differentially regulated in vitro

Raskin

Wright

1997

J. Exp. Zool.

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To elucidate the mechanisms regulating morphogenesis of the sperm nucleus into a male pronucleus after fertilization, we have used an in vitro cell‐free system derived from sea urchin eggs. Demembranated sperm nuclei were added to extracts prepared from either unfertilized eggs (unfertilized extract) or fertilized eggs (fertilized extract), and the area and shape of the sperm nuclei were measured using video‐intensified fluorescence microscopy coupled with image analysis. Sperm nuclei did not decondense in buffer used to prepare the extracts, indicating decondensation factors are of maternal origin. Unfertilized extract supported sperm nuclear decondensation to a greater extent than fertilized extract. This was not due to egg activation nor different preparation methods, but was due to the fertilizing sperm nucleus binding or inactivating diffusible decondensation factors within 10 min. Sperm nuclei needed constant exposure to egg cytoplasm for greater than 30 min for decondensation to proceed. Although sperm nuclear decondensation was temperature sensitive, a thermostable (100°C) decondensing factor was present in egg cytoplasm in limited quantities. Sperm nuclear decondensation was ATP‐dependent, pH‐sensitive, and required kinase activity, including tyrosine kinase activity. Shape transformations in fertilized extract were insensitive to 6‐dimethylaminopurine indicating the area and shape alterations are regulated differentially. In addition, alterations of shape, but not area, were sensitive to serine protease inhibitors, suggesting proteases change the sperm nuclear matrix, thereby altering shape. Sperm nuclear decondensation factors and their targets were highly conserved between sea urchin species, demonstrating a common decondensing mechanism is utilized, which may be applicable to other species. J. Exp. Zool. 277:401–416, 1997. © 1997 Wiley‐Liss, Inc.

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Role of phospholipase A2 in mammalian sperm-egg fusion: Development of hamster oolemma fusibility by lysophosphatidylcholine

Riffo

Párraga

1997

J. Exp. Zool.

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Zona‐free hamster eggs: Their use in assessing fertilizing capacity and examining chromosomes of human spermatozoa

Cited by 260 publications

References 152 publications

A study of the fertilizing capacity of human spermatozoa : The hamster test.

A study of the fertilizing capacity of human spermatozoa : The hamster test.

Sea urchin sperm nuclear enlargement and shape transformations are differentially regulated in vitro

Role of phospholipase A2 in mammalian sperm-egg fusion: Development of hamster oolemma fusibility by lysophosphatidylcholine

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