Zona-free hamster ovum penetration by human spermatozoa : influence of
various sperm factors

Courtot, Anne-Marie; Jouannet, Pierre

doi:10.1051/rnd:19850404

Cited by 7 publications

(1 citation statement)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, I support what John Aitken was saying: if we want to recommend some procedure as a standard screening test, I think the swim-up technique with one centrifugation step is the best and the simplest way to isolate the motile spermatozoa. Although centrifugation of the upper layer is not necessary to collect motile sperm and to obtain a good penetration rate (Courtot & Jouannet 1985), it is a useful precaution in order to eliminate seminal plasma which may be aspirated with the supernatant during the procedure of overlay collection.…”

Section: Jouannetmentioning

confidence: 99%

Methodology Sperm preparation techniques

1986

Int J of Andrology

View full text Add to dashboard Cite

Section: Jouannetmentioning

confidence: 99%

Methodology Sperm preparation techniques

1986

Int J of Andrology

View full text Add to dashboard Cite

In vitro fertilization of hamster and human oocytes by microinjection of human sperm

1987

View full text Add to dashboard Cite

In this study, swollen sperm heads were obtained after the injection of human sperm into the perivitelline space of hamster oocytes. The number of injected sperm and the sperm concentration in the preincubation medium were found to have an influence on the rate of penetrated hamster oocytes. The optimal injected sperm number was always between five to 12 to obtain 8, 37, and 36% penetration for donors A, B, and C, respectively. The optimal sperm concentration in preincubation medium was between 6 and 22 x 10(6) sperm/ml to obtain 16, 47, and 43% penetration for donors A, B, and C, respectively. The rate of polyspermic oocytes was related to the injected sperm number (0, 55, and 100% for one to four, five to 12, and more than 12 injected sperm respectively). Ten human mature oocytes were injected with the sperm from six normal donors. Five fertilized eggs were obtained, and of these four cleaved in in vitro culture.

show abstract

Impaired ability of human spermatozoa to penetrate zona‐free hamster oocytes: Is a postacrosomal sheath anomaly involved?

et al. 1987

Self Cite

View full text Add to dashboard Cite

We selected 17 infertile men whose sperm ultrastructural study revealed at least 70% of spermatozoa with postacrosomal sheath (PAS) anomalies. Among the other sperm head defects, those affecting the nuclear shape were most frequently encountered and were highly correlated with PAS anomalies (r = +0.71; P less than .01). PAS anomalies were also correlated with chromatin condensation defects (r = +0.67; P less than .01) and acrosome anomalies (r = +0.53; P less than .05). Those spermatozoa were tested for their ability to penetrate zona-free hamster oocytes and were compared to a control sperm population. It was shown that sperm head morphological anomalies impaired the ability of spermatozoa to attach to and penetrate the oocyte. The highest significant and negative correlations were found between the penetration rate and 1) the percentage of spermatozoa with PAS anomalies (r = -0.81; P less than .01) and 2) the percentage of spermatozoa with nuclear shape anomalies (r = -0.66; P less than .01). The effect of PAS anomalies on human fertilization process are discussed.

show abstract

Zona-free hamster ovum penetration by human spermatozoa : influence of various sperm factors

Cited by 7 publications

References 16 publications

Methodology Sperm preparation techniques

Methodology Sperm preparation techniques

In vitro fertilization of hamster and human oocytes by microinjection of human sperm

Impaired ability of human spermatozoa to penetrate zona‐free hamster oocytes: Is a postacrosomal sheath anomaly involved?

Contact Info

Product

Resources

About