Zona pellucida resistance to sperm penetration before the completion of human oocyte maturation

Tesařík, Jan; Pilka, L; Trávník, Pavel

doi:10.1530/jrf.0.0830487

Cited by 61 publications

(34 citation statements)

References 13 publications

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“…Virtually all spermatozoa associated with the zona pellucida of the antiserum-pretreated oocytes were localized on the surface of the zona pellucida, while almost one-third of zona-associated spermatozoa in the control oocytes had at least begun zona penetration (Table 1). The proportion of spermatozoa within the zona pellucida of the control oocytes was similar to that previously observed for metaphase II oocytes inseminated in vitro using a standard in-vitro fertilization protocol (Tesarik et al, 1988b Effect of anti-cumulus matrix antiserum on cumulus matrix-induced acrosome reaction When spermatozoa were preincubated in capacitating medium for 5 or 16 h and then incubated with the solubilized cumulus matrix, the percentages of acrosome-reacted spermatozoa (evaluated only for living sperm populations) were significantly (P < 001) increased above the values ascertained for sperm samples incubated in the absence of cumulus material (Fig. 1).…”

Section: Effect Of Anti-cumulus Matrix Antiserum On Human Sperm-zona supporting

confidence: 83%

“…The counts of total zona pellucida-associated spermatozoa and the percentages of surface-bound spermatozoa and of those having at least begun zona penetration were determined as previously described (Tesarik et al, 1988b (Tesarik et al, 1988b). In the absence of the zona pellucida, neither the proportion of penetrated oocytes nor the degree of polyspermy were noticeably influenced by the anti-cumulus matrix antibodies (P > 005).…”

Section: Methodsmentioning

confidence: 99%

“…First, the zona pellucida of late preovulatory and early post-ovulatory human oocytes is a dynamic system, into which both the oocyte and cumulus oophorus cells secrete newly synthesized materials (Tesarik & Kopecny, 1986). The materials secreted by the oocyte may have a hardening effect on the human zona pellucida, while those secreted by the cumulus cells apparently cause zona pellucida softening and an increase in its penetrability by homologous spermatozoa (Tesarik et al, 1988b). Anti-cumulus matrix anti¬ bodies may destabilize the equilibrium between these two opposite effects in favour of the oocytesecreted hardening factors and so impede sperm penetration.…”

Section: Effect Of Anti-cumulus Matrix Antiserum On Human Sperm-zona mentioning

confidence: 99%

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Immunoinhibition of human fertilization in vitro by antibodies to the cumulus oophorus intercellular matrix

Tesarik

1989

Reproduction

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Summary. Rabbit polyvalent antiserum raised against the solubilized cumulus matrix was a powerful inhibitor of human fertilization in vitro, affecting sperm\p=n-\zonapellucida interaction. Both sperm binding to, and penetration of, the zona pellucida were severely impaired by the anti\p=n-\cumulusmatrix antiserum, whereas no effects of this antiserum on cumulus matrix solubilization or penetration of zona-free human eggs were evident. Moreover, the anti-cumulus antiserum partly neutralized the acrosome reaction-inducing activity of the cumulus matrix. These results warrant further research into the functional specificity of different cumulus matrix components and into the effects of the respective antibodies on reproductive function as a possible lead to a new approach to contraceptive vaccine development.

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Section: Effect Of Anti-cumulus Matrix Antiserum On Human Sperm-zona supporting

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Section: Effect Of Anti-cumulus Matrix Antiserum On Human Sperm-zona mentioning

confidence: 99%

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Immunoinhibition of human fertilization in vitro by antibodies to the cumulus oophorus intercellular matrix

Tesarik

1989

Reproduction

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“…Notably, scanning electron microscope studies indicate structure of mouse zona pellucidae change during oocyte maturation (Nogues et al, 1988;Calafell et al, 1992), displaying variations in localization, density and distributions of glycoconjugates (Kaufman et al, 1989). Ultrastructural changes in zona pellucida of human oocytes also occur depending on maturational status (Familiari et al, 1988(Familiari et al, , 1989(Familiari et al, , 1992Tesarik et al, 1988). It is likely these changes to surface ultrastructure of zona pellucidae are important in regulating sperm/oocyte binding, perhaps via orientation of sperm-binding sites (Familiari et al, 1988).…”

Section: Sperm/oocyte Binding and Penetrationmentioning

confidence: 99%

ART failure: oocyte contributions to unsuccessful fertilization

Swain

Pool

2008

Human Reproduction Update

244

163

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BACKGROUND: The complexity of fertilization failure during assisted reproductive technologies (ART) is often under-appreciated, as this failure can occur at any number of essential mechanistic and cellular events. Importantly, successful fertilization is heavily dependent upon inherent qualities of the oocytes, and thus reliant upon fidelity of oocyte maturation. METHODS: Pubmed and medline were searched up to April 2008 for papers on oocyte fertilization and its mechanistic components. References to clinical/human studies were selected wherever possible. RESULTS: Successful oocyte maturation cannot simply be determined via visual assessment of polar body extrusion, but rather entails coordination of numerous cytoplasmic processes not readily observed. Proper regulation of intraoocyte signaling cascades is crucial for sufficient production and storage of carbohydrates and proteins, successful relocation of organelles and regulation of metabolic pathways required for an apparently mature metaphase II oocyte to complete subsequent fertilization events; such as cumulus penetration, sperm/oocyte binding, fusion, oocyte activation, sperm processing and pronuclear (PN) formation. Regulation of oocyte maturation begins during oocyte growth and is intimately connected with events influencing folliculogenesis. Therefore, the oocyte is subject to a multitude of potential effector impacting fertilization potential and developmental competence long before encountering the artificial environment of the IVF laboratory. CONCLUSIONS: Although meticulous care and continued research is essential for future improvement, failure to fertilize and properly form PN following clinical ART is likely to be dependent on historical events in oocyte maturation, not easily explained or prevented through simple modification of contemporary laboratory protocols.

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“…Zona maturity 78,98 and proper sperm-zona binding ability have repeatedly been shown to be predictive of successful fertilization in vitro. 99,100 In order to test sperm-zona binding prior to IVF treatment, few zona binding assays have been developed in the past.…”

Section: Zona Pellucida Bindingmentioning

confidence: 99%

Sperm function and assisted reproduction technology

Henkel¹,

Maass²,

Bödeker³

et al. 2005

Reprod Med Biol

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The evaluation of different functional sperm parameters has become a tool in andrological diagnosis. These assays determine the sperm's capability to fertilize an oocyte. It also appears that sperm functions and semen parameters are interrelated and interdependent. Therefore, the question arose whether a given laboratory test or a battery of tests can predict the outcome in in vitro fertilization (IVF).One-hundred and sixty-one patients who underwent an IVF treatment were selected from a database of 4178 patients who had been examined for male infertility 3 months before or after IVF. Sperm concentration, motility, acrosin activity, acrosome reaction, sperm morphology, maternal age, number of transferred embryos, embryo score, fertilization rate and pregnancy rate were determined. In addition, logistic regression models to describe fertilization rate and pregnancy were developed. All the parameters in the models were dichotomized and intra-and interindividual variability of the parameters were assessed. Although the sperm parameters showed good correlations with IVF when correlated separately, the only essential parameter in the multivariate model was morphology. The enormous intra-and interindividual variability of the values was striking. In conclusion, our data indicate that the andrological status at the end of the respective treatment does not necessarily represent the status at the time of IVF. Despite a relatively low correlation coefficient in the logistic regression model, it appears that among the parameters tested, the most reliable parameter to predict fertilization is normal sperm morphology. (Reprod Med Biol 2005; 4: 7-30) Key words: assisted reproduction, high intra-and interindividual variability, multivariate approach, prediction of outcome of IVF, sperm functions.

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Zona pellucida resistance to sperm penetration before the completion of human oocyte maturation

Cited by 61 publications

References 13 publications

Immunoinhibition of human fertilization in vitro by antibodies to the cumulus oophorus intercellular matrix

Immunoinhibition of human fertilization in vitro by antibodies to the cumulus oophorus intercellular matrix

ART failure: oocyte contributions to unsuccessful fertilization

Sperm function and assisted reproduction technology

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