Zonal Differences in DNA Synthesis and in Transglutaminase Activity between Perivenous versus Periportal Regions of Regenerating Rat Liver

Abstract

Hepatocytes are classified into periportal hepatocytes (PPH) and perivenous hepatocytes (PVH) according to studies on a zonal difference in metabolism and proliferation.

The Involvement of Polyamines as Substrates of Transglutaminase in Zonal Different Hepatocyte Proliferation after Partial Hepatectomy

“…1). 21) Although Gln-Lys contents in two subpopulations were same in initial normal liver, they increased transiently in both subpopulations with a peak at different times. In both subpopulations, Gln-Lys contents transiently increased at 1 d in PVH and 2 d in PPH.…”

“…Therefore, at 1 d after partial hepatectomy, relative Gln-Lys content in PVH was 2-times higher than that in PPH, and obtained similar results as in the case for the TGase activities which were previously reported. 21) On the other hand, DNA synthesis in both subpopulations increased transiently with a peak at 1 d after partial hepatectomy, and then declined after following. The peaked amount of DNA synthesis in PPH was twice as much as that in PVH.…”

“…The detailed procedure was described in previous report. 21) Measurement of ODC Activity After the digitonin/collagenase perfusion, the cell suspension was homogenized with 9 volumes of ice-cold buffer (0.25 M sucrose, 10 mM Tris, 2 mM EGTA, 1 mM phenylmethylsulfonylfluoride, pH 7.4) by 15 strokes by a loose-fitting Dounce homogenizer. The homogenate was centrifuged at 105000ϫg for 60 min at 4°C.…”

“…[18][19][20] We recently demonstrated the inverse correlation between DNA synthesis and TGase activity in PPH and PVH at 1 d after partial hepatectomy. 21) These results suggest that TGase is one of many factors which are involved in the regulation of DNA synthesis during liver regeneration. However, it has not been clarified how TGase is concerned with the zonal different hepatocyte proliferation after partial hepatectomy.…”

The Uptake Mechanism of Gallium-67 into Hepatocytes Treated with Carbon Tetrachloride

“…Preparation of Periportal Hepatocytes (PPH) and Perivenous Hepatocytes (PVH) Hepatocytes were obtaind from rat liver using the digitonin/collagenase perfusion technique described by Ohtake et al 29) Briefly, under the anesthetization with urethane, digitonin solution (7 mM) was perfuse from portal vein at a rate of 5 ml/min for 10 to 40 s to sustain better destruction of portal area. After washing out digitonin solution, collagenase was perfused for 2 to 5 min to obtain PVH.…”