Zonisamide: Antiproliferative and Antioxidant Effects on C6 Glioma Cancer Cell Model

Abstract

Seizures are often seen in patients with brain tumors and antiepileptic drugs such as acetazolamide, valproic acid. Some of these antiepileptic drugs also have anti-cancer effects that have been shown in many studies. For instance, valproic acid has been shown to be related to apoptosis pathway and induced the suppression of C6 glioma cell migration. Zonisamide is also commonly used as an antiepileptic drug because of its scavenging effects on hydroxyl and nitric oxide radicals. Due to valproic acid's anticancer activity, zonisamide was investigated for its antiproliferative and antioxidant effects on C6 glioma cells. The percentage of cell viability, measurement of DNA synthesis, superoxide dismutase activity and the measurement of glutathione were studied in the presence of zonisamide. IC 50 value of zonisamide is 80 M at 48 hour. According to DNA synthesis (BrdU incorporation) analysis, IC 50 value and lower concentration (50 M) of zonisamide reduced the rate of C6 glioma DNA synthesis significantly as compared to control cells. Zonisamide was also decreased SOD and GSH levels on glioma C6 cells depending on concentrations and incubation of time. Using of zonisamide with anti-cancer agents fighting against glioma cells might be useful because of its antiproliferative effects.

The Expression of Carbonic Anhydrases II, IX and XII in Brain Tumors

“…As far as we know, there are no major pre-clinical or clinical studies focusing on the effects of zonisamide in brain tumors. One experimental study has demonstrated that zonisamide has antiproliferative effects in C6 rat glioma cells [ 86 ].…”