Zonula occludens-1 (ZO-1) is involved in morula to blastocyst transformation in the mouse

Wang, Hehai; Ding, Tan; Brown, Naoko; Yamamoto, Yoshihito; Prince, Lawrence S.; Reese, Jeff; Paria, Bibhash C.

doi:10.1016/j.ydbio.2008.03.008

Cited by 69 publications

(63 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…65 ZO-1 is a putative adaptor for occludin, claudins, and JAM-A at the BTB. 66 The impact of its deletion on the testis is not known because ZO-1 knockouts are embryonic lethal, 67,68 but the deletion of ZO-2 (a close sibling of ZO-1) in the testis leads to subfertility with a concomitant disruption of the BTB. 69 Deletion of the GJ component Cx43 selectively in Sertoli cells results in spermatogenesis arrest associated with a decrease in spermatogonia and an increase in the number of Sertoli cells per tubule in which spermatocytes failed to develop beyond type B.…”

Section: Discussionmentioning

confidence: 99%

AKAP9, a Regulator of Microtubule Dynamics, Contributes to Blood-Testis Barrier Function

Venkatesh

Mruk

Herter

et al. 2016

The American Journal of Pathology

View full text Add to dashboard Cite

The blood-testis barrier (BTB), formed between adjacent Sertoli cells, undergoes extensive remodeling to facilitate the transport of preleptotene spermatocytes across the barrier from the basal to apical compartments of the seminiferous tubules for further development and maturation into spermatozoa. The actin cytoskeleton serves unique structural and supporting roles in this process, but little is known about the role of microtubules and their regulators during BTB restructuring. The large isoform of the cAMP-responsive scaffold protein AKAP9 regulates microtubule dynamics and nucleation at the Golgi. We found that conditional deletion of Akap9 in mice after the initial formation of the BTB at puberty leads to infertility. Akap9 deletion results in marked alterations in the organization of microtubules in Sertoli cells and a loss of barrier integrity despite a relatively intact, albeit more apically localized F-actin and BTB tight junctional proteins. These changes are accompanied by a loss of haploid spermatids due to impeded meiosis. The barrier, however, progressively reseals in older Akap9 null mice, which correlates with a reduction in germ cell apoptosis and a greater incidence of meiosis. However, spermiogenesis remains defective, suggesting additional roles for AKAP9 in this process. Together, our data suggest that AKAP9 and, by inference, the regulation of the microtubule network are critical for BTB function and subsequent germ cell development during spermatogenesis. (Am J Pathol 2016, 186: 270e284; http://dx

show abstract

Section: Discussionmentioning

confidence: 99%

AKAP9, a Regulator of Microtubule Dynamics, Contributes to Blood-Testis Barrier Function

Venkatesh

Mruk

Herter

et al. 2016

The American Journal of Pathology

View full text Add to dashboard Cite

show abstract

“…The concomitant passage of water forms a fluid-filled blastocoel at the 32-cell stage that defines the early blastocyst (Fig. 1B), and at embryonic day 4.5 (E4.5) the fully formed blastocysts implants into the wall of the uterus (Madan et al, 2007;Wang et al, 2008). Early embryonic development is regulative (see Glossary, Box 1) and cells derived from blastomeres prior to compaction can participate in all tissues of the adult (Tarkowski, 1959;Rossant, 1976).…”

Section: Introductionmentioning

confidence: 99%

Maternal control of early mouse development

2010

View full text Add to dashboard Cite

The hiatus between oocyte and embryonic gene transcription dictates a role for stored maternal factors in early mammalian development. Encoded by maternal-effect genes, these factors accumulate during oogenesis and enable the activation of the embryonic genome, the subsequent cleavage stages of embryogenesis and the initial establishment of embryonic cell lineages. Recent studies in mice have yielded new findings on the role of maternally provided proteins and multi-component complexes in preimplantation development. Nevertheless, significant gaps remain in our mechanistic understanding of the networks that regulate early mammalian embryogenesis, which provide an impetus and opportunities for future investigations.

show abstract

“…(C) Blastocysts used in parallel as positive controls showed specific staining in trophoectoderm cell-cell contact regions. (Ziomek et al, 1980) and the formation of cell-cell adhesion structures such as adherens junctions (Shirayoshi et al, 1983;De Vries et al, 2004), tight junctions (Sheth et al, 1997;Wang et al, 2008) and gap junctions (Houghton et al, 2005). Compaction is followed by the formation of a central cavity, process known as cavitation, and the differentiation of two distinct cell types: the trophoectoderm and the inner cell mass (ICM), to form the blastocyst.…”

Section: Resultsmentioning

confidence: 99%

Life-giving caspases: revealing new roles during mouse embryo preimplantation development

Busso

Domínguez

Pérez-Acle³

et al. 2010

Int. J. Dev. Biol.

View full text Add to dashboard Cite

Caspases, cystein proteases traditionally related to programmed cell death, have recently been found to be involved in vital processes such as cell proliferation, adhesion and differentiation. Although caspases are expressed in mouse embryos before the blastocyst stage, their role is unclear, since apoptosis does not occur significantly before implantation. In this work, we have used mouse preimplantation development as a model to evaluate the existence of nonlethal caspase activities. The use of specific caspase inhibitors during in vitro embryo culture showed that caspase 8 activity, but not caspase 2 or 9, was relevant for development. The inhibition of caspase 8 affected the compaction of morulae and the progression to the blastocyst stage. In agreement with these results, caspase 8 was expressed in mouse embryos, as shown by indirect immunofluorescence and RT-PCR. An in silico approach was used to find putative caspase targets expressed in mouse preimplantation embryos. Large-scale management of sequence data from mouse embryos was used to predict caspase substrates by tools matrix-based on known cleavage sites. A total of 510 potential caspase targets expressed in mouse embryos were identified by this procedure. The functional characterization of these proteins by Gene Onthology associations showed that many of these putative caspase targets were previously related to nonapoptotic functions and only 63 had been previously reported to be actually cleaved by caspases. Interestingly, eleven knockout mice models for caspase substrates identified in our work, i.e. catenin alpha and beta, geminin, pescadillo, calpain-2, have preimplantation lethal phenotypes. This work supports the involvement of caspases in vital functions during mouse preimplantation development and proposes a model in which the regulated cleavage of caspase substrates could account for this role.

show abstract

Zonula occludens-1 (ZO-1) is involved in morula to blastocyst transformation in the mouse

Cited by 69 publications

References 35 publications

AKAP9, a Regulator of Microtubule Dynamics, Contributes to Blood-Testis Barrier Function

AKAP9, a Regulator of Microtubule Dynamics, Contributes to Blood-Testis Barrier Function

Maternal control of early mouse development

Life-giving caspases: revealing new roles during mouse embryo preimplantation development

Contact Info

Product

Resources

About