Zosteric acid and salicylic acid bound to a low density polyethylene surface successfully control bacterial biofilm formation

Cattò, Cristina; James, Garth A.; Villa, Federica; Villa, Stefania; Cappitelli, Francesca

doi:10.1080/08927014.2018.1462342

Cited by 12 publications

(6 citation statements)

References 45 publications

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“…No detectable changes in the wavenumber and the shape of the amide I and amide II bands were observed, suggesting that the ZA treatment did not induce changes in the protein's secondary structure. All these findings-the dramatic changes in carbohydrates, proteins, and DNA profiles over time in the ZAtreated samples-are consistent with the previous results that showed the ability of ZA in hindering cell attachment and in slowing down the biofilm formation on polymeric surfaces (Dell'Orto et al 2017;Cattò et al 2018aCattò et al , 2018b. This finding is a critical aspect in thwarting the biofilm genesis, because once the early biofilm is inhibited, the mature biofilm may in turn be prevented or at least be controlled.…”

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Biochemical and molecular changes of the zosteric acid-treated Escherichia coli biofilm on a mineral surface

et al. 2021

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Purpose The main goal of the present work was to assess the effectiveness of zosteric acid (ZA) in hindering Escherichia coli biofilm formation on a mineral surface. Methods Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) flow system was used to probe in situ the biochemical changes induced by ZA on E. coli sessile cells growing on the zinc selenide ATR plate. Comparative proteome analysis was conducted on the sessile cells to better understand the principal molecular changes that occur on ZA-treated biofilms. Results The ZA treatment modified the kinetics of the biofilm development. After the ZA exposure, dramatic changes in the carbohydrates, proteins, and DNA profiles were observed over time in the ATR-FTIR spectra. These results were translated into the physiological effects such as the reduction of both the biomass and the EPS contents, the inhibition of the biofilm growth, and the promotion of the detachment. In E. coli sessile cells, the comparative proteome analysis revealed that, while the stress responses were upregulated, the pathways belonging to the DNA replication and repair were downregulated in the ZA-treated biofilms. Conclusions The ZA reduced the binding capability of E. coli cells onto the ZnSe crystal, hindering the firm adhesion and the subsequent biofilm development on a mineral surface. The variation of the protein patterns indicated that the ZA acted as a stress factor on the sessile cells that seemed to discourage biomass proliferation, consequently decreasing the surface colonization.

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“…In this context, the use of bioinspired molecules to interfere with the formation of deleterious biofilms in sublethal doses represents an ideal strategy worth pursuing. The new strategy consists of interfering with the key-steps that orchestrate biofilm genesis; thus, the colonization cascade might be hampered (Cattò et al 2018a(Cattò et al , 2018b.…”

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Biochemical and molecular changes of the zosteric acid-treated Escherichia coli biofilm on a mineral surface

et al. 2021

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“…Three-dimensional morphology of biofilm growth on non-functionalized and functionalized surfaces was analyzed by CLSM according to Cattò et al [85]. Biofilm was stained with 200 µg/mL lectin concanavalin A-Texas Red conjugate dye (C825, Molecular Probes-Life Technologies, Thermo Fisher Scientific) to visualize the polysaccharide component of the extracellular polymeric substances (EPS) and 1:1000 SYBR Green I fluorescent nucleic acid dye (S7563, Molecular Probes-Life Technologies, Thermo Fisher Scientific) to display biofilm cells, in the dark for 30 min.…”

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α-Chymotrypsin Immobilized on a Low-Density Polyethylene Surface Successfully Weakens Escherichia coli Biofilm Formation

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James

et al. 2018

IJMS

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The protease α-chymotrypsin (α-CT) was covalently immobilized on a low-density polyethylene (LDPE) surface, providing a new non-leaching material (LDPE-α-CT) able to preserve surfaces from biofilm growth over a long working timescale. The immobilized enzyme showed a transesterification activity of 1.24 nmol/h, confirming that the immobilization protocol did not negatively affect α-CT activity. Plate count viability assays, as well as confocal laser scanner microscopy (CLSM) analysis, showed that LDPE-α-CT significantly impacts Escherichia coli biofilm formation by (i) reducing the number of adhered cells (−70.7 ± 5.0%); (ii) significantly affecting biofilm thickness (−81.8 ± 16.7%), roughness (−13.8 ± 2.8%), substratum coverage (−63.1 ± 1.8%), and surface to bio-volume ratio (+7.1 ± 0.2-fold); and (iii) decreasing the matrix polysaccharide bio-volume (80.2 ± 23.2%). Additionally, CLSM images showed a destabilized biofilm with many cells dispersing from it. Notably, biofilm stained for live and dead cells confirmed that the reduction in the biomass was achieved by a mechanism that did not affect bacterial viability, reducing the chances for the evolution of resistant strains.

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“…Three-D morphology of biofilms grown without and with Er and its Met I at 5 mg/L were analysed by CLSM according to Cattò et al [ 30 ]. Biofilms were stained with 1:1000 SYTO 9 fluorescent nucleic acid dye (excitation 485 nm/498 nm, ThermoFisher, Milan, Italy) and lectin Concanavalin A-Texas Red conjugate (ConA, Thermo Fisher Scientific, Waltham, MA, USA) in the dark for 30 min to display sessile-dwelling cells and the polysaccharide component of the biofilm matrix, respectively.…”

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Understanding the Role of the Antioxidant Drug Erdosteine and Its Active Metabolite on Staphylococcus aureus Methicillin Resistant Biofilm Formation

2021

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Increasing numbers of researches have suggested that some drugs with reactive oxygen species (ROS)-mediated mechanisms of action modulate biofilm formation of some pathogenic strains. However, the full contribution of ROS to biofilm development is still an open question. In this paper, the correlations between the antioxidant drug Erdosteine (Er) and its active Metabolite I (Met I), ROS and biofilm development of two strains of methicillin resistant Staphylococcus aureus are presented. Experiments revealed that Er and Met I at 2 and 5 mg/L increased up to three orders of magnitude the number of biofilm-dwelling cells, while the content of ROS within the biofilms was reduced above the 87%, with a major effect of Met I in comparison to Er. Comparative proteomics showed that, 5 mg/L Met I modified the expression of 30% and 65% of total proteins in the two strains respectively. Some proteins involved in cell replication were upregulated, and a nitric oxide-based mechanism is assumed to modulate the biofilm development by changing quorum sensitive pathways. Additionally, several proteins involved in virulence were downregulated in the presence of Met I, suggesting that treated cells, despite being greater in number, might have lost part of their virulence.

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Zosteric acid and salicylic acid bound to a low density polyethylene surface successfully control bacterial biofilm formation

Cited by 12 publications

References 45 publications

Biochemical and molecular changes of the zosteric acid-treated Escherichia coli biofilm on a mineral surface

Biochemical and molecular changes of the zosteric acid-treated Escherichia coli biofilm on a mineral surface

α-Chymotrypsin Immobilized on a Low-Density Polyethylene Surface Successfully Weakens Escherichia coli Biofilm Formation

Understanding the Role of the Antioxidant Drug Erdosteine and Its Active Metabolite on Staphylococcus aureus Methicillin Resistant Biofilm Formation

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