Zum Mechanismus der Sph�rosomenfluoreszenz mit Oxazinen und anderen basischen Farbstoffen

Mix, Marianne

doi:10.1007/bf01250365

Cited by 18 publications

(4 citation statements)

References 31 publications

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“…The traces of nile red in commercial preparations of nile blue provide the metachromatic coloring of neutral lipids for which the procedure of Smith and Mair ( 9) is known. The synthesis of nile red itself and its direct application as a fluorescent probe for lipids appears not to have been done previously, although there are reports on the fluorescence of nile blue-stained tissues (34)(35)(36). These reports attribute this fluorescence to be possibly due to the presence of nile red in the commercial nile blue dye.…”

Section: Discussionmentioning

confidence: 99%

Nile red: a selective fluorescent stain for intracellular lipid droplets.

Greenspan¹,

Mayer²,

Fowler³

1985

The Journal of Cell Biology

2,262

1,596

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We report that the dye nile red, 9-diethylamino-5H-benzo[a]phenoxazine-5-one, is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry. The specificity of the dye for lipid droplets was assessed on cultured aortic smooth muscle cells and on cultured peritoneal macrophages that were incubated with acetylated low density lipoprotein to induce cytoplasmic lipid overloading. Better selectivity for cytoplasmic lipid droplets was obtained when the cells were viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, > 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, > 590 nm). Nile red-stained, lipid dropletfilled macrophages exhibited greater fluorescence intensity than did nile red-stained control macrophages, and the two cell populations could be differentiated and analyzed by flow cytofluorometry. Such analyses could be performed with either yellow-gold or red fluorescence, but when few lipid droplets per cell were present, the yellow-gold fluorescence was more discriminating.Nile red exhibits properties of a near-ideal lysochrome. It is strongly fluorescent, but only in the presence of a hydrophobic environment. The dye is very soluble in the lipids it is intended to show, and it does not interact with any tissue constituent except by solution. Nile red can be applied to cells in an aqueous medium, and it does not dissolve the lipids it is supposed to reveal.

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Section: Discussionmentioning

confidence: 99%

Nile red: a selective fluorescent stain for intracellular lipid droplets.

Greenspan¹,

Mayer²,

Fowler³

1985

The Journal of Cell Biology

2,262

1,596

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show abstract

“…The admixture of American red wines in others can be detected by the presence of malvidine diglucoside, which has characteristic paper-chromatography properties, including brick-red fluorescence (294) The fluorescence of extracts of Chelidonium majus serves for identification purposes (325). A spherosome fluorescence in leaf epider-mis cells was produced by several oxazine dyes (235). Fluorescent brighteners have applications in biology (73) and elsewhere (394).…”

Section: Magnesiummentioning

confidence: 99%

Fluorometric Analysis

White¹,

Weissler²

1962

Anal. Chem.

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“…DRAWERT 1952u. f., GuTZ 1956, KUTTIG 1957, KARBER 1958, MIX 1959, SAUER 1960 solI iiber Ergebnisse mit den Antibiotica Leucomycin und Tetracyclin berichtet werden, die auf Grund von Versuchsergebnissen anderer Autoren an tierischen Zellen und Bakterien fUr unsere Zwecke geeignet erschienen.…”

unclassified

58. Horst Drawert und Ursula Rüffer‐Bock: Fluorochromierung von Endoplasmatischem Reticulum, Dictyosomen und Chondriosomen mit Tetracyclin

1964

Berichte der Deutschen Botanischen Gesellschaft

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Zum Mechanismus der Sph�rosomenfluoreszenz mit Oxazinen und anderen basischen Farbstoffen

Cited by 18 publications

References 31 publications

Nile red: a selective fluorescent stain for intracellular lipid droplets.

Nile red: a selective fluorescent stain for intracellular lipid droplets.

Fluorometric Analysis

58. Horst Drawert und Ursula Rüffer‐Bock: Fluorochromierung von Endoplasmatischem Reticulum, Dictyosomen und Chondriosomen mit Tetracyclin

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