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Automatized flow-batch method for fluorescent determination of free glycerol in biodiesel samples using on-line extraction

“…Biodiesel is produced by a chemical transesterification reaction, which is a sequence of three reversible chemical reactions to turn triglyceride molecule into diglyceride, monoglyceride and glycerol [2]. Consequently, two phases are obtained; the upper phase that has the main biodiesel product, and the lower phase that consists of glycerol and many other chemical compounds [3].…”