Zur autoradiographischen Markierung von Langerhans-Zellen mit 3H-Thymidin

Schellander, F; Wolff, Klaus

doi:10.1007/bf00496551

Cited by 32 publications

(7 citation statements)

References 8 publications

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“…Using histochemistry to demonstrate leucin-aminopeptidase activity in guinea pigs, Gschnait and Brenner (1979) found a 3.0% LI in normal animals at 40 min after an intracutaneous injection of [3H]TdR whereas 5 days after tape stripping the flash LI was 35%. In comparison, a stimulated LI of 0.01% after tape stripping was reported by Schellander and Wolff (1967).…”

Section: Discussionmentioning

confidence: 82%

DNA synthesis in Langerhans’cells of mouse ear epithelium revealed by tritiated thymidine autoradiography and histochemical staining for non‐specific esterase and beta‐glucuronidase activity

Hume

Moore

1989

Cell Proliferation

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The proportion of Langerhans’cells in DNA synthesis in normal mouse skin was assessed by combining tritiated thymidine [3H]TdR autoradiography with enzyme histochemistry. After injection of [3H]TdR, ear skin was treated in two ways. Epithelial sheet preparations were stained for the presence of non‐specific esterase and cytospin preparations of epithelial cell suspensions were stained for beta‐glucuronidase activity. The labelling index (p± SE mean) for cytospins, 40 min after injecting [3H]TdR, was 1.6 ± 0.15%, doubling to 3–4% from 7–17 days after injection. The sheet preparations showed the proportion of label attributable to paired Langerhans’cells rising from 18% at 40 min after injection, to approximately 45%, on days 1–4 after injection. These results suggest that the proliferation of Langerhans’cells in normal mouse skin might be higher than was previously thought to be the case.

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Section: Discussionmentioning

confidence: 82%

DNA synthesis in Langerhans’cells of mouse ear epithelium revealed by tritiated thymidine autoradiography and histochemical staining for non‐specific esterase and beta‐glucuronidase activity

Hume

Moore

1989

Cell Proliferation

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“…Discussion LC are mesenchymal, antigen-presenting cells found within mammalian epithelia that originate from stem cells residing in the bone marrow. Some studies have shown thatLC of both animal (3)(4)(5) and human epidermis (6,7) are capable of DNA synthesis. The work of Czemielewski et al (8), using normal human skin xenografted onto nude mice, suggested that human LC are a cycling cell population with a percentage of LC being in the S (1.3-3.3%) and G2/M (1.0-2.5%) phases of the cell cycle and comparable to that of keratinocytes.…”

Section: Resultsmentioning

confidence: 99%

“…The studies of Katz et al (1) and Frelinger et al (2) convicingly showed that LC are derived from, and repopulated within the skin by, a pool of precursors residing in the bone marrow. There has also been accumulating evidence to support the fact that mature epidermal LC are capable of DNA synthesis, as ascertained by 3H-thymidine and BrdU-incorporation (3)(4)(5). Up till now, however, extremely few ultrastructural reports have documented LC mitotic division within human epidermis.…”

Section: Introductionmentioning

confidence: 99%

Electron‐microscopic Observation of a Human Epidermal Langerhans Cell in Mitosis

Kanitakis

Hoyo

Perrin

et al. 1993

The Journal of Dermatology

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Langerhans cells are dendritic cells of the epidermis originating from bone marrow precursors which may exceptionally undergo mitosis within the skin. We report herein an electron-microscopic observation of a dividing LC within a seemingly hyperproliferative human epidermis. This observation further underlines the self-reproducing capacity of LC in situ and suggests that LC may respond to the same mitogenic stimuli as keratinocytes.

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“…On the other hand, studies indicating that LC can divide in the epidermis have been reported. Incorporation of 3H-thymidine into the nuclei of LC was observed in situ, although with a low labeling index (17,22). Langerhans cells in mitosis have been detected by electron microscopy (14).…”

Section: Discussionmentioning

confidence: 99%

Detection of distinct subpopulations of langerhans cells by flow cytometry and sorting

Vaigot¹,

Czernielewski²,

Pruniéras

1985

Cytometry

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Flow cytometry was found to be a very appropriate tool for the study of Langerhans cells (LC), which represent a minor cell population (2-3%) of human epidermis, and allowed us to obtain new phenotypic, functional, and cell cycle data on these rare cells.The phenotypic analysis of cell surface antigens demonstrates the existence of two subpopulations of LC: the former is HLADR+and OKT 6 + (about 90% of total HLA-DR+ cells) and the latter is HLA-DR+ and OKT 6-(about 10% of total HLA-DR+ cells). These subpopulations of LC are both able to stimulate the proliferation of peripheral blood lymphocytes (PBL) in the presence of keratinocytes i.e., in mixed skin lymphocyte reaction (MSLR).Analysis of the cell cycle could be performed on OKT 6+ LC. Results show that they can be found in the various phases of the cell cycle, suggesting that the large majority of Langerhans cells are able to proliferate in situ in normal human epidermis.Key terms: Flow cytometry, Langerhans cells, double fluorescence, cell cycle, sterile sorting Langerhans cells (LC) are mononuclear, dendritic cells located among suprabasal keratinocytes in the epidermis of all mammalian species. Although they represent a minor population of epidermal cells (2-3%), great interest has been focused recently on them because their immunological role has become evident (23,261: 1) They express class 11 antigens and bear receptors for the Fc portion of IgG and several complement components (13,20,25); and 2) even more interestingly, LC are necessary for the induction by epidermal cells (EC) of autologous and allogeneic T-cell activation in the mixed skin cell lymphocyte reaction (MSLR) (3,8,26). Recently, human LC were also shown to be specifically stained with OKT 6 monoclonal antibody (MAb) (10). However, the functional importance of OKT 6 vs. HLA-DR determinants is still unclear. A serious hindrance to LC studies is their low number in epidermis. Their physical separation has been attempted by rosetting and monolayer techniques, with enrichment of 18 to 35% (8,26). Two other attempts have been made (19,211 with cell sorters, allowing purities higher than 80%. In the present study, we used the double immunofluorescence technique and sterile flow cytometry (FCM) sorting to analyze their antigenic determinants and their functional role.On the other hand, few data exist on the origin and renewal of LC in human epidermis. Katz et al. (12) have shown that LC originate in mice from bone marrow precursors, although some data suggest their possible renewal in the skin by mitosis (9, 14, 17). The FCM has proved to be a powerful tool in cell cycle studies (2) including those of murine and human keratinocytes (1,6). However, the low number of LC in the epidermis has limited until now the possibility of studying their kinetics accurately. To investigate whether LC go through the cell cycle, we chose three different approaches, using the cell sorter: 1) DNA staining of enriched LC suspensions, 2) simultaneous staining of LC membrane antigens and DNA, and 3) direct sorting ...

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Zur autoradiographischen Markierung von Langerhans-Zellen mit 3H-Thymidin

Cited by 32 publications

References 8 publications

DNA synthesis in Langerhans’cells of mouse ear epithelium revealed by tritiated thymidine autoradiography and histochemical staining for non‐specific esterase and beta‐glucuronidase activity

DNA synthesis in Langerhans’cells of mouse ear epithelium revealed by tritiated thymidine autoradiography and histochemical staining for non‐specific esterase and beta‐glucuronidase activity

Electron‐microscopic Observation of a Human Epidermal Langerhans Cell in Mitosis

Detection of distinct subpopulations of langerhans cells by flow cytometry and sorting

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