Zur Frage der gleichzeitig vorhandenen proteolytischen und antiproteolytischen Aktivität im Sputum bei obstruktiven Atemwegserkrankungen

Hochstrasser, K; Hochgesand, K.; Rasche, B.

doi:10.1159/000193594

Cited by 15 publications

(7 citation statements)

References 7 publications

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“…In chronic bronchitis, slow mucociliary clearance [4], bronchostenosis and abnor mal mucus composition [5,6] lead the in haled organic particles to have a longer dwelling time for physicochemical inter action.…”

Section: Introductionmentioning

confidence: 99%

Biodegradability of Inhaled Organic Particles in Patients with Chronic Bronchitis

Todisco¹,

Dottorini²,

Fiorucci

et al. 1987

Respiration

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2 h after the inhalation of monodispersed ^99mTc-labeled autologous spherocytes and of commercial human albumin microspheres (HAM), 7 patients with chronic bronchitis underwent bronchofibroscopy. The fate of organic particles along the tracheobronchial tree was verified by scanning electron microscopy and the proteolytic activity (trypsin and PZ peptidase) in mucus samples was assessed. Significant proteolytic activity was detected in bronchial secretions. Thereafter in vitro digestion of labeled spherocytes and HAM was verified after exposure to increasing concentrations of trypsin. While in vitro a similar time-course of tryptic digestion of both particles was observed, in vivo spherocytes seem to be less vulnerable to enzymatic digestion. These findings add another unexpected variable, which may influence the reproducibility of radioaerosol lung mucociliary clearance measurements, and improve its standardization.

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Section: Introductionmentioning

confidence: 99%

Biodegradability of Inhaled Organic Particles in Patients with Chronic Bronchitis

Todisco¹,

Dottorini²,

Fiorucci

et al. 1987

Respiration

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“…Von mehreren Autoren wurden proteolytische Fer mentaktivitäten im Bronchialschleim gefunden [5,6,[10][11][12][13]15]. Lieberman et al [11] sahen diese als ausschliesslich von den zerfallenen Leukozy- Eingegangen: 3.…”

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“…Lieberman et al [11] sahen diese als ausschliesslich von den zerfallenen Leukozy- ten im Entzündungsgebiet herstammend an. Welche Rolle die Vielzahl der Bakterienproteasen spielt, wird diskutiert [4,6], Mit Hilfe der gut messbaren Inhibitorkonzentration [2] wurde versucht, die Proteaseaktivität zu quanti fizieren [5,6,12,15,17].…”

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“…Auch das Verhältnis des freien zu bereits an Proteasen gebundenen Inhibitors wird bestimmt [5,6,17].…”

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“…H ochstrasser et al [5,6] haben gezeigt, dass proteolytische und Proteolyse inhibierende Aktivitäten im Bronchialschleim bei chronischer Bronchitis gleichzeitig Vorkommen. Bei einem Fall von hochgradigem ai-Antitrypsinmangel konnte die Inhibitorkapazität im Bronchialschleim übereinen länge ren Zeitraum gemessen werden.…”

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Schleimhautspezifische Proteaseinhibitoren im Bronchialschleim bei schwerer chronisch obstruktiver Bronchitis und bei α<sub>1</sub>-Antitrypsinmangelsyndrom

Rasche¹,

Hochstrasser²,

Marcic³

et al. 1975

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In the bronchial mucus of 40 patients with chronic obstructive airway diseases we measured proteolytic activities, the total protein concentrations, α₁-antitrypsin, α₁-antichymotrypsin, and the free and bound proteinase inhibitors together with the total proteinase inhibition against trypsin and chymotrypsin. Without exception we always found free proteinase inhibitors together with proteolytic activities. The free-to-bound inhibitor rate was approximately 1:1. α₁-Antitrypsin and αi-antichymotrypsin was measured in sputum only in very low concentrations. One patient with α₁-antitrypsin deficiency had no α₁-antitrypsin, but high concentrations of total proteinase inhibitor – free and bound being in the same relation – in his bronchial mucus. In the alveolar part of the lung, the humoral proteinase inhibitors were effective. In the bronchial part of the lung the specific mucosal inhibitors had the decided importance. The proteinase inhibition of the mucosa-specific inhibitors is probably of great importance for the pathogenesis of airway obstruction, while the humoral proteinase inhibitors are responsible for the pathogenesis of emphysema.

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Aggression und Abwehr am Beispiel der Lunge

Ulmer¹,

Islam²,

Zimmermann³

et al. 1982

Verhandlungen Der Deutschen Gesellschaft Für Innere Medizin

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Zur Frage der gleichzeitig vorhandenen proteolytischen und antiproteolytischen Aktivität im Sputum bei obstruktiven Atemwegserkrankungen

Cited by 15 publications

References 7 publications

Biodegradability of Inhaled Organic Particles in Patients with Chronic Bronchitis

Biodegradability of Inhaled Organic Particles in Patients with Chronic Bronchitis

Schleimhautspezifische Proteaseinhibitoren im Bronchialschleim bei schwerer chronisch obstruktiver Bronchitis und bei α<sub>1</sub>-Antitrypsinmangelsyndrom

Aggression und Abwehr am Beispiel der Lunge

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