Zur Kenntnis der Askosporenbildung bei Torulopsis pulcherrima (Lindner) Sacc

The Yeasts

“…Since spore-formation is often delayed, cultures should be examined repeatedly over a period of several weeks before they are to be labelled as asporogenous. Windisch (1938) reported spore formation in Torulopsis pulcherrima only after the agar cultures had dried considerably, and Todd and Herrmann (1936) had the same experience with Debaryomyces neoformans. Fuchs (1935) found that with old laboratory strains which had quit forming spores this character could be revived by growing them on wort agar, transferring the young growth to plaster blocks, then back to wort agar, and so on.…”

“…It was noted that white variants from the original red yeast are much more stable than the parent stock; that white variants arise from red usually as sectors, i.e., at the edge of the growing colony; while in those rare cases where white reverted to red, this occurred as secondary colonies in the center of the colony. The contention that the variations were independent of any sexual process is not certain in view of the later observation by Windisch (1938) that Torulopsi8 pulcherrima does form ascospores by isogamous conjugation. Spore formation occurred in the presence of a species of Penicillium, and in very old agar cultures dried to such a degree that the growth adhered to the agar.…”

“…From time to time, as with other imperfect fungi, spores are found in yeasts formerly believed to be asporogenous. Recent observations of this sort are the discovery of spores in a strain of Torulopsis pulcherrima by Windisch (1938), and the observation by Niehaus (1932) and by Dvornik (1938) that all strains of Kloeckera form spores when freshly isolated. Diddens and Lodder (1939b) record the discovery of spores in the following yeasts formerly reported as asporogenous, with the changes in nomenclature in-dicated: Monilia pinoyisimilis and Monilia pseudotropicalis = Saccharomyces fragilis; Monilia macedoniensis = Saccharomyces macedoniensis; Monilia javanica = Hansenula anomala; Candida pelliculosa = Hansenula javanica.…”