Zur Massenspektroskopie der Aflatoxine

Aflatoxins are detected and determined by analytical procedures based on physical and chemical properties, e.g., ultraviolet absorbance, fluorescence, solubility and chromatographic retention times during thin layer (TLC) or liquid chromatography. For acceptance of analytical results based on these properties, especially for regulatory purposes, proof of identity of the compound being measured is essential. Numerous tests have been devised for confirmation of identity. Included are tests based on toxicological effects observed in the duckling, zebrafish, chick embryo, Bacillus megaterium and many other species; chemical tests based on formation of derivatives such as the acetates and water adducts; and tests based on color changes of TLC spots after contact with spray reagents, e.g., sulfuric acid. All of the foregoing have inherent uncertainties in interpretation of identity. On the other hand, mass spectrometry (MS) is one of the most specific methods of identification available;however, it has been difficult to apply at the low concentrations at which aflatoxins are routinely detected. In this paper, the confirmation techniques for aflatoxins are placed in historical perspective and are reviewed and evaluated. A recently developed procedure for the application of negative ionization MS for the confirmation of identity of aflatoxins in foods or feeds at concentrations as low as I0 ng/g is described. This procedure consists of isolation of the aflatoxin by Association of Official Analytical Chemists' methods, purification by preparatory 2-dimensional TLC, in situ elution of the aflatoxin TLC spot and analysis of the sample by negative ion chemical ionization MS using a direct insertion probe. ABSTRACTThe solvent-saving procedure devised by Ports using a small chromatographic tube (Bio-Rad Laboratories glass Econo-Column, 10 mm id • 300 mm long) has been adapted and extended for use in modifications of the Official AOAC procedure for quantitative determination of aflatoxins in corn, peanuts, soybeans, coconut and pistachios. Thirty mL of each of 3 solvents for column washes was used instead of the 150 mL specified by the Official CB Method. The analytical aliquot was also reduced 80%, but sample size and extracting solvent volume were not changed, so that there was no loss in sensitivity. Toxins ranging from 3 to over 1,000 jag/g of sample were quantitated after dean-up using both procedures with no statistically significant difference between results.

show abstract

Zur Massenspektroskopie der Aflatoxine

Cited by 4 publications

References 11 publications

Mycotoxine, insbesondere Aflatoxine

Mycotoxine, insbesondere Aflatoxine

Einsatzmöglichkeiten der Massenspektrometrie bei der Überwachung von Lebensmitteln und Bedarfsgegenständen. II

Confirmation of identity of aflatoxins

Contact Info

Product

Resources

About