Zur Methodik der Serum- und Gewebslipase

Rona, Peter A.; Lasnitzki, A.

doi:10.1007/bf01735578

Cited by 5 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sie basieren auf der Bestimmung von CO,, das durch Buttersaure, die wiihrend der Lipolyse aus Tributyrin entsteht, aus einer NaHC0,-Pufferlosung freigesetzt wird [61,62]. Aufgrund der geringen Empfindlichkeit und der ungenugenden Reproduzierbarkeit finden sie heute kaum noch Anwendung.…”

Section: Manometrische Methodenunclassified

Übersichtsartikel. Die Charakterisierung mikrobieller Lipasen. 1. Mitt. Zur Bestimmung der Lipaseaktivität

Bariszlovich

Meusel

Tülsner

1990

Nahrung

View full text Add to dashboard Cite

In the selection of an appropriate method for activity determination of lipases existing technical equipment, kind of enzymes, number of samples investigated (e.g. in routine analysis), and expected sensitivity range have to be taken into account. Titrimetric methods and above all copper salt methods with their high detection sensitivity are the most suitable procedures for activity determination of lipases used in laboratories and institutions without equipment for radiochemical analysis.

show abstract

Section: Manometrische Methodenunclassified

Übersichtsartikel. Die Charakterisierung mikrobieller Lipasen. 1. Mitt. Zur Bestimmung der Lipaseaktivität

Bariszlovich

Meusel

Tülsner

1990

Nahrung

View full text Add to dashboard Cite

show abstract

“…Choline Esterase Activity of Horse Serum. The choline esterase activity of the horse serum was determined manometrically [Rona and Lasnitzki, 1924;Ammon, 1934] using vessels with one side bulb in which the enzyme was placed. The medium was bicarbonate Ringer saturated with a gas mixture containing 5 per cent.…”

Section: Serummentioning

confidence: 99%

Untitled

1937

Exp Physiol

View full text Add to dashboard Cite

OCH2CH2N(CH3) + PHOSPHORYLCHOLINE, 0= P-0 is a choline ester-OH which forms a part of the phosphatide molecule, and as such occurs in nearly all organs. It probably often exists in the free state, being liberated from the phosphatides by enzymic hydrolysis. An investigation of some of the chemical, biochemical, and physiological properties of phosphorylcholine was carried out in view of the physiological activity of many choline esters, especially acetylcholine, and their significance as chemical transmitters of nerve impulses. Synthesis: 2 Phosphorylcholine was synthesised from choline chloride and phosphorus pentoxide, the solvent being 100 per cent. phosphoric acid [phosphorylation method of Manaka, 1931]. 2*4 g. choline chloride, dried over phosphorus pentoxide, were dissolved in

show abstract

“…Esterase was determined by the method of Rona & Lasnitzki (1924) in which the COs, displaced from a bicarbonate buffer by the hydrogen ions liberated during the hydrolysis of tributyrin, is measured in a standard Warburg manometer. Details of the test have been published by Rossiter & Wong (1949).…”

mentioning

confidence: 99%

Surface‐active substances and the liberation of enzymes from rabbit polymorphonuclear leucocytes

Rossiter¹

1949

The Journal of Physiology

View full text Add to dashboard Cite

Much can be learned of the function of the white cell by studying its constituent enzymes. Our present knowledge of white-cell enzymes has been reviewed in papers by Barnes (1940) and Rebuck (1947 Rossiter, 1948, esterases (Rossiter & Wong, 1949) and, more recently, the /3-glucuronidases of rabbit polymorphonuclear leucocytes. A preliminary report of these experiments has already appeared . METHODSCell preparation8. Polymorphonuclear leucocytes were obtained from the peritoneal cavity of the rabbit by the method of de Haan (1918). A warm sterile solution of 0.9% NaCl (300-500 ml.) was run into the peritoneal cavity and drained off 3-4 hr. later into a beaker containing a few grains of heparin. Alternatively, the solution was left in the animal overnight and a further quantity was run in the following morning and withdrawn immediately. In each instance the resulting opalescent fluid contained 2000-16,000 leucocytes/cu.mm., at least 95 % of which were polymorphonuclear.Alkaline pho8phatase e8timation. Alkaline phosphatase was determined by the method of King & Armstrong (1934) in which the phenol, liberated by the hydrolysis of disodium phenyl phosphate, is estimated by the method of Folin & Ciocalteu (1927). Sufficient magnesium chloride was added to each tube to make the final magnesium concentration 0 001 M. Details of the test have been published by Cram & Rossiter (1949). The results were recorded as the amount of phenol (in mg.) liberated, under the standard conditions of the test, by 100 ml. enzyme in 1 hr.

show abstract

Zur Methodik der Serum- und Gewebslipase

Cited by 5 publications

References 0 publications

Übersichtsartikel. Die Charakterisierung mikrobieller Lipasen. 1. Mitt. Zur Bestimmung der Lipaseaktivität

Übersichtsartikel. Die Charakterisierung mikrobieller Lipasen. 1. Mitt. Zur Bestimmung der Lipaseaktivität

Untitled

Surface‐active substances and the liberation of enzymes from rabbit polymorphonuclear leucocytes

Contact Info

Product

Resources

About