Zur Pathologie des Diabetes mellitus der wei�en Maus bei der EMC-Virusinfektion

Münterfering, H.

doi:10.1007/bf00543156

Cited by 18 publications

(7 citation statements)

References 57 publications

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“…Male NMRI mice (30--38 g) were inoculated by subcutaneous injections of 0.2 ml of the virus-pool. Glucose tolerance tests performed in the infected mice, remained unchanged during the first three days, corresponding to earlier findings [7].…”

Section: Methodssupporting

confidence: 85%

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Synthesis of proinsulin and large glucagon immunoreactivity in isolated langerhans islets from EMC-virus infected mice

1975

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The protein synthesis in normal and in EMC-virus infected mouse islets of Langerhans was investigated. Mouse large glucagon immunoreactivity was determined by an immunoassay after chromatographic separation. It was characterized as a peptide of 16 000 MW with in intracellular half-life of 35-45 min. The proportional reduction of void volume proteins, large glucagon immunoreactivity and proinsulin synthesis after infection shows, that both alpha- and beta-cells are damaged by the virus. A reduction in the synthesis of the three protein fractions was already found 6 hrs after inoculation of the virus and remained nearly constant for 48 hrs. An almost complete breakdown of protein synthesis occurred 60 to 70 hrs after infection and was paralleled by the first light microscopic changes in the islets. The stimulation of proinsulin synthesis by glucose was preserved for 48 hrs after EMC-virus infection.

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Section: Methodssupporting

confidence: 85%

“…Three days after the inoculation of EMC-virus in mice beta-cell necrosis was observed, five days after infection round cell infiltration of the islets of Langerhans appeared [7]. This study presents experimental data on the synthesis of proinsulin (PI) and large glucagon immunoreactivity (LGI) in isolated islets of Langerhans from EMC-virus infected mice.…”

mentioning

confidence: 95%

Synthesis of proinsulin and large glucagon immunoreactivity in isolated langerhans islets from EMC-virus infected mice

1975

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“…Our results support the concept that one main feature of EMC-virus induced diabetes is B-cell necrosis [5,8,20,21]: the total volume of insulinfluorescent cells was greatly decreased after a few days post-infection, it remained low in hyperglycaemic animals but returned to normal in animals that recovered from hyperglycaemia. Insulin content of the pancreas has been shown to follow a similar pattern, as well as B-cell responsiveness to secretory stimuli [22].…”

Section: ) the Architecture Of The Islets Of Langerhans Was Profoundsupporting

confidence: 87%

“…Viral infection in experimental animals has proved a valuable model system of diabetes. In some strains of mice, infection with the M variant of the encephalomyocarditis (EMC) virus induces a diabetes-like syndrome characterized by initial hypoglycaemia, soon followed by hyperglycaemia, hypoinsulinaemia, glycosuria, polydipsia and polyphagia [1][2][3][4][5][6][7]. In DBA/2 male mice, 60 to 80% of the infected animals develop hyperglycaemia.…”

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confidence: 99%

Virus-induced diabetes in mice: A quantitative evaluation of islet cell population by immunofluorescence technique

et al. 1978

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The endocrine cell populations of pancreatic islets in encephalomyocarditis (EMC)-virus infected mice were assessed quanititatively by immunofluorescence using specific antisera against 4 islet hormones. A marked reduction of the volume of insulin-containing (B-) cells (up to one tenth of control values) was observed at all stages studied in the hyperglycaemic mice. This was accompanied by the inversion of the normal ratio between B- and non B-cells. The volume of the latter cell types was also modified at different time points after infection: glucagon-cells were augmented 14 days after infection; PP-cells were decreased 2--3 days and 21 days after infection; somatostatin-cells decreased to one-fourth of control values in hyperglycaemic animals 21 days after infection. The latter results suggest that non B-cells are also involved in islet reaction to virus infection.

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“…The M variant of EMC virus induces a diabetes mellitus-like disease in several strains of mice [1][2][3][4][5][6][7]. In most of these strains, hyperglycaemia is observed in infected male animals, but occurs inconsistently, or not at all, in females [1,8].…”

mentioning

confidence: 99%

Testosterone effect on experimental diabetes mellitus in encephalomyocarditis (EMC) virus infected mice

1980

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A diabetes mellitus-like disease occurs in male DBA/2 mice infected with the M variant of the encephalomyocarditis virus. Female mice of this strain sustain systemic infection, but rarely exhibit hyperglycaemia. The diabetogenic effects of the virus were studied in 3 groups of adult DBA/2 males-castrates, castrates treated with testosterone, and sham-operated controls. After infection, pancreatic insulin concentrations decreased precipitously to approximately 10% of control values in intact males and castrates treated with testosterone; hyperglycaemia occurred concomitantly in both groups. In contrast, untreated castrates failed to develop hyperglycaemia and the effect on the insulin content of the pancreas was less striking.

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Zur Pathologie des Diabetes mellitus der wei�en Maus bei der EMC-Virusinfektion

Cited by 18 publications

References 57 publications

Synthesis of proinsulin and large glucagon immunoreactivity in isolated langerhans islets from EMC-virus infected mice

Synthesis of proinsulin and large glucagon immunoreactivity in isolated langerhans islets from EMC-virus infected mice

Virus-induced diabetes in mice: A quantitative evaluation of islet cell population by immunofluorescence technique

Testosterone effect on experimental diabetes mellitus in encephalomyocarditis (EMC) virus infected mice

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