Zur Problematik der elektronenmikroskopischen Darstellung der Succinodehydrogenase mit dem osmiophilen Tetrazoliumsalz TC-NBT

Guinea pig hearts isolated by the Langendorff technique were perfused with a nitro-blue tetrazolium salt solution. In spite of a homogeneous dark-blue staining at the macroscopic examination, light-microscopic studies showed a spotty staining of the myocardial fibers. Since no microscopic alterations were found which could explain the unstained areas, ultramicroscopic studies were undertaken. At ultrastructural level, striking alterations of myocardial cells were observed. They consisted of mitochondrial lesions, alterations of the transversal tubuli, and intercalated discs. Arteriolar narrowing and capillary edema were found, suggesting that pathological changes in the microcirculation could be a possible reason for the spotty staining of the myocardial fibers. Some speculations on the nature and localization of the formazan granules are also reported.

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Electron microscopical enzyme histochemistry on unfixed tissues and cells. Bridging the gap between LM and EM enzyme histochemistry

Schellens

Vreeling-Sindelárová

Frederiks

2003

Acta Histochemica

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Zur Problematik der elektronenmikroskopischen Darstellung der Succinodehydrogenase mit dem osmiophilen Tetrazoliumsalz TC-NBT

Cited by 3 publications

References 29 publications

Zur Methodik der elektronenmikroskopischen Darstellung der Succinat-Tetrazoliumsalzreduktase mit TC-NBT

Zur Methodik der elektronenmikroskopischen Darstellung der Succinat-Tetrazoliumsalzreduktase mit TC-NBT

Light and electron-microscopic studies on guinea pig hearts after perfusion with nitro-blue tetrazolium (NBT)

Electron microscopical enzyme histochemistry on unfixed tissues and cells. Bridging the gap between LM and EM enzyme histochemistry

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