Zur Rolle von Monocyten bei der antigenbedingten Lympho-cytentransformation in vitro

Macromolecular Inhibitor of Lymphocyte Transformation in Serum From Patients With Chronic Renal Failure

“…In the present study the depressive activity was relatively greater after dialysis of the serum; the faetor(s) must therefore' have a molecular weight of at least 10,000. The routine use of autologous serum or plasma in eultures of patients' lymphocytes, as in the experiments of Huber et al (1969) does not allow a distinction to be drawn between cellular defects and the effect of depressive humoral factors. Kasakura and Lowenstein (1967) noted depression by uraemic plasma of the transformation of normal lymphocytes in response to allogeneic lymphocytes but not in response to PHA.…”

“…such as diminished or absent delayed skin reactivity (Wilson, Kirkpatrick and Talmage. 1965;Huber, Pastner, Dittrich and Braunsteiner, 1969) and slowing of allograft rejection (Dammin, Couch and Murray, 1957). Although lymphopenia may contribute to this depression, the function of the cells themselves may be impaired, either because of a cellular defect or because the "uraemic" environment is unfavorable.…”

“…Kasakura and Lowenstein (1967) and Daniels, Remmers, Sarles, Fish, Cobb, Levin and Ritzmann (1970) reported that the responses of patients' lymphocytes to phytohaemagglutinin (PHA) were normal. When patients' lymphocytes were cultured in autologous serum or plasma, depressed responses were found to PHA (Huber et al, 1969), to allogeneic leucocytes (Elves. Israels and Colling, 1966;Kasakura and Lowenstein, 1967) and to specific antigens (Huber et al, 1969).…”

“…When patients' lymphocytes were cultured in autologous serum or plasma, depressed responses were found to PHA (Huber et al, 1969), to allogeneic leucocytes (Elves. Israels and Colling, 1966;Kasakura and Lowenstein, 1967) and to specific antigens (Huber et al, 1969). A depression of the response of normal lymphocytes to PHA by plasma or serum from uraemic patients was observed by Silk (1967) and by Newberry and Sanford (1971) but was not detected by Kasakura and Lowenstein (1967).…”