Zur Wirkung von Dinitrokresol und Thiouracil auf den Zellstoffwechsel

Locker, A.; Siedek, H; Spitzy, K. H.

doi:10.1007/bf00244548

Cited by 11 publications

(3 citation statements)

References 42 publications

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“…Rotenone and DNOC have a mode of action in a primary metabolic pathway, the oxidative phosphorylation [38, 39]. Both substances caused an effect on a higher number of proteins in the proteome compared to Diclofenac with the primary mode of action in a secondary biochemical pathway (inhibition of cyclooxygenases) [36].…”

Section: Discussionmentioning

confidence: 99%

“…Hereby, the main questions to be answered were whether toxicity related responses can be detected at low-dose ranges in the proteome profiles of treated eleuthero-embryos, whether exposure with different model substances can be discriminated at the proteome level and whether possible candidate protein biomarkers for predictive effect diagnosis might be proposed. Therefore, proteomics was established for zebrafish eleuthero-embryos and proteomic analyses were performed with three different compounds, including two insecticides, affecting primary metabolic pathways (oxidative phosphorylation), Rotenone and DNOC [ 38 , 39 ], and the pharmaceutical Diclofenac, with an anti-inflammatory mode of action [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…Analysing the number of changed proteins the effect analysis at the proteome level could give information on how basal the affected metabolic pathways are in contrast to classical toxic endpoints. Rotenone and DNOC have a mode of action in a primary metabolic pathway, the oxidative phosphorylation [ 38 , 39 ]. Both substances caused an effect on a higher number of proteins in the proteome compared to Diclofenac with the primary mode of action in a secondary biochemical pathway (inhibition of cyclooxygenases) [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

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Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals

Hanisch

Küster

Altenburger

et al. 2010

International Journal of Proteomics

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Studies using embryos of the zebrafish Danio rerio (DarT) instead of adult fish for characterising the (eco-) toxic potential of chemicals have been proposed as animal replacing methods. Effect analysis at the molecular level might enhance sensitivity, specificity, and predictive value of the embryonal studies. The present paper aimed to test the potential of toxicoproteomics with zebrafish eleutheroembryos for sensitive and specific toxicity assessment. 2-DE-based toxicoproteomics was performed applying low-dose (EC10) exposure for 48 h with three-model substances Rotenone, 4,6-dinitro-o-cresol (DNOC) and Diclofenac. By multivariate “pattern-only” PCA and univariate statistical analyses, alterations in the embryonal proteome were detectable in nonetheless visibly intact organisms and treatment with the three substances was distinguishable at the molecular level. Toxicoproteomics enabled the enhancement of sensitivity and specificity of the embryonal toxicity assay and bear the potency to identify protein markers serving as general stress markers and early diagnosis of toxic stress.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals

Hanisch

Küster

Altenburger

et al. 2010

International Journal of Proteomics

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�ber Stoffwechselunterschiede zwischen den Geweben kalt- und warmbl�tiger Tiere

Locker

1962

Pfl�gers Archiv

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Reactions of metabolizing systems following experimental influences, stimulation or injury

Locker

1964

Helgolander Wiss. Meeresunters

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Reactions of metabolizing systems following experimental influences, stimulation or injury (Basic problems of quantitative biology of metabolism). Metabolizing systems which follow the kinetics of open systems reveal characteristic reactions due to experimental influences, stimulation or injury. In the time-course of such reactions single or multiple transition states are observed; with a constant time parameter, a behaviour becomes manifest which was described long ago by various laws (Wrsr~-FEcmqE~-Iaw, ARNDT-ScHuLZlaw, law of initial value after WiLI)rl~). These laws are derivable from the theory of open systems. Enzyme kinetics provide us with simple models obeying these laws; they are, however, also verified by reactions of metabo.lizing systems of either level or organization. Specific examples of these reactions are presented from the areas of experimental liver-injury, resting and activity state, as well as metabolic differences between cold-and warmblooded animals. The limitations of quantitative methods in biology are emphazised. EINLEITUNG Aufgabe und Ziel unserer Betraehtung Mit dem Ausblick auf das Ziel einer durchgehend mathematischen Darstellung sucht die quantitative Biologie Beziehungen zwischen Wirkfaktoren verschiedenster Art und biologischen Prozessen beziehungsweise Systemen zu beschreiben. Die ,,Quantitative Biologie des Stoffwechsels", mit der wit uns bier im besonderen auseinandersetzen wollen, l~if~t sich daher in bestimmter Weise yon der Biochemie unterscheiden; bei ihr stehen nicht die stoffliche Natur der Reaktionsteilnehmer oder die chemischen Ums~tze, d. h. die Wandlungen der chemischen Strukturen, im Vordergrund der Betrachtung. Die Ergebnisse der Biochemie k~Snnen gewissermaf~en als Rahmen aufgeraint werden, innerhalb dessert untersucht werden soll 1. wie sich die Reaktionen (vor allem quantitativ, d. h. hinsichtlich ihrer St~irke und Geschwindigkeit) unter bestimmten Beeinfiussungen ~indern und 2. was unter diesen Einfltissen yon allgemeinen M6glichkeiten realisiert wird.

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Zur Wirkung von Dinitrokresol und Thiouracil auf den Zellstoffwechsel

Cited by 11 publications

References 42 publications

Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals

Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals

�ber Stoffwechselunterschiede zwischen den Geweben kalt- und warmbl�tiger Tiere

Reactions of metabolizing systems following experimental influences, stimulation or injury

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