Zwint-1 is a novel Aurora B substrate required for the assembly of a dynein-binding platform on kinetochores

Kasuboski, James M.; Bader, Jason R.; Vaughan, Patricia S.; Tauhata, Sinji Borges Ferreira; Winding, Michael; Morrissey, Meghan A.; Joyce, Michelle V.; Boggess, Bill; Vos, Larissa J.; Chan, Gordon K.; Hinchcliffe, Edward H.; Vaughan, Kevin T.

doi:10.1091/mbc.e11-03-0213

Cited by 52 publications

(57 citation statements)

References 31 publications

(69 reference statements)

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“…In conjunction with INCENP, Borealin and Survivin, Aurora B/C kinases form the chromosome passenger complex and regulate various chromosome dynamics, including chromosome congression, alignment, and segregation. 33 Given that Zwint-1 is required for proper function of Aurora B/C kinases during chromosome segregation in both mitosis and meiosis, 34,35 it could be possible that chromosome defects following BECN1 knockdown might be due to the improper function of Aurora B/C kinases. The notion that BECN1 interacts with Survivin further suggests a possible association between BECN1 and Aurora B/C kinases.…”

Section: Discussionmentioning

confidence: 99%

Beclin-1 knockdown shows abscission failure but not autophagy defect during oocyte meiotic maturation

et al. 2016

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Cytokinesis is the final step in cell division that results in the separation of a parent cell into daughter cells. Unlike somatic cells that undergo symmetric division, meiotic division is highly asymmetric, allowing the preservation of maternal resources for embryo development. Beclin-1/BECN1, the mammalian homolog of yeast Atg6, is a key molecule of autophagy. As part of a class III phosphatidylinositol 3-kinase (PI3K-III) complex, BECN1 initiates autophagosome formation by coordinating membrane trafficking. However, emerging evidence suggests that BECN1 regulates chromosome segregation and cytokinesis during mitosis. Thus, we investigated the function of BECN1 during oocyte meiotic maturation. BECN1 was widely distributed during meiotic maturation forming small vesicles. Interestingly, BECN1 is also detected at the midbody ring during cytokinesis. Depletion of BECN1 impaired the cytokinetic abscission, perturbing the recruitment of ZFYVE26 at the midbody. Similar phenotypes were observed when PI3K-III activity was inhibited. However, inhibition of autophagy by depleting Atg14L did not disturb meiotic maturation. Therefore, our results not only demonstrate that BECN1 as a PI3K-III component is essential for cytokinesis, but also suggest that BECN1 is not associated with autophagy pathway in mouse oocytes.

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Section: Discussionmentioning

confidence: 99%

Beclin-1 knockdown shows abscission failure but not autophagy defect during oocyte meiotic maturation

et al. 2016

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“…First, PP1 may antagonize the Mps1-dependent phosphorylation of KNL-1 by dissociating the checkpoint components Bub1 and Bub3 191–193 . Second, PP1 reverses the Aurora B-dependent phosphorylation of ZWINT-1 in humans and this promotes the dynein-mediated stripping of SAC components from the kinetochore 194 . Lastly, PP1 dephosphorylation of CENP-E also helps stabilize kinetochore microtubule attachments 102 .…”

Section: Roles Of the Cpc In Early Mitosismentioning

confidence: 99%

The chromosomal passenger complex (CPC): from easy rider to the godfather of mitosis

Carmena

Wheelock

Funabiki

et al. 2012

Nat Rev Mol Cell Biol

794

896

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Preface Successful cell division requires the precise and timely coordination of chromosomal, cytoskeletal and membrane trafficking events. These processes are regulated by the competing actions of protein kinases and phosphatases. Aurora B is one of the most intensively studied kinases. In conjunction with the proteins INCENP, Borealin (also known as Dasra) and Survivin, it forms the Chromosomal Passenger Complex (CPC). This complex targets to different locations at differing times during mitosis,, where it regulates key mitotic events: correction of chromosome-microtubule attachment errors, activation of the spindle assembly checkpoint, and construction and regulation of the contractile apparatus that drives cytokinesis. Our growing understanding of the CPC has seen it develop from a mere passenger riding on chromosomes to one of the main controllers of mitosis.

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“…One candidate for an alternative Mad1 co-receptor is the Rod/Zwilch/Zw10 complex (RZZ), which is present mainly in metazoans 57 . Knl1 and its constitutive binding partner Zwint are required to localize RZZ 81,82 , and RZZ localization may be regulated through Aurora B phosphorylation of Zwint 83 . RZZ is required for stable Mad1 localization in human cells 81 , and the RZZ-associated protein Spindly interacts with Mad1 by coimmunoprecipitation in C. elegans 84 .…”

Section: Part I - Kinetochore Activation Of the Checkpointmentioning

confidence: 99%

Signalling dynamics in the spindle checkpoint response

London

Biggins

2014

Nat Rev Mol Cell Biol

299

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Zwint-1 is a novel Aurora B substrate required for the assembly of a dynein-binding platform on kinetochores

Abstract: This study identifies zwint-1 as a novel substrate for AurB during mitosis. Phosphorylation is required for outer kinetochore assembly during prometaphase. However, zwint-1 dephosphorylation is required at metaphase for checkpoint silencing.

Cited by 52 publications

References 31 publications

Beclin-1 knockdown shows abscission failure but not autophagy defect during oocyte meiotic maturation

Beclin-1 knockdown shows abscission failure but not autophagy defect during oocyte meiotic maturation

The chromosomal passenger complex (CPC): from easy rider to the godfather of mitosis

Signalling dynamics in the spindle checkpoint response

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